CAJ | 학술논문

目的探讨CD147和基质金属蛋白酶9(MMP-9)在非霍奇金淋巴瘤(NHL)中的表达,分析表达水平与临床分期、肿块大小、血清乳酸脱氢酶(LDH)水平及与预后的关系.方法采用免疫组化方法研究病理标本中CD147、MMP-9的表达及其与临床指标之间的关系,探讨两者的相关性及其与NHL预后的关系.结果 ①免疫组化结果 :73%病例(45/62)CD147表达阳性,其中(+)11例,(++)13例,(+++)21例,阴性表达17例;81%病例(50/62)MMP-9表达阳性,其中(+)13例,(++)18例,(+++)19例,阴性表达12例;CD147与MMP-9的表达有明显相关性.②CD147的表达与临床骨髓浸润、肿块大小、LDH值以及临床分期有关;MMP-9的表达与骨髓浸润和临床分期有关.③CD147(-)～(+)组5年生存率明显高于(++)～(+++)组,同样MMP-9(-)～(+)组5年存活率显著高于(++)～(+++)组.Cox多因素风险分析提示CD147和MMP-9都是影响预后的重要因素.结论 CD147和MMP-9的表达与NHL的预后相关,高表达者预后不良.
목적 탐토CD147화기질금속단백매9(MMP-9)재비곽기금림파류(NHL)중적표체,분석표체수평여림상분기、종괴대소、혈청유산탈경매(LDH)수평급여예후적관계.방법채용면역조화방법연구병리표본중CD147、MMP-9적표체급기여림상지표지간적관계,탐토량자적상관성급기여NHL예후적관계.결과 ①면역조화결과 :73%병례(45/62)CD147표체양성,기중(+)11례,(++)13례,(+++)21례,음성표체17례;81%병례(50/62)MMP-9표체양성,기중(+)13례,(++)18례,(+++)19례,음성표체12례;CD147여MMP-9적표체유명현상관성.②CD147적표체여림상골수침윤、종괴대소、LDH치이급림상분기유관;MMP-9적표체여골수침윤화림상분기유관.③CD147(-)～(+)조5년생존솔명현고우(++)～(+++)조,동양MMP-9(-)～(+)조5년존활솔현저고우(++)～(+++)조.Cox다인소풍험분석제시CD147화MMP-9도시영향예후적중요인소.결론 CD147화MMP-9적표체여NHL적예후상관,고표체자예후불량.
Objective To investigate the expression of CD147 and matrix metalloproteinase-9 (MMP-9) in children with non-Hodgkin's lymphorna (NHL) and its correlation with clinical stage, tumor size, bone marrow invasion, immunological typing, serum lactate dehydrogenase (LDH) concentration, and prognosis. Methods Specimens excised from NHL patients were prepared. Expression of CD147 and MMP-9 were tested by streptavidin-biotin complex (SABC) immunohistochemistry and its correlation with clinical results were analyzed. Results The positive rate of CD147 expression was 73% (45/62), 17 cases were (-), 11 cases (+), 34 cases (++) and 21 cases (+++). The positive rate of MMP-9 expression was 81% (50/62), 12 cases were (-), 13 cases (+), 18 cases (++) and 19 cases (+++). The Spearman rank correlation analysis indicated that there was a positive correlation between CD147 and MMP-9 expressions in NHL (r_s=0.763, P=0.034). Expression of CD147 was determined in relation to factors that included clinical bone marrow invasion, tumor size, LDH level as well as the clinical stage; expression of MMP-9 had a positive correlation with bone marrow invasion, tumor size and clinical phases. The 5-year survival rates (5YSR) were 78% (22/28) and 45% (15/34) in the eases whose CD147 expression was (-)-(+) and (++)-(+++), respectively, and 5YSR were 84% (21/25) and 43% (16/37) in the cases whose MMP-9 expression was (-)-(+) and (++)-(+++) respectively, the difference was significant. Cox multivariate analysis showed that both CD147 and MMP-9 were important prognostic factors. Conclusion The increased expression of CD147 and/or MMP-9 correlates with a poor clinical outcome in patients with NHL.