中华实验眼科杂志
中華實驗眼科雜誌
중화실험안과잡지
CHINESE JOURNAL OF EXPERIMENTAL OPHTHALMOLOGY
2011年
2期
135-138
,共4页
史才财%张兴儒%周欢明%李青松%项敏泓%唐剑敏%张隆
史纔財%張興儒%週歡明%李青鬆%項敏泓%唐劍敏%張隆
사재재%장흥유%주환명%리청송%항민홍%당검민%장륭
硫酸镍%晶状体%白内障
硫痠鎳%晶狀體%白內障
류산얼%정상체%백내장
Nickel sulphate%Lens%Cataract
背景 流行病学调查表明,镍矿区人群中白内障的发病率高于其他人群,硫酸镍对人晶状体上皮细胞(LECs)的代谢和增生有抑制作用,但目前镍对晶状体损伤机制的研究较少.目的 探讨硫酸镍对SD大鼠晶状体的损伤情况.方法出生7～14d的SPF级SD大鼠45只随机分为空白对照组、皮下注射组、腹腔注射组.腹腔注射组、皮下注射组分别腹腔注射或皮下注射2g/L的硫酸镍溶液10mg/kg,连续给药45d;空白对照组不用药.给药后每2周裂隙灯下观察SD大鼠晶状体的变化,给药后45d参考LOCSⅡ、LOCSⅢ的评价标准对晶状体的前囊、核区、皮质和后囊进行混浊指数评分,评价SD大鼠晶状体的混浊程度.45d后处死大鼠,取下完整晶状体行组织学观察.结果 与空白对照组比较,皮下注射组大鼠晶状体前囊区混浊指数评分较高,差异有统计学意义(t＝14.311,P<0.05),腹腔注射组大鼠晶状体前囊区混浊指数评分虽高于空白对照组,但差异无统计学意义(t＝4.355,P>0.05).皮下注射组和腹腔注射组大鼠晶状体后囊混浊指数评分高于空白对照组,差异均有统计学意义(t＝9.316,P＝0.004;t＝7.464,P＝0.009).皮下注射组和腹腔注射组大鼠晶状体前囊+后囊晶状体混浊指数评分高于空白对照组,差异均有统计学意义(t＝23.387,P＝0.000;t＝10.533,P＝0.002).各组大鼠晶状体皮质区和核区混浊指数评分比较差异均无统计学意义(P>0.05).皮下注射组、腹腔注射组大鼠晶状体混浊指数总评分高于空白对照组,差异均有统计学意义(t＝12.358,P＝0.001;t＝10.188,P＝0.003);皮下注射组晶状体混浊的总评分高于腹腔注射组,但差异无统计学意义(t＝0.527,P>0.05).皮下注射组和腹腔注射组大鼠晶状体胶原纤维变性均比空白对照组严重,皮下注射组大鼠的晶状体胶原纤维变性比腹腔注射组明显.结论 硫酸镍皮下注射和腹腔注射均能对SD大鼠晶状体造成一定的损伤,晶状体损伤的部位主要集中在晶状体前囊和后囊下.
揹景 流行病學調查錶明,鎳礦區人群中白內障的髮病率高于其他人群,硫痠鎳對人晶狀體上皮細胞(LECs)的代謝和增生有抑製作用,但目前鎳對晶狀體損傷機製的研究較少.目的 探討硫痠鎳對SD大鼠晶狀體的損傷情況.方法齣生7～14d的SPF級SD大鼠45隻隨機分為空白對照組、皮下註射組、腹腔註射組.腹腔註射組、皮下註射組分彆腹腔註射或皮下註射2g/L的硫痠鎳溶液10mg/kg,連續給藥45d;空白對照組不用藥.給藥後每2週裂隙燈下觀察SD大鼠晶狀體的變化,給藥後45d參攷LOCSⅡ、LOCSⅢ的評價標準對晶狀體的前囊、覈區、皮質和後囊進行混濁指數評分,評價SD大鼠晶狀體的混濁程度.45d後處死大鼠,取下完整晶狀體行組織學觀察.結果 與空白對照組比較,皮下註射組大鼠晶狀體前囊區混濁指數評分較高,差異有統計學意義(t＝14.311,P<0.05),腹腔註射組大鼠晶狀體前囊區混濁指數評分雖高于空白對照組,但差異無統計學意義(t＝4.355,P>0.05).皮下註射組和腹腔註射組大鼠晶狀體後囊混濁指數評分高于空白對照組,差異均有統計學意義(t＝9.316,P＝0.004;t＝7.464,P＝0.009).皮下註射組和腹腔註射組大鼠晶狀體前囊+後囊晶狀體混濁指數評分高于空白對照組,差異均有統計學意義(t＝23.387,P＝0.000;t＝10.533,P＝0.002).各組大鼠晶狀體皮質區和覈區混濁指數評分比較差異均無統計學意義(P>0.05).皮下註射組、腹腔註射組大鼠晶狀體混濁指數總評分高于空白對照組,差異均有統計學意義(t＝12.358,P＝0.001;t＝10.188,P＝0.003);皮下註射組晶狀體混濁的總評分高于腹腔註射組,但差異無統計學意義(t＝0.527,P>0.05).皮下註射組和腹腔註射組大鼠晶狀體膠原纖維變性均比空白對照組嚴重,皮下註射組大鼠的晶狀體膠原纖維變性比腹腔註射組明顯.結論 硫痠鎳皮下註射和腹腔註射均能對SD大鼠晶狀體造成一定的損傷,晶狀體損傷的部位主要集中在晶狀體前囊和後囊下.
배경 류행병학조사표명,얼광구인군중백내장적발병솔고우기타인군,류산얼대인정상체상피세포(LECs)적대사화증생유억제작용,단목전얼대정상체손상궤제적연구교소.목적 탐토류산얼대SD대서정상체적손상정황.방법출생7～14d적SPF급SD대서45지수궤분위공백대조조、피하주사조、복강주사조.복강주사조、피하주사조분별복강주사혹피하주사2g/L적류산얼용액10mg/kg,련속급약45d;공백대조조불용약.급약후매2주렬극등하관찰SD대서정상체적변화,급약후45d삼고LOCSⅡ、LOCSⅢ적평개표준대정상체적전낭、핵구、피질화후낭진행혼탁지수평분,평개SD대서정상체적혼탁정도.45d후처사대서,취하완정정상체행조직학관찰.결과 여공백대조조비교,피하주사조대서정상체전낭구혼탁지수평분교고,차이유통계학의의(t＝14.311,P<0.05),복강주사조대서정상체전낭구혼탁지수평분수고우공백대조조,단차이무통계학의의(t＝4.355,P>0.05).피하주사조화복강주사조대서정상체후낭혼탁지수평분고우공백대조조,차이균유통계학의의(t＝9.316,P＝0.004;t＝7.464,P＝0.009).피하주사조화복강주사조대서정상체전낭+후낭정상체혼탁지수평분고우공백대조조,차이균유통계학의의(t＝23.387,P＝0.000;t＝10.533,P＝0.002).각조대서정상체피질구화핵구혼탁지수평분비교차이균무통계학의의(P>0.05).피하주사조、복강주사조대서정상체혼탁지수총평분고우공백대조조,차이균유통계학의의(t＝12.358,P＝0.001;t＝10.188,P＝0.003);피하주사조정상체혼탁적총평분고우복강주사조,단차이무통계학의의(t＝0.527,P>0.05).피하주사조화복강주사조대서정상체효원섬유변성균비공백대조조엄중,피하주사조대서적정상체효원섬유변성비복강주사조명현.결론 류산얼피하주사화복강주사균능대SD대서정상체조성일정적손상,정상체손상적부위주요집중재정상체전낭화후낭하.
Background Researches showed that the incidence rate of cataract is high in the nickel mining area. Nickel sulphate can apparently inhibit the metabolism and proliferation of human lens epithelium cells. But the study on the injury mechanism of nickel on lens is still seldom. Objective Present study was to investigate the effect of nickel sulphate on the lens of SD rats. Methods Forty-five SPF SD rats aged from 7 to 14 days were grouped randomly into subcutaneous injection group, intraperitoneal injection group and blank group. Nickel sulphate of 2 g/L ( 10 mg/kg) was subcutaneously or intraperitonealy injected for 45 days. The opacity of rat lens was examined under the slit lamp at two-week interval and scored based on the criteria of LOCS II and LOCS III. The rats were sacrificed in 45 days after experiment and the lens were obtained for the pathological examination. Result The mean score of the anterior subcapsule opacity of rat lens was obviously higher in subcutaneous injection group compared with blank control group with a significant difference between them (t= 14. 311, P < 0. 05 ) , but no significant difference in the anterior subcapsule opacity between intraperitoneal injection group and blank control group (t = 4. 355 , P>0. 05 ). The score of posterior subcapsule opacity of lens were evidently higher in both subcutaneous injection group and intraperitoneal injection group than the blank control group (t = 9. 316,P = 0. 004;t = 7. 464, P = 0. 009) ,so was the mean score of the anterior +posterior subcapsule opacities(t = 23. 387,P=0. 000;t= 10. 533,P = 0. 002) and the total score of rat lens opacity ( t = 12. 358 , P = 0. 001; t = 10. 188 , P = 0. 003 ) . No significant differences were found in cortex opacity score and nuclear opacity score among three groups ( P > 0.05 ). Histopathology examination revealed that the degeneration of lens collagen protein was more serious in subcutaneous injection group and intraperitoneal injection group than the blank control group,and the injury degree of lens collagen protein was more dominant in subcutaneous injection group. Conclusion System administration of nickel sulphate induced the injury of anterior and posterior subcapsule of lens in SD rat.
