CAJ | 학술논문

目的探讨核心蛋白聚糖(DCN)基因对S180荷瘤小鼠免疫功能的影响及其抗肿瘤作用.方法昆明小鼠24只,每组8只,建立S180实体移植瘤模型,随机分为生理盐水组(阴性对照组)、pcDNA3.1(+)空载体组、pcDNA3.1(+)-DCN转基因治疗组,于接种瘤细胞第2天瘤内注射用药,第5天起隔天测量小鼠肿瘤体积,并采用MTF法检测T淋巴细胞转化能力、RT-PCR检测TGF-β mRNA转录水平、ELISA测定血清VEGF的变化.结果与前两组相比,重组载体组肿瘤体积缩小为(2.219±0.105)cm3(P＜0.001);淋巴细胞增生率提高为(67.21±6.32)%(P＜0.01,P＜0.05);同时使TGF-β表达降低,条带变淡;VEGF表达也明显下调,其A值降为2.6175±0.099(P＜0.01,P＜0.05).结论肿瘤组织内DCN能够显著抑制肿瘤细胞生长,提高荷瘤小鼠免疫系统的活性.
목적 탐토핵심단백취당(DCN)기인대S180하류소서면역공능적영향급기항종류작용.방법 곤명소서24지,매조8지,건립S180실체이식류모형,수궤분위생리염수조(음성대조조)、pcDNA3.1(+)공재체조、pcDNA3.1(+)-DCN전기인치료조,우접충류세포제2천류내주사용약,제5천기격천측량소서종류체적,병채용MTF법검측T림파세포전화능력、RT-PCR검측TGF-β mRNA전록수평、ELISA측정혈청VEGF적변화.결과 여전량조상비,중조재체조종류체적축소위(2.219±0.105)cm3(P＜0.001);림파세포증생솔제고위(67.21±6.32)%(P＜0.01,P＜0.05);동시사TGF-β표체강저,조대변담;VEGF표체야명현하조,기A치강위2.6175±0.099(P＜0.01,P＜0.05).결론 종류조직내DCN능구현저억제종류세포생장,제고하류소서면역계통적활성.
Objective To investigate the anti-tumorigenesis function of DCN gene and its effect on the immunological funotions of mice bearing S180.Methods Twenty four mice bearing S180 were divided into three groups at random(eight mice every group),and then the normal saline group(negative control),pcDNA3.1(+)vector group and pcDNA3.1(+)-DCN group were set up for the experiment.They all started to inject next day by armpit.The volume of tumors were measured at the 5th day,7th day,9th day,11th day.The transformation rate of T lymphocytes were detected by means of MTT,the mRNA level of TGF-β by RT-PCR and the level of VEGF in serum by ELISA.Results The average volume of tumor in pcDNA3.1 (+)-DCN group were deflated to(2.219±0.105)cm3 comparing with the others(P＜0.001);the transformation rate of T lymphocytes were increased significantly to(67.21±6.32)%(P＜0.01,P＜0.05);meanwhile it can decrease the mRNA level of TGF-β in serum,and depress the expression of VEGF markedly,whose absorbance were2.6175±0.099(P＜0,01,P＜0.05).Conclusion DCN gene could significantly inhibit the growth of tumor,and enhance the immunological function of mice bearing S180 in vivo.