国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2008年
2期
87-89
,共3页
韩兆东%阮月芹%张兰%樊其忠%傅廷亮
韓兆東%阮月芹%張蘭%樊其忠%傅廷亮
한조동%원월근%장란%번기충%부정량
黄芪%梗阻性黄疸%细胞免疫%幼鼠
黃芪%梗阻性黃疸%細胞免疫%幼鼠
황기%경조성황달%세포면역%유서
Astragalus%Obstructive jaundice%Cellular immunity%Immature rats
目的 探讨黄芪对梗阻性黄疸幼鼠血液和肠黏膜T淋巴细胞免疫功能的影响.方法 以3周龄Wistar幼鼠为载体,随机分为4组:正常对照组、假手术组、梗阻性黄疸(OJ)组、OJ+黄芪组(OJ+A),每组各12只.利用流式细胞仪检测实验幼鼠外周血及肠黏膜T淋巴细胞(CD4、CD8)的百分率,计算CD4/CD8比值.结果 OJ组外周血和肠黏膜CD4细胞、CD4/CD8比值明显低于正常对照组及假手术组,有显著性差异(P＜0.05).OJ+A组外周血和肠黏膜CD4细胞、CD4/CD8比值均较OJ组升高,有显著性差异(P＜0.05).结论 黄芪能提高OJ幼鼠T淋巴细胞的免疫功能.
目的 探討黃芪對梗阻性黃疸幼鼠血液和腸黏膜T淋巴細胞免疫功能的影響.方法 以3週齡Wistar幼鼠為載體,隨機分為4組:正常對照組、假手術組、梗阻性黃疸(OJ)組、OJ+黃芪組(OJ+A),每組各12隻.利用流式細胞儀檢測實驗幼鼠外週血及腸黏膜T淋巴細胞(CD4、CD8)的百分率,計算CD4/CD8比值.結果 OJ組外週血和腸黏膜CD4細胞、CD4/CD8比值明顯低于正常對照組及假手術組,有顯著性差異(P＜0.05).OJ+A組外週血和腸黏膜CD4細胞、CD4/CD8比值均較OJ組升高,有顯著性差異(P＜0.05).結論 黃芪能提高OJ幼鼠T淋巴細胞的免疫功能.
목적 탐토황기대경조성황달유서혈액화장점막T림파세포면역공능적영향.방법 이3주령Wistar유서위재체,수궤분위4조:정상대조조、가수술조、경조성황달(OJ)조、OJ+황기조(OJ+A),매조각12지.이용류식세포의검측실험유서외주혈급장점막T림파세포(CD4、CD8)적백분솔,계산CD4/CD8비치.결과 OJ조외주혈화장점막CD4세포、CD4/CD8비치명현저우정상대조조급가수술조,유현저성차이(P＜0.05).OJ+A조외주혈화장점막CD4세포、CD4/CD8비치균교OJ조승고,유현저성차이(P＜0.05).결론 황기능제고OJ유서T림파세포적면역공능.
Objective To evaluate the effect of Astragalus on T lymphocyte immune function in peripheral blood and intestinal mucosa of immature rats with obstructive jaundice. Methods Three-week-old Wistar rats were randomly divided into four groups(n=12,in each):control group, sham operation group, obstructive jaundice(OJ)group,OJ+Astragalus (OJ+A)group.The percentage of CD4 and CD8 T lymphocytes, the ratio of CD4 to CD8 in peripheral blood and intestinal mucosa of immature rats was detected by flow cytometry. Result The percentage of CD4+ cell and the ratio of CD4 to CD8 in the OJ group were lower than those in the control group and the sham operation group,with significant difference(P＜0.05).The percentage of CD4 cell and the ratio of CD4 to CD8 in the OJ+A group were higher than those in the OJ group(P＜0.05).Conclusion Astragalus can increase T lymphocyte immune function in OJ immature rats.
