中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2011年
4期
452-455
,共4页
倪诚%郭向阳%钱敏%周阳%吴长毅%王军%李民%贾东林%岳峰
倪誠%郭嚮暘%錢敏%週暘%吳長毅%王軍%李民%賈東林%嶽峰
예성%곽향양%전민%주양%오장의%왕군%리민%가동림%악봉
褪黑激素%异氟醚%海马%胆碱%O-乙酰转移酶
褪黑激素%異氟醚%海馬%膽堿%O-乙酰轉移酶
퇴흑격소%이불미%해마%담감%O-을선전이매
Melatonin%Isoflurane%Hippocampus%Choline O-acetyltransferase
目的 探讨褪黑素对异氟醚麻醉大鼠海马胆碱乙酰基转移酶(ChAT)的影响.方法雄性SD大鼠60只,体重390~440 g,采用随机数字表法,将大鼠随机分为5组(n=12):对照组(C组)、1%异氟醚组(Ⅰ组)、1%异氟醚+褪黑素组(IM组)、2%异氟醚组(J组)和2%异氟醚+褪黑素组(JM组).IM组和JM组腹腔注射褪黑素10 mg/kg,1次/d,连续7 d,C组、Ⅰ组和J组给予等容量生理盐水.Ⅰ组、IM组第7天吸入1%异氟醚4 h,J组、JM组第7天吸入2%异氟醚4 h.于麻醉次日行Morris水迷宫实验,测试逃避潜伏期及原平台象限探索时间;水迷宫实验结束后取血浆及脑组织,采用ELISA法测定血浆褪黑素浓度,Western blot法测定海马ChAT表达水平,采用比色法测定海马ChAT活性,采用免疫荧光法测定海马CA1区和齿状回的ChAT阳性神经元数量.结果 与C组比较,Ⅰ组血浆褪黑素浓度、ChAT表达水平和活性降低(P<0.01);J组逃避潜伏期延长,原平台象限探索时间缩短,血浆褪黑素浓度、ChAT表达水平和活性降低(P<0.05或0.01).与Ⅰ组比较,IM组逃避潜伏期缩短,原平台象限探索时间延长,褪黑素浓度升高,ChAT表达水平和活性升高(P<0.05或0.01).与J组比较,JM组逃避潜伏期缩短,褪黑素浓度升高,ChAT活性升高(P<0.05或0.01).海马CA1区和齿状回的ChAT阳性神经元数量与ChAT表达水平变化一致.结论 褪黑素可减轻异氟醚麻醉对ChAT表达水平及活性的抑制,从而改善异氟醚麻醉后大鼠的认知功能.
目的 探討褪黑素對異氟醚痳醉大鼠海馬膽堿乙酰基轉移酶(ChAT)的影響.方法雄性SD大鼠60隻,體重390~440 g,採用隨機數字錶法,將大鼠隨機分為5組(n=12):對照組(C組)、1%異氟醚組(Ⅰ組)、1%異氟醚+褪黑素組(IM組)、2%異氟醚組(J組)和2%異氟醚+褪黑素組(JM組).IM組和JM組腹腔註射褪黑素10 mg/kg,1次/d,連續7 d,C組、Ⅰ組和J組給予等容量生理鹽水.Ⅰ組、IM組第7天吸入1%異氟醚4 h,J組、JM組第7天吸入2%異氟醚4 h.于痳醉次日行Morris水迷宮實驗,測試逃避潛伏期及原平檯象限探索時間;水迷宮實驗結束後取血漿及腦組織,採用ELISA法測定血漿褪黑素濃度,Western blot法測定海馬ChAT錶達水平,採用比色法測定海馬ChAT活性,採用免疫熒光法測定海馬CA1區和齒狀迴的ChAT暘性神經元數量.結果 與C組比較,Ⅰ組血漿褪黑素濃度、ChAT錶達水平和活性降低(P<0.01);J組逃避潛伏期延長,原平檯象限探索時間縮短,血漿褪黑素濃度、ChAT錶達水平和活性降低(P<0.05或0.01).與Ⅰ組比較,IM組逃避潛伏期縮短,原平檯象限探索時間延長,褪黑素濃度升高,ChAT錶達水平和活性升高(P<0.05或0.01).與J組比較,JM組逃避潛伏期縮短,褪黑素濃度升高,ChAT活性升高(P<0.05或0.01).海馬CA1區和齒狀迴的ChAT暘性神經元數量與ChAT錶達水平變化一緻.結論 褪黑素可減輕異氟醚痳醉對ChAT錶達水平及活性的抑製,從而改善異氟醚痳醉後大鼠的認知功能.
목적 탐토퇴흑소대이불미마취대서해마담감을선기전이매(ChAT)적영향.방법웅성SD대서60지,체중390~440 g,채용수궤수자표법,장대서수궤분위5조(n=12):대조조(C조)、1%이불미조(Ⅰ조)、1%이불미+퇴흑소조(IM조)、2%이불미조(J조)화2%이불미+퇴흑소조(JM조).IM조화JM조복강주사퇴흑소10 mg/kg,1차/d,련속7 d,C조、Ⅰ조화J조급여등용량생리염수.Ⅰ조、IM조제7천흡입1%이불미4 h,J조、JM조제7천흡입2%이불미4 h.우마취차일행Morris수미궁실험,측시도피잠복기급원평태상한탐색시간;수미궁실험결속후취혈장급뇌조직,채용ELISA법측정혈장퇴흑소농도,Western blot법측정해마ChAT표체수평,채용비색법측정해마ChAT활성,채용면역형광법측정해마CA1구화치상회적ChAT양성신경원수량.결과 여C조비교,Ⅰ조혈장퇴흑소농도、ChAT표체수평화활성강저(P<0.01);J조도피잠복기연장,원평태상한탐색시간축단,혈장퇴흑소농도、ChAT표체수평화활성강저(P<0.05혹0.01).여Ⅰ조비교,IM조도피잠복기축단,원평태상한탐색시간연장,퇴흑소농도승고,ChAT표체수평화활성승고(P<0.05혹0.01).여J조비교,JM조도피잠복기축단,퇴흑소농도승고,ChAT활성승고(P<0.05혹0.01).해마CA1구화치상회적ChAT양성신경원수량여ChAT표체수평변화일치.결론 퇴흑소가감경이불미마취대ChAT표체수평급활성적억제,종이개선이불미마취후대서적인지공능.
Objective To investigate the effects of melatonin on choline acetyltransferase (ChAT) in rat hippocampus after isoflurane anesthesia. Methods Sixty male SD rats weighing 390 - 440 g were randomized into 5 groups (n = 12 each): control group (group C), 1% isoflurane group (group Ⅰ), 1% isoflurane + melatonin group (group IM) , 2% isoflurane group (group J) and 2% isoflurane + melatonin group (group JM) . In IM and JM groups, melatonin 10 mg/kg was administered intraperitoneally once a day for 7 consecutive days, while equal volume of normal saline was given intraperitoneally instead of melatonin in C, I and J groups. Groups Ⅰ and IM inhaled 1% isoflurane and groups J and JM 2% isoflurane for 4 h on 7th day. All the rats underwent Morris water maze test on the day after anesthesia for assessment of learning and memory ability (escape latency and probe time) . The training test was performed 4 times a day for S days. Six rats randomly selected from each group were sacrificed the end of the test. The blood samples were collected for detection of plasma melatonin level by ELISA.The brain tissues were removed for determination of the expression and activity of ChAT in hippocampus by Western blot or colorimetric assay. The left rats were selected and sacrificed for determination of the number of ChAT positive neurons in hippocampal CA1 region and entate gyrus by immunofluorescence. Results The plasma melatonin level and expression and activity of ChAT were significantly lower in group I than in group C ( P < 0.01) . The escape latency was significantly longer, the probe time was significantly shorter, and the plasma melatonin level and expression and activity of ChAT were significantly lower in group J than in group C ( P < 0.05 or 0.01) . The escape latency was significantly shorter, the probe time was significantly longer, and the plasma melatonin level and expression and activity of ChAT were significantly higher in group IM than in group Ⅰ ( P < 0.05 or 0.01). The escape latency was significantly shorter and the plasma melatonin level and ChAT activity were significantly higher in group JM than in group J ( P < 0.05 or 0.01) . The results of immunofluorescent staining showed that the number of ChAT positive neurons in hippocampal CA1 region and dentate gyrus wag consistent with the changes in the measured ChAT expression. Conclusion Melatonin can reduce isoflurane-mediated inhibition of ChAT expression and activity and thus improve spatial memory impaired by isoflurane anesthesia in rats.