中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2009年
6期
380-383
,共4页
杨骥%杨雪%张菊莉%储以微%朱鹭冰%高地%李明
楊驥%楊雪%張菊莉%儲以微%硃鷺冰%高地%李明
양기%양설%장국리%저이미%주로빙%고지%리명
红斑狼疮,系统性%Th17%细胞因子类
紅斑狼瘡,繫統性%Th17%細胞因子類
홍반랑창,계통성%Th17%세포인자류
Lupus erythematosus,systemic%Th17 cells%Cytokines
目的 探讨产生IL-17的CD4+T细胞(Th17)在SLE患者组织中的定位,以及与狼疮活动的关系.方法 共聚焦显微镜、免疫荧光双标技术、免疫组化和HE染色,分析Th17细胞业群在4例活动期SLE患者和2例正常人外周血单一核细胞中、皮损组织和肺组织中的定位.流式细胞仪检测50例SLE患者和15例正常人对照外周血Th17的比例,RT-PCR检测相关细胞因子基因表达,用ELISA检测血清中IL-17的分泌水平.结果 在活动期狼疮患者外周血单一核细胞中可见有Th17细胞,其IL-17的荧光强度为(127.6±20.5),明显高于正常人IL-17荧光强度,其值为(40.6±11.1),P＜0.001.在活动期SLE患者受损的皮肤组织和肺组织中可见有Th17细胞的浸润,而正常人皮肤未见有Th17细胞的浸润.活动期SLE患者外周血中Th17比例明显增加,且与SLE活动指数(SLEDAI)呈正相关.相关细胞因子IL-17A和IL-17F mRNA表达明显增加,血清中的不IL-17水平分泌增加,活动期SLE患者中Th17增加与血管炎的发病呈正相关,经过治疗后Th17随病情缓减而减少.结论 Th17细胞亚群在活动期SLE患者体内扩增,其扩增与SLE活动密切相关,可能参与SLE 血管炎的发生.
目的 探討產生IL-17的CD4+T細胞(Th17)在SLE患者組織中的定位,以及與狼瘡活動的關繫.方法 共聚焦顯微鏡、免疫熒光雙標技術、免疫組化和HE染色,分析Th17細胞業群在4例活動期SLE患者和2例正常人外週血單一覈細胞中、皮損組織和肺組織中的定位.流式細胞儀檢測50例SLE患者和15例正常人對照外週血Th17的比例,RT-PCR檢測相關細胞因子基因錶達,用ELISA檢測血清中IL-17的分泌水平.結果 在活動期狼瘡患者外週血單一覈細胞中可見有Th17細胞,其IL-17的熒光彊度為(127.6±20.5),明顯高于正常人IL-17熒光彊度,其值為(40.6±11.1),P＜0.001.在活動期SLE患者受損的皮膚組織和肺組織中可見有Th17細胞的浸潤,而正常人皮膚未見有Th17細胞的浸潤.活動期SLE患者外週血中Th17比例明顯增加,且與SLE活動指數(SLEDAI)呈正相關.相關細胞因子IL-17A和IL-17F mRNA錶達明顯增加,血清中的不IL-17水平分泌增加,活動期SLE患者中Th17增加與血管炎的髮病呈正相關,經過治療後Th17隨病情緩減而減少.結論 Th17細胞亞群在活動期SLE患者體內擴增,其擴增與SLE活動密切相關,可能參與SLE 血管炎的髮生.
목적 탐토산생IL-17적CD4+T세포(Th17)재SLE환자조직중적정위,이급여랑창활동적관계.방법 공취초현미경、면역형광쌍표기술、면역조화화HE염색,분석Th17세포업군재4례활동기SLE환자화2례정상인외주혈단일핵세포중、피손조직화폐조직중적정위.류식세포의검측50례SLE환자화15례정상인대조외주혈Th17적비례,RT-PCR검측상관세포인자기인표체,용ELISA검측혈청중IL-17적분비수평.결과 재활동기랑창환자외주혈단일핵세포중가견유Th17세포,기IL-17적형광강도위(127.6±20.5),명현고우정상인IL-17형광강도,기치위(40.6±11.1),P＜0.001.재활동기SLE환자수손적피부조직화폐조직중가견유Th17세포적침윤,이정상인피부미견유Th17세포적침윤.활동기SLE환자외주혈중Th17비례명현증가,차여SLE활동지수(SLEDAI)정정상관.상관세포인자IL-17A화IL-17F mRNA표체명현증가,혈청중적불IL-17수평분비증가,활동기SLE환자중Th17증가여혈관염적발병정정상관,경과치료후Th17수병정완감이감소.결론 Th17세포아군재활동기SLE환자체내확증,기확증여SLE활동밀절상관,가능삼여SLE 혈관염적발생.
objective To investigate the tissue localization of CD4+T cells producing IL-17,namely Th17 cells.in patients with systemic lupus erythematosus (SLE),as well as its relationship with the activity of lupus.Methods By using H&E staining.double-label immunofluorescence.immunohistochemistry and confocal microscopy.the localization of Th17 cells was carried out in peripheral blood mononuclear cells (PBMCs).affected tissue of skin and lung obtained from 4 patients with active SLE and 2 normal human controls.Flow cytometry.reverse transcription PCR.ELISA were used to detect the proportion of Th17 cells in PBMCs,the mRNA expression of interleukin-17(IL-17)A and IL-17 F,and serum level of interleukin 17,respectively,in 50 consecutive adult patients with SLE and 15 normal human controls.Results Th17 cells were detected in PBMCs of patients with active SLE.and the fuorescence intensity of IL-17 was significantly higher in patients with active SLE than in normal human controls(127.6±20.5 vs 40.6±11.1,P＜0.001).Infiltrates of Th17 cells were noted in both skin and lung tissues of patients with active SLE.but not in those of normal human controls.The proportion of Th17 cells in PBMCs was increased in patients with active SLE.and the proportion positively correlated with SLE disease activity index(SLEDAI) (r=0.725,P＜0.01).Further more.a significant increase was observed in the mRNA expression of IL-17 A and IL-17 F and serum level of IL-17 in patients with active SLE compared with normal human controls.The amount of Th17 cells was positively correlated with the development of vasculitis.and it experienced a decrease with the remission of SLE.Conclusions A proliferation of Th17 cells is noted in patients with active SLE.which seems to closely correlated with the activity of SLE and may take part in the development of vasculitis in SLE.
