中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2010年
11期
1361-1363
,共3页
姚刚%程翅%张琳%邓胜利%刘兴奎%喻田
姚剛%程翅%張琳%鄧勝利%劉興奎%喻田
요강%정시%장림%산성리%류흥규%유전
缺氧%二氮嗪%细胞低氧%氧%钙网蛋白
缺氧%二氮嗪%細胞低氧%氧%鈣網蛋白
결양%이담진%세포저양%양%개망단백
Anoxia%Diazoxide%Cell hypoxia%Oxygen%Calreticulin
目的 探讨缺氧后处理和二氮嗪后处理对大鼠心肌细胞缺氧复氧时钙网蛋白(CRT)表达的影响.方法 健康雄性SD大鼠,4～5月龄,分离心肌细胞,培养18 h后,随机分为6组(n=8):对照组(C组)、缺氧复氧组(HR组)、缺氧后处理组(HP组)和二氮嗪后处理组(DP组).C组细胞于5%CO2培养箱内继续培养2 h;HR组细胞于95%N2-5%CO2培养箱中缺氧45 min,然后于95%O2-5%CO2培养箱中复氧1 h;HP组细胞于95%N2-5%CO2培养箱中缺氧45 min,然后复氧3 min,缺氧3 min,重复3次,再于95%O2-5%CO2培养箱中复氧1 h;DP组细胞于95%N2-5%CO2培养箱中缺氧45 min,然后给予50 μmol/L二氮嗪处理10 min,再于95%O2-5%CO2培养箱中复氧1 h.测定心肌细胞caspase-3活性、CRT表达和游离钙离子浓度.结果 与C组比较,其余各组caspase-3活性升高,HP组和DP组CRT表达上调,HR组游离钙离子浓度升高(P＜0.05或0.01);与HR组比较,HP组和DP组caspase-3活性降低,CRT表达上调,游离钙离子浓度降低(P＜0.01).结论 缺氧后处理和二氮嗪后处理可上调CRT的表达,减轻细胞内钙超载,减轻大鼠心肌细胞缺氧复氧损伤.
目的 探討缺氧後處理和二氮嗪後處理對大鼠心肌細胞缺氧複氧時鈣網蛋白(CRT)錶達的影響.方法 健康雄性SD大鼠,4～5月齡,分離心肌細胞,培養18 h後,隨機分為6組(n=8):對照組(C組)、缺氧複氧組(HR組)、缺氧後處理組(HP組)和二氮嗪後處理組(DP組).C組細胞于5%CO2培養箱內繼續培養2 h;HR組細胞于95%N2-5%CO2培養箱中缺氧45 min,然後于95%O2-5%CO2培養箱中複氧1 h;HP組細胞于95%N2-5%CO2培養箱中缺氧45 min,然後複氧3 min,缺氧3 min,重複3次,再于95%O2-5%CO2培養箱中複氧1 h;DP組細胞于95%N2-5%CO2培養箱中缺氧45 min,然後給予50 μmol/L二氮嗪處理10 min,再于95%O2-5%CO2培養箱中複氧1 h.測定心肌細胞caspase-3活性、CRT錶達和遊離鈣離子濃度.結果 與C組比較,其餘各組caspase-3活性升高,HP組和DP組CRT錶達上調,HR組遊離鈣離子濃度升高(P＜0.05或0.01);與HR組比較,HP組和DP組caspase-3活性降低,CRT錶達上調,遊離鈣離子濃度降低(P＜0.01).結論 缺氧後處理和二氮嗪後處理可上調CRT的錶達,減輕細胞內鈣超載,減輕大鼠心肌細胞缺氧複氧損傷.
목적 탐토결양후처리화이담진후처리대대서심기세포결양복양시개망단백(CRT)표체적영향.방법 건강웅성SD대서,4～5월령,분리심기세포,배양18 h후,수궤분위6조(n=8):대조조(C조)、결양복양조(HR조)、결양후처리조(HP조)화이담진후처리조(DP조).C조세포우5%CO2배양상내계속배양2 h;HR조세포우95%N2-5%CO2배양상중결양45 min,연후우95%O2-5%CO2배양상중복양1 h;HP조세포우95%N2-5%CO2배양상중결양45 min,연후복양3 min,결양3 min,중복3차,재우95%O2-5%CO2배양상중복양1 h;DP조세포우95%N2-5%CO2배양상중결양45 min,연후급여50 μmol/L이담진처리10 min,재우95%O2-5%CO2배양상중복양1 h.측정심기세포caspase-3활성、CRT표체화유리개리자농도.결과 여C조비교,기여각조caspase-3활성승고,HP조화DP조CRT표체상조,HR조유리개리자농도승고(P＜0.05혹0.01);여HR조비교,HP조화DP조caspase-3활성강저,CRT표체상조,유리개리자농도강저(P＜0.01).결론 결양후처리화이담진후처리가상조CRT적표체,감경세포내개초재,감경대서심기세포결양복양손상.
Objective To investigate the effect of hypoxic postconditioning and diazoxide postconditioning on the calreticulin expression during hypoxia/reoxygenation (H/R) in rat cardiomyocytes. Methods Primary cultured male SD rat cardiomyocytes were randomly divided into 6 groups (n = 8 each): control group (group C),H/R group; hypoxic postconditioning group (group HP) and diazoxide postconditioning group (group DP). Group C were cultured continuously for 2 h. Group H/R, Hp and DP were exposed to 45 min hypoxia (95% N2-5% CO2)followed by 1 h reoxygenation. In group HP, the cells were subjected to three cycles of 3 min hypoxia at 3 min intervals at the end of 45 min hypoxia before 1 h reoxygenation. In group DP, diazoxide 50 μmol/L was giyen at the end of hypoxia. Caspase-3 activity, calreticulin expression and intracellular free calcium ion concentrations were determined. Results Compared with group C, caspase-3 activity was significantly increased in the other groups,the calreticulin expression was up-regulated in group HP and DP, and the free calcium ion concentrations were increased in group H/R (P ＜ 0.05 or 0.01). Compared with group H/R, caspase-3 activity was significantly decreased in HP and DP, the calreticulin expression was up-regulated and the free calcium ion concentrations were decreased in group HP and DP (P ＜ 0.01). Conclusion Hypoxic postconditioning and diazoxide postconditioning attenuate H/R injury in rat cardiomyocytes through up-regulating the expression of calreticulin and reducing intracellular calcium overload.
