CAJ | 학술논문

目的制作阿霉素诱导大白鼠胎仔出现先天性十二指肠闭锁的模型.方法 Wistar孕鼠10只,于妊娠第6至第9天腹腔注射阿霉素1.75 mg/kg,妊娠20天剖宫取胎仔,做解剖、电镜及光镜观察.结果用药组获胎仔32只,十二指肠闭锁26例(81.3%),其中腔内闭锁15.4%,闭锁近远端由纤维或胰腺相连者占46.2%,近远端游离者为38.5%,十二指肠闭锁畸形均伴有胰腺发育异常,9例胰颈、胰体及胰尾缺如,17例胰体和胰尾缺如,另外,镜下见部分胰腺腺泡呈发育不良改变.结论阿霉素诱导大鼠胎仔出现先天性十二指肠畸形和胰腺畸形是一种可靠的模型制作方法 .
목적 제작아매소유도대백서태자출현선천성십이지장폐쇄적모형.방법 Wistar잉서10지,우임신제6지제9천복강주사아매소1.75 mg/kg,임신20천부궁취태자,주해부、전경급광경관찰.결과 용약조획태자32지,십이지장폐쇄26례(81.3%),기중강내폐쇄15.4%,폐쇄근원단유섬유혹이선상련자점46.2%,근원단유리자위38.5%,십이지장폐쇄기형균반유이선발육이상,9례이경、이체급이미결여,17례이체화이미결여,령외,경하견부분이선선포정발육불량개변.결론 아매소유도대서태자출현선천성십이지장기형화이선기형시일충가고적모형제작방법 .
Objective To describe a reproducible fetal model of duodenal atresia.Methods Timemated pregnant rats were given 1.75 mg/kg of Adriamycin intraperitoneally on days 6 through 9 of gestation,and the litters were recovered on day 20.The fetuses were dissected microscopically and studied histologically.The findings were compared with those of age-matched saline embryos.Results All saline embryos were normal,whereas 81.7%(26/32)of Adriamycin embryos had duodenal atresia.The intraluminal atresia was found in 15.4%(4/26)of duodenal atresia embryos,gapped atresia with pancreatic tissue filled in between in 46.2%(12/26)and gapped atresia in 38.5%(10/26).A double duodenal atresia was found in one fetus.The malformation was anatomically identical to that of human neonates.Interestingly,all duodenal atresia embryos were associated with pancreatic agenesis,in 34.6% with the pancreatic neck,body and tail absence and in 65.4% with the body and tail absence.Conclusion This easily reproducible experimental model permits new research into both its embryogenesis and the biology of the duodenal atresia fetus.