CAJ | 학술논문

目的分析Bmi-1基因在甲状腺疾病组织中的表达,探讨Bmi-1与甲状腺乳头状癌转移的相关关系及临床意义.方法采用RT-PCR法检测8例正常甲状腺、18例结节性甲状腺肿、24例甲状腺乳头状癌组织中Bmi-1基因的表达,运用Fisher确切概率法分别对正常组、结甲组和甲癌组检验,判断Bmi-1在各组中的表达.结果①Bmi-1基因阳性表达在正常甲状腺中仅1例(12.5%),在结节性甲状腺肿中有3例(16.7%),而在甲状腺癌中有19例(79.2%),3组阳性率比较差异有统计学意义(P<0.05);②在24例甲状腺癌中,有淋巴结转移的16例(66.67%),无淋巴结转移的8例(33.33%),2组间比较差异有统计学意义(P<0.05);③以年龄为界,在19例阳性表达的甲状腺癌中,年龄≥45岁的12例(63.16%),<45岁的7例(36.8%),2组间比较差异有统计学意义(P<0.05).结论 ①研究证实Bmi-1基因在甲状腺癌中的阳性率明显高于良性甲状腺疾病和正常甲状腺组织;②Bmi-1的表达与淋巴结的转移及年龄有显著相关性,提示它对判断甲状腺乳头状癌的转移机制及预后有一定的临床价值.
목적 분석Bmi-1기인재갑상선질병조직중적표체,탐토Bmi-1여갑상선유두상암전이적상관관계급림상의의.방법 채용RT-PCR법검측8례정상갑상선、18례결절성갑상선종、24례갑상선유두상암조직중Bmi-1기인적표체,운용Fisher학절개솔법분별대정상조、결갑조화갑암조검험,판단Bmi-1재각조중적표체.결과①Bmi-1기인양성표체재정상갑상선중부1례(12.5%),재결절성갑상선종중유3례(16.7%),이재갑상선암중유19례(79.2%),3조양성솔비교차이유통계학의의(P<0.05);②재24례갑상선암중,유림파결전이적16례(66.67%),무림파결전이적8례(33.33%),2조간비교차이유통계학의의(P<0.05);③이년령위계,재19례양성표체적갑상선암중,년령≥45세적12례(63.16%),<45세적7례(36.8%),2조간비교차이유통계학의의(P<0.05).결론 ①연구증실Bmi-1기인재갑상선암중적양성솔명현고우량성갑상선질병화정상갑상선조직;②Bmi-1적표체여림파결적전이급년령유현저상관성,제시타대판단갑상선유두상암적전이궤제급예후유일정적림상개치.
Objective To analyze expression of Bmi-1 gene in thyroid tissues and the relationship between Bmi-1 gene and metastasis of papillary thyroid carcinoma.Methods RT-PCR was used to detect expression of Bmi-1 in normal thyrroid tissues(8 eases),nodular goiters(18 cases)and papillary thyroid carcinomas (24 cases).The result was analyzed by Fisher exact propability test.Results Bmi-1 expression was detected only in 1 case(12.5%)in normal thyroid tissues group,3 cases(16.7%)in nodular goiters group,and 19 cases(79.2%)in papillary thyroid carcinoma group.The difference of Bmi-1 expression between the 3 groups had statistical significance(P<0.05).There were 16 cases of lymph node metastasis(66.67%)in the 24 cases of papillary thyroid carcinoma.The difference of Bmi-1 expression between cases with cervical lymph node metastasis and those without metastasis had statistical significance(P<0.05).Among the 19 thyroid carcinoma cases in which Bmi-1 expression was positive,12 eases(63.2%)were over45 years old,and 7 cases(36.8%)were under 45 years.The difference had statistical significance(P<0.05).Conclusions Expression of Bmi-1 gene in papillary thyroid carcinoma tissue is significantly higher than that in nodular goiters and normal thyroid tissues.Bmi-1 expression is closely relevant to lymph node metastasis and age,implying that Bmi-1 expression has clinical significance of distinguishing thyroid malignant tumors from benign diseases and it may provide clues to unravel the mechanism and prognosis of papillary thyroid carcinoma metastasis.