CAJ | 학술논문

目的对纳米银溶胶的体外细胞毒性进行评价,初步探讨纳米银对细胞的毒性作用机制.方法利用化学还原法制备纳米银溶胶,通过紫外-可见分光光度计和透射电镜对其物理特性进行检测；以小鼠纤维肉瘤细胞( L929)为研究对象,通过细胞形态观察、LIVE/DEAD染色分析和MTT检测来评价纳米银对细胞的毒性作用；在电子显微镜下观察纳米银在细胞中的分布,探讨纳米银对细胞的毒性作用机制.结果当纳米银质量浓度大于10 mg/L时会对L929细胞产生明显的细胞毒性,细胞形态发生明显变化,细胞增殖受到抑制.纳米银可以通过胞吞作用进入细胞,与细胞内的多种细胞器相互作用而造成细胞损伤.结论纳米银会对哺乳动物细胞的形态和存活产生影响且具有浓度依赖性,因此在纳米银的应用过程中要考虑其毒副作用.
목적 대납미은용효적체외세포독성진행평개,초보탐토납미은대세포적독성작용궤제.방법이용화학환원법제비납미은용효,통과자외-가견분광광도계화투사전경대기물리특성진행검측；이소서섬유육류세포( L929)위연구대상,통과세포형태관찰、LIVE/DEAD염색분석화MTT검측래평개납미은대세포적독성작용；재전자현미경하관찰납미은재세포중적분포,탐토납미은대세포적독성작용궤제.결과 당납미은질량농도대우10 mg/L시회대L929세포산생명현적세포독성,세포형태발생명현변화,세포증식수도억제.납미은가이통과포탄작용진입세포,여세포내적다충세포기상호작용이조성세포손상.결론 납미은회대포유동물세포적형태화존활산생영향차구유농도의뢰성,인차재납미은적응용과정중요고필기독부작용.
Objective To evaluate the in vitro cytotoxicity of silver nanoparticles and investigate the toxicity mechanism of Nano Silver on cells.Methods Silver colloid was prepared by a modified chemical reduction method,and its physical properties were tested by ultraviolet-visible (UV-Vis) spectrometry and transmission electron microscopy (TEM).The in vitro cytotoxicity of the silver nanoparticles was investigated using mouse fibrosarcoma cells (L929).The toxicity was evaluated by the changes of cell morphology,LIVE/DEAD staining and 3-(4,5-dimethylthiazol-2-yl)-2,5-dipheny(I)-tetrazolium bromide (MTT) assay.In order to study the biodistribution of Nano Silver in the cells,TEM analyses of the L929 cells treated with 20.0 μg/mL of nanoparticles were performed.Results Silver nanoparticle doses of 10 μg/mL could cause obvious toxicity to L929 cells,including morphology changes and the suppression of proliferation.The nanoparticles entered into the cells through endocytosis,and interacted with organelles to induce cellular damage.Conclusions The survival and morphology of mammal cells were significantly affected by silver nanoparticles in a dose dependent manner,and the cytotoxicity should be taken into account in the application of silver nanoparticles.