林业科学
林業科學
임업과학
SCIENTIA SILVAE SINICAE
2011年
1期
85-94
,共10页
杨树%表达序列标签%抗病相关候选基因%表达谱
楊樹%錶達序列標籤%抗病相關候選基因%錶達譜
양수%표체서렬표첨%항병상관후선기인%표체보
poplar%expressed sequence tags%resistance-associated candidate gene%expression profile
利用黑斑病的高抗无性系美洲黑杨I-69及黑斑病的高感无性系欧美杨I-45为材料建立的2个cDNA文库,随机挑取cDNA克隆进行5'端EST序列测序,共获得有效序列20 023条.序列经聚类分析和拼接后,共获得10 816个Unigene,其中包括3 734个Contig,7 082个独立的Singleton.被注释的8 853个具有同源性匹配序列基因中,按照GO的分子功能、生物过程和细胞组分3个不同分类角度进行分类.在具有功能注释的8 853个Unigene中,选出330个与完成全基因组测序的毛果杨序列进行BLAST分析,结果发现有177个抗病相关候选基因出现在282个Unigene中,其中135个分布于杨树的18个不同连锁群上,其他42个基因位于还没定位的scaffolds上.所测定的这些EST序列为后期在基因组水平上研究杨树黑斑病的水平抗性遗传机制及进一步的相关基因发现奠定基础.
利用黑斑病的高抗無性繫美洲黑楊I-69及黑斑病的高感無性繫歐美楊I-45為材料建立的2箇cDNA文庫,隨機挑取cDNA剋隆進行5'耑EST序列測序,共穫得有效序列20 023條.序列經聚類分析和拼接後,共穫得10 816箇Unigene,其中包括3 734箇Contig,7 082箇獨立的Singleton.被註釋的8 853箇具有同源性匹配序列基因中,按照GO的分子功能、生物過程和細胞組分3箇不同分類角度進行分類.在具有功能註釋的8 853箇Unigene中,選齣330箇與完成全基因組測序的毛果楊序列進行BLAST分析,結果髮現有177箇抗病相關候選基因齣現在282箇Unigene中,其中135箇分佈于楊樹的18箇不同連鎖群上,其他42箇基因位于還沒定位的scaffolds上.所測定的這些EST序列為後期在基因組水平上研究楊樹黑斑病的水平抗性遺傳機製及進一步的相關基因髮現奠定基礎.
이용흑반병적고항무성계미주흑양I-69급흑반병적고감무성계구미양I-45위재료건립적2개cDNA문고,수궤도취cDNA극륭진행5'단EST서렬측서,공획득유효서렬20 023조.서렬경취류분석화병접후,공획득10 816개Unigene,기중포괄3 734개Contig,7 082개독립적Singleton.피주석적8 853개구유동원성필배서렬기인중,안조GO적분자공능、생물과정화세포조분3개불동분류각도진행분류.재구유공능주석적8 853개Unigene중,선출330개여완성전기인조측서적모과양서렬진행BLAST분석,결과발현유177개항병상관후선기인출현재282개Unigene중,기중135개분포우양수적18개불동련쇄군상,기타42개기인위우환몰정위적scaffolds상.소측정적저사EST서렬위후기재기인조수평상연구양수흑반병적수평항성유전궤제급진일보적상관기인발현전정기출.
In an attempt to elucidate the molecular mechanism for resistance of black spot disease in poplar,gene expression profiles in leaves of Populus deltoides'Lus'(I-69/55)and P.euramericana'I-45/51',which were inoculated with the pathogen Marssonina brunnea f.sp.brunnea,were analyzed based on expressed sequence tags (ESTs).A total of 20 023 valid ESTs from the 5'terminal ends derived from corresponding cDNA libraries of the two poplar species were sequenced.Cluster analysis of the 20 023 sequences yielded 10 816 tentative unigenes,including 3 734 contigs and 7 082 singletons.All tentative unigenes were classified by Gene Ontology functional categories.To find resistance-associated candidate genes and locate them on poplar genome,330 unigenes was chosen from 8 853 annotated tentative unigenes,and their BLAST alignment was conducted with Populus trichocarpa assembly,1 77 related candidate resistant genes were found,and they presented in 282 unigenes.Among these genes,there were 1 35 genes located on 18 different linkage groups of poplar genome,and 42 genes located on the different scaffolds.This study supplied a resource of candidate genes for further exploring the genetic mechanism for the host horizontal resistance to the pathogen Marssonina brunnea at the whole genome range,and provided important information for further gene discovery.
