中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2008年
10期
697-700
,共4页
朱耀丰%史本康%徐祗顺%周尊林%张克勤%李永智%王海峰%于胜强%刘玉强
硃耀豐%史本康%徐祗順%週尊林%張剋勤%李永智%王海峰%于勝彊%劉玉彊
주요봉%사본강%서지순%주존림%장극근%리영지%왕해봉%우성강%류옥강
膀胱颈梗阻%转化生长因子β%成纤维细胞生长因子,碱性%逼尿肌%尿液
膀胱頸梗阻%轉化生長因子β%成纖維細胞生長因子,堿性%逼尿肌%尿液
방광경경조%전화생장인자β%성섬유세포생장인자,감성%핍뇨기%뇨액
Bladder neck obstruction%Transforming growth factor beta%Fibrobiast growth factor,basic%Detrusor%Urine
目的 探讨转化生长因子β1(TGFβ1)、碱性成纤维细胞生长因子(bFGF)与大鼠膀胱出口梗阻(BOO)后逼尿肌收缩功能障碍的关系.方法 建立Wistar大鼠BOO模型(BOO 2周组11只;BOO 6周组10只),假手术组(8只)作为对照,测定离体逼尿肌肌条M受体激动剂刺激下收缩力,RT-PCR方法测定逼尿肌中TGFB1 mRNA、bFGF mRNA的表达,ELISA测定尿液中TGFβ1、bFGF 的水平.结果 在1×10-4、1×10-3 mmo/L氯化氨基甲酰胆碱(Carbachol)浓度下BOO 2周组的最大逼尿肌收缩力[(0.96±0.11)、(1.98±0.21)g]大于对照组[(0.85±0.18)、(1.82 ±0.19)g,均P＜0.05];大鼠在1×10-5、1×10-4、1×10-3和1 × 10-2 mmol/L Carbachol浓度下BOO 6周组的最大逼尿肌收缩力[(0.19 ±0.02)、(0.65 ±0.06)、(1.12 ±0.08)和(1.40 ±0.19)g]均小于BOO 2周组[(0.24±0.03)、(0.96 ±0.11)、(1.98 ±0.21)和(2.16 ±0.21)g,均P＜0.05]和对照组[(0.23 ±0.04)、(0.85 ±0.18)、(1.82 ±0.19)和(2.12 ±0.26)g,均P＜0.05].TGFβ1 mRNA在对照组、BOO 2周组、BOO 6周组逼尿肌的表达分别为0.32 ±0.01、0.34 ±0.10和0.72 ±0.21,后两组之间差异有统计学意义(P＜0.01);bFGF mRNA表达分别为0.10±0.05、0.21±0.07和0.38 ± 0.13,组间两两比较,差异均有统计学意义(均P＜0.05).离体逼尿肌收缩力与其TGFβ1 mRNA、bFGF mRNA表达水平呈负相关(均P＜0.05).BOO 6周组尿中TGFβ1表达[(606±216)μg/mol肌酐]明显高于BOO 2周组[(131 ±49)μg/mol肌酐]和对照组[(107 ±22)μg/mol肌酐,均P＜0.05].结论 逼尿肌bFGF mRNA表达随BOO进展持续升高,TGFβ1 mRNA在失代偿阶段表达才明显升高;尿液TGFβ1在大鼠BOO 6周时呈强表达,有可能成为预测BOO后逼尿肌收缩功能的指标.
目的 探討轉化生長因子β1(TGFβ1)、堿性成纖維細胞生長因子(bFGF)與大鼠膀胱齣口梗阻(BOO)後逼尿肌收縮功能障礙的關繫.方法 建立Wistar大鼠BOO模型(BOO 2週組11隻;BOO 6週組10隻),假手術組(8隻)作為對照,測定離體逼尿肌肌條M受體激動劑刺激下收縮力,RT-PCR方法測定逼尿肌中TGFB1 mRNA、bFGF mRNA的錶達,ELISA測定尿液中TGFβ1、bFGF 的水平.結果 在1×10-4、1×10-3 mmo/L氯化氨基甲酰膽堿(Carbachol)濃度下BOO 2週組的最大逼尿肌收縮力[(0.96±0.11)、(1.98±0.21)g]大于對照組[(0.85±0.18)、(1.82 ±0.19)g,均P＜0.05];大鼠在1×10-5、1×10-4、1×10-3和1 × 10-2 mmol/L Carbachol濃度下BOO 6週組的最大逼尿肌收縮力[(0.19 ±0.02)、(0.65 ±0.06)、(1.12 ±0.08)和(1.40 ±0.19)g]均小于BOO 2週組[(0.24±0.03)、(0.96 ±0.11)、(1.98 ±0.21)和(2.16 ±0.21)g,均P＜0.05]和對照組[(0.23 ±0.04)、(0.85 ±0.18)、(1.82 ±0.19)和(2.12 ±0.26)g,均P＜0.05].TGFβ1 mRNA在對照組、BOO 2週組、BOO 6週組逼尿肌的錶達分彆為0.32 ±0.01、0.34 ±0.10和0.72 ±0.21,後兩組之間差異有統計學意義(P＜0.01);bFGF mRNA錶達分彆為0.10±0.05、0.21±0.07和0.38 ± 0.13,組間兩兩比較,差異均有統計學意義(均P＜0.05).離體逼尿肌收縮力與其TGFβ1 mRNA、bFGF mRNA錶達水平呈負相關(均P＜0.05).BOO 6週組尿中TGFβ1錶達[(606±216)μg/mol肌酐]明顯高于BOO 2週組[(131 ±49)μg/mol肌酐]和對照組[(107 ±22)μg/mol肌酐,均P＜0.05].結論 逼尿肌bFGF mRNA錶達隨BOO進展持續升高,TGFβ1 mRNA在失代償階段錶達纔明顯升高;尿液TGFβ1在大鼠BOO 6週時呈彊錶達,有可能成為預測BOO後逼尿肌收縮功能的指標.
목적 탐토전화생장인자β1(TGFβ1)、감성성섬유세포생장인자(bFGF)여대서방광출구경조(BOO)후핍뇨기수축공능장애적관계.방법 건립Wistar대서BOO모형(BOO 2주조11지;BOO 6주조10지),가수술조(8지)작위대조,측정리체핍뇨기기조M수체격동제자격하수축력,RT-PCR방법측정핍뇨기중TGFB1 mRNA、bFGF mRNA적표체,ELISA측정뇨액중TGFβ1、bFGF 적수평.결과 재1×10-4、1×10-3 mmo/L록화안기갑선담감(Carbachol)농도하BOO 2주조적최대핍뇨기수축력[(0.96±0.11)、(1.98±0.21)g]대우대조조[(0.85±0.18)、(1.82 ±0.19)g,균P＜0.05];대서재1×10-5、1×10-4、1×10-3화1 × 10-2 mmol/L Carbachol농도하BOO 6주조적최대핍뇨기수축력[(0.19 ±0.02)、(0.65 ±0.06)、(1.12 ±0.08)화(1.40 ±0.19)g]균소우BOO 2주조[(0.24±0.03)、(0.96 ±0.11)、(1.98 ±0.21)화(2.16 ±0.21)g,균P＜0.05]화대조조[(0.23 ±0.04)、(0.85 ±0.18)、(1.82 ±0.19)화(2.12 ±0.26)g,균P＜0.05].TGFβ1 mRNA재대조조、BOO 2주조、BOO 6주조핍뇨기적표체분별위0.32 ±0.01、0.34 ±0.10화0.72 ±0.21,후량조지간차이유통계학의의(P＜0.01);bFGF mRNA표체분별위0.10±0.05、0.21±0.07화0.38 ± 0.13,조간량량비교,차이균유통계학의의(균P＜0.05).리체핍뇨기수축력여기TGFβ1 mRNA、bFGF mRNA표체수평정부상관(균P＜0.05).BOO 6주조뇨중TGFβ1표체[(606±216)μg/mol기항]명현고우BOO 2주조[(131 ±49)μg/mol기항]화대조조[(107 ±22)μg/mol기항,균P＜0.05].결론 핍뇨기bFGF mRNA표체수BOO진전지속승고,TGFβ1 mRNA재실대상계단표체재명현승고;뇨액TGFβ1재대서BOO 6주시정강표체,유가능성위예측BOO후핍뇨기수축공능적지표.
Objective To investigate the relationship of transforming growth factor β1(TGFβ1)and basic fibroblast growth factor(bFGF)to detrusor underactivity following bladder outlet obstruction (BOO).Methods Female Wistar rats underwent ligation of the urethra to establish BOO models and were divided into BOO model 2-week group(11 rats)and BOO model 6-week group(10 rats).8 rats underwent sham operation as control group.The detrusor urinae was taken out and stimulated by carbachol to measure the detrusor contraction force(DCF).RT-PCR method was employed to measure the mRNA expression of TGF β1 and bFGF in the detrusor urinae.Urine TGFβ1 and bFGF were determined by ELISA.Results The maximum DCF levels of the BOO 2-week group under the 1×10-4 mmol/L and 1×10-3 mmol/L carbachol concentrations were 0.96 g ±0.11 g and 1.98 g ±0.21 g respectively, both significantly higher than those of the sham operation group(0.85 g±0.18 g and 1.82 g ±0.19 g respectively, both P＜0.05).The maximum DCF levels of the BOO 6-week group under the 1×10-3,1×10-4,1×10-3 and 1×10-2 mmol/L carbachol concentrations were 0.19 g ±0.02 g,0.65 g±0.06 g,1.12 g±0.08 g,and 1.40 g±0.19 g respectively,all significantly lower than those of the BOO 2-week group(0.24 g ±0.03 g,0.96 g±0.11 g,1.98 g±0.21 g,and 2.16 g±0.21 g respectively,all P ＜0.05)and those of the sham operation group(0.23 g±0.04 g,0.85 g±0.18 g,1.82 g±0.19 g,and 2.12 g±0.26 g respectively,all P＜0.05).The mRNA expression of TGFβ1 of the BOO 6-week group, BOO 2-week group,and sham operation group was 0.72±0.21,0.34±0.10,and 0.32±0.01 respectively, there was a significant difference between the BOO 6-week group and the BOO 2-week group(P＜0.01).The mRNA expression level of bFGF of the BOO 6-wwek group was 0.38±0.13,significanfly higher than tllose of the BOO 2-week group and sham operation group(0.21±0.07 and 0.10±0.05 respectively, both P＜0.05).DCF was negatively correlated with the mNRA expression of TGFβ1 and the mNRA expression bFGF in detrusor (both P＜0.05).The urine TGFβ1 of the BOO 6-week group was(606±216)μg/mol Cr,significantly higher than that of the BOO 2-week group[(131±49)μg/mol Cr]and that of the sham operation group[(107±22)μg/mol Cr,both P＜0.05].Conclusion With the progres8ion of BOO,there is a sustained rise of bFGF mRNA expression in detrusor;however,the TGFβ1 mRNA expression only increases during the decompensation stage.Urine TGFβ1 level is very high 6 weeks after BOO,which may help predict the contraction function of bladder after BOO.
