CAJ | 학술논문

目的在大鼠小肠缺血/再灌注(I/R)的不同时期应用高压氧(HBO)干预治疗,观察其对大鼠肠黏膜的保护作用.方法采用肠系膜上动脉夹闭/松夹闭方式建立小肠I/R损伤模型.50只大鼠随机分为5组(每组10只):对照组(sham组)、I/R组、HBO预处理组(HBO-P组)、缺血期RBO组(HBO-I组)和再灌注期HBO组(HBO-R组).采用酶联免疫吸附试验法(ELISA)检测肠道组织肿瘤坏死因子-α(TNF-α);应用翻转囊袋法测定胰岛素(相对分子质量5000)黏膜-浆膜通过量以检测黏膜通透性;应用光镜和电镜观察肠道组织学改变并进行损伤评分.结果肠黏膜通透性依次为:Sham组最低,其次HBO-I组,再次HBO-P组(每2组比较P<0.05),均明显低于HBO-R组和I/B组(P<0.05);HBO-R组略低于I/R组,但2组比较差异无统计学意义(P>0.05).肠道组织TNF-α含量依次是:Sham组最低,其次HBO-I组,再次HBO-P组(每2组比较P<0.05),均明显低于HBO-R组和I/B组(P<0.05);HBO-R组略低于I/R组,但2组比较差异无统计学意义(P>0.05).组织学损伤评分依次是:Sham组最低,其次为HBO-I组,HBO-P组次之(每2组比较P<0.05),均明显轻于HBO-R组和I/R组(P<0.05);HBO-R组略轻于I/R组,但2组比较差异无统计学意义(P>0.05).结论 HBO预处理和缺血期应用HBO对大鼠肠黏膜I/R损伤有明显的保护作用,可以降低肠黏膜通透性和减少炎症反应;再灌注期应用HBO虽然对肠黏膜有一定的保护作用,但效果不明显.
목적 재대서소장결혈/재관주(I/R)적불동시기응용고압양(HBO)간예치료,관찰기대대서장점막적보호작용.방법 채용장계막상동맥협폐/송협폐방식건립소장I/R손상모형.50지대서수궤분위5조(매조10지):대조조(sham조)、I/R조、HBO예처리조(HBO-P조)、결혈기RBO조(HBO-I조)화재관주기HBO조(HBO-R조).채용매련면역흡부시험법(ELISA)검측장도조직종류배사인자-α(TNF-α);응용번전낭대법측정이도소(상대분자질량5000)점막-장막통과량이검측점막통투성;응용광경화전경관찰장도조직학개변병진행손상평분.결과 장점막통투성의차위:Sham조최저,기차HBO-I조,재차HBO-P조(매2조비교P<0.05),균명현저우HBO-R조화I/B조(P<0.05);HBO-R조략저우I/R조,단2조비교차이무통계학의의(P>0.05).장도조직TNF-α함량의차시:Sham조최저,기차HBO-I조,재차HBO-P조(매2조비교P<0.05),균명현저우HBO-R조화I/B조(P<0.05);HBO-R조략저우I/R조,단2조비교차이무통계학의의(P>0.05).조직학손상평분의차시:Sham조최저,기차위HBO-I조,HBO-P조차지(매2조비교P<0.05),균명현경우HBO-R조화I/R조(P<0.05);HBO-R조략경우I/R조,단2조비교차이무통계학의의(P>0.05).결론 HBO예처리화결혈기응용HBO대대서장점막I/R손상유명현적보호작용,가이강저장점막통투성화감소염증반응;재관주기응용HBO수연대장점막유일정적보호작용,단효과불명현.
Objective To investigate protective effects of hyperbaric oxygen (HBO) on small intestine mucous membrane of SD rats during different stages of ischemia reperfusion (I/R). Methods The animal model of small intestine ischemia reperfusion was developed by clamping the superior mesenteric artery (SMA) for 60 min. Fifty male SD rats were randomly divided into 5 groups (n=10), i.e. the sham group, the I/R group, the HBO pretreated group before ischemia (HBO-P), the HBO treatment group during ischemia (HBO-I), and the HBO treatment group during reperfusion (HBO-R). Levels of TNF-α in the intestinal tissue were measured by using enzyme -linked immunosorbent assay method (ELISA). To assess intestinal mucosal barrier function, inverted gut sacs were prepared to measure the mucosal-to-serosal passage of insulin (molecular weight is 5000). Intestinal histological changes were observed by using optical microscope and electron microscope. Injury scores were made for further assessment. Results Mucosal permeability of the intestine following ischemia reperfusion came in the following order: the sham group being the lowest, next the HBO-I group, and then the HBO-P group. Differences could be seen, when comparisons were made between the two groups (P>0.05). TNF-α levels in the intestine were in the following succession: the sham group being the lowest, next the HBO-I group, and then the HBO-P group (P <0.05). However, levels of TNF-α in the first two groups were obviously lower than those of the HBO-R and I/R groups (P < 0.05). And TNF-α level of the HBO-R group was slightly lower than that of I/R group, but without statistical difference (P >0.05). Histological injury scores of the intestinal tissue in HBO-I were significantly lower than those in the HBO-P group (P <0.05), when a comparison was made between the HBO-R and I/R groups (P < 0.05), without significant statistical differences between the HBO-R and I/R groups (P > 0.05). Conclusions HBO pre-treatment and HBO therapy during ischemia seemed to have obvious protective effect on the injury of small intestinal mucosa induced by ischemia reperfusion, could decrease mucosal permeability and reduce inflammatory response. Though HBO therapy during reperfusion had certain protective effect on intestinal mucosa, no obvious results were noted, and no statistical differences could be seen when it was compared with the I/R Group.