CAJ | 학술논문

目的探讨急性白血病( AL) p27基因mRNA的表达及临床意义.方法采用反转录聚合酶链反应( RT-PCR)检测65例初治AL和41例造血系统非恶性肿瘤患者(对照组)骨髓单个核细胞中p27基因mRNA表达水平,分析p27 mRNA表达与AL患者的年龄、性别、外周血白细胞计数和髓外浸润临床特征的相关性.结果 AL中p27基因mRNA表达的阳性率为36.9％(24/65),显著低于对照组的87.8％(36/41),差异有统计学意义(P＜0.05),急性髓系白血病(AML)组和急性淋巴细胞白血病( ALL)组分别为34.2％(13/38)和37.5％(9/24),均低于对照组,差异有统计学意义(均P＜0.05),但AML组和ALL组比较,差异无统计学意义(P＞0.05).p27 mRNA表达年龄≥14岁组(52例)和＜14岁组(13例)比较,差异无统计学意义(P＞0.05)；男性组(34例)和女性组(31例)比较,差异无统计学意义(P＞0.05)；外周血白细胞计数≥20×109/L组(31例)和＜20×109/L组(34例)比较,p27mRNA表达与白细胞计数呈负相关(r=-0.284,P＜ 0.05)；有髓外浸润组(37例)和无髓外浸润组(28例)比较,p27 mRNA表达与髓外浸润呈负相关(r=-0.300,P＜0.05).结论 AL存在p27基因mRNA的表达缺失,缺失同时存在于AML和ALL中,缺失与患者外周血白细胞计数、髓外浸润呈负相关,这可能是AL发生、发展的机制之一.
목적 탐토급성백혈병( AL) p27기인mRNA적표체급림상의의.방법 채용반전록취합매련반응( RT-PCR)검측65례초치AL화41례조혈계통비악성종류환자(대조조)골수단개핵세포중p27기인mRNA표체수평,분석p27 mRNA표체여AL환자적년령、성별、외주혈백세포계수화수외침윤림상특정적상관성.결과 AL중p27기인mRNA표체적양성솔위36.9％(24/65),현저저우대조조적87.8％(36/41),차이유통계학의의(P＜0.05),급성수계백혈병(AML)조화급성림파세포백혈병( ALL)조분별위34.2％(13/38)화37.5％(9/24),균저우대조조,차이유통계학의의(균P＜0.05),단AML조화ALL조비교,차이무통계학의의(P＞0.05).p27 mRNA표체년령≥14세조(52례)화＜14세조(13례)비교,차이무통계학의의(P＞0.05)；남성조(34례)화녀성조(31례)비교,차이무통계학의의(P＞0.05)；외주혈백세포계수≥20×109/L조(31례)화＜20×109/L조(34례)비교,p27mRNA표체여백세포계수정부상관(r=-0.284,P＜ 0.05)；유수외침윤조(37례)화무수외침윤조(28례)비교,p27 mRNA표체여수외침윤정부상관(r=-0.300,P＜0.05).결론 AL존재p27기인mRNA적표체결실,결실동시존재우AML화ALL중,결실여환자외주혈백세포계수、수외침윤정부상관,저가능시AL발생、발전적궤제지일.
Objective To explore the p27 gene expression and clinical significance in acute leukemia (AL).Methods RT-PCR was used for testing the mRNA levels of p27 gene in bone marrow mononuclear cell of 65 cases of newly diagnosed AL patients and 41 controls.The correlation between the p27 gene expression positive rate and age, sex, peripheral blood white blood cell count and extramedullary infiltration were analyzed simultaneously in AL patients.Results The positive rate of p27 gene expression (36.9 ％,24/65)was significantly lower in AL group than that in the control group (87.8 ％,36/41) (P ＜0.05),and which was lower than the control group (P ＜0.05) in the AML group (34.2 ％,13/38) and the ALL group (37.5 ％,9/24).However,comparing AML group with ALL group,the difference was not statistically significant (P ＞0.05).At the same time,compared the p27 gene expression positive rate of ≥ 14 years old group (52 cases) and ＜14 years old group (13 cases),and also compared in male group (34 cases) and in female group (31 cases),there was no statistically difference (P ＞0.05); Compared the p27 gene expression positive rate of peripheral blood white blood cell count ≥20×109/L group (31 cases) and ＜20×109/L group (34 cases),also compared with in extramedullary infiltration group (37 cases) and in no extramedullary infiltration group (28 cases),the relationship of p27 gene expression positive rate with peripheral blood white blood cell count as well as extramedullary infiltration was a negative correlation (r =-0.284,P ＜ 0.05,r =-0.300,P ＜ 0.05).Conclusion There is p27 mRNA expression deletion in AL patients,and the deletion is present in the AML and ALL at the same time.p27 mRNA expression is negatively correlated with peripheral blood white blood cell count or extramedullary infiltration,which might be one of the mechanisms of occurrence and development of AL.