CAJ | 학술논문

目的研究Rho GDP解离抑制因子(Rho GDP dissociation inhibitor 2,RhoGDI2)mRNA 及Bcl-2 mRNA的表达在缺氧缺血性脑损伤(hypoxic-ischemic brain damage,HIBD)中的作用和机制.方法 30只新生7日龄SD大鼠按照完全随机化方法分为假手术组及HIBD 6 h和48 h组,每组10只.采用流式细胞仪检测脑细胞凋亡情况,并用Real-time RT-PCR方法测定脑组织中RhoGDI2和Bcl-2mRNA表达水平.结果 (1)新生大鼠HIBD后48h结扎侧大脑半球脑水肿明显.(2) HIBD6 h出现典型的凋亡细胞峰,细胞凋亡率为(1.40±0.12)％.HIBD 48 h凋亡峰更为明显,细胞凋亡率达到( 15.86±0.98)％.缺氧缺血后与假手术组相比,差异有统计学意义(P＜0.01).(3)假手术组大鼠RhoGDI2和Bcl-2 mRNA表达水平较高(4.12±0.74、2.55±0.65),在HIBD 6 h后二者表达开始下降(3.19±0.77、1.96±0.36),48 h降低更加明显(1.04±0.18、1.06±0.17),与假手术组比较,HIBD各时间点RhoGDI2和Bcl-2 mRNA的表达均明显降低,差异有统计学意义(P＜0.01).(4)缺氧缺m后各时间点RhoGDI2 mRNA的表达和Bcl-2 mRNA的表达呈正相关(r=0.831,P＜0.05).结论脑缺氧缺血后,随着凋亡的出现,RhoGDI2和Bcl-2 mRNA表达水平降低,提示Rh0GDI2表达失衡可能通过Bcl-2参与新生大鼠HIBD中凋亡的发生.
목적 연구Rho GDP해리억제인자(Rho GDP dissociation inhibitor 2,RhoGDI2)mRNA 급Bcl-2 mRNA적표체재결양결혈성뇌손상(hypoxic-ischemic brain damage,HIBD)중적작용화궤제.방법 30지신생7일령SD대서안조완전수궤화방법분위가수술조급HIBD 6 h화48 h조,매조10지.채용류식세포의검측뇌세포조망정황,병용Real-time RT-PCR방법측정뇌조직중RhoGDI2화Bcl-2mRNA표체수평.결과 (1)신생대서HIBD후48h결찰측대뇌반구뇌수종명현.(2) HIBD6 h출현전형적조망세포봉,세포조망솔위(1.40±0.12)％.HIBD 48 h조망봉경위명현,세포조망솔체도( 15.86±0.98)％.결양결혈후여가수술조상비,차이유통계학의의(P＜0.01).(3)가수술조대서RhoGDI2화Bcl-2 mRNA표체수평교고(4.12±0.74、2.55±0.65),재HIBD 6 h후이자표체개시하강(3.19±0.77、1.96±0.36),48 h강저경가명현(1.04±0.18、1.06±0.17),여가수술조비교,HIBD각시간점RhoGDI2화Bcl-2 mRNA적표체균명현강저,차이유통계학의의(P＜0.01).(4)결양결m후각시간점RhoGDI2 mRNA적표체화Bcl-2 mRNA적표체정정상관(r=0.831,P＜0.05).결론 뇌결양결혈후,수착조망적출현,RhoGDI2화Bcl-2 mRNA표체수평강저,제시Rh0GDI2표체실형가능통과Bcl-2삼여신생대서HIBD중조망적발생.
Objective To investigate the effect of Rho GDP dissociation inhibitor 2 (RhoGDI2) and Bcl-2 in pathogenesis of hypoxic-ischemic brain damage (HIBD).Methods Neonatal 7-day-old Sprague Dawley rats were randomly divided into sham-operation control group,HIBD 6 h group and HIBD 48 h group (n =10 per group).The apoptosis rate of brain cell was measured by flow cytometer and the expression of RhoGDI2 mRNA and Bcl-2 mRNA were detected by Real-time RT-PCR.Results ( 1 ) The ligated cerebral hemisphere of neonatal rats showed obvious edema at 48 h after hypoxia-ischemia.( 2 ) Apoptotic cell appeared at 6 h in HIBD group,the apoptosis rate was ( 1.40 ± 0.12 ) ％.The apoptosis rate obviously increased to (15.86 ±0.98)％ at 48 h after HIBD,which showed a significant increase compared to sham-operation control group ( P ＜ 0.01 ).( 3 ) The expressions of both RhoGDI2 mRNA and Bcl-2 mRNA were 4.12 ±0.74 and 2.55 ± 0.65 respectively in sham-operation control group.In HIBD group,the expressions of both RhoGDI2 mRNA and Bcl-2 mRNA began to decrease at 6 h after HIBD ( 3.19 ± 0.77,1.96 ± 0.36) and decreased furthermore at 48 h after HIBD ( 1.04 ±0.18,1.06 ±0.17 ).The differences of expression levels among three groups were statistically significant (P ＜0.01 ).(4) The expression of RhoGDI2 mRNA positively correlated with the expression of Bcl-2 mRNA ( r =0.831,P ＜ 0.05 ).Conclusion With the emerging of apoptosis after HIBD,the expressions of both RhoGDI2 mRNA and Bcl-2 mRNA are decreased.The imbalance of expression of RhoGDI2 is involved in pathogenesis of HIBD by regulating Bcl-2 expression.