CAJ | 학술논문

目的探讨亚甲蓝对兔肝缺血再灌注损伤的影响.方法健康成年新西兰大白兔24只,雌雄不拘,体重2.0～2.3 kg,随机分为3组(n=8):假手术组(S组)、肝缺血再灌注组(I/R组)和亚甲蓝组(MB组).I/R组及MB组采用夹闭肝左外叶、中叶、右中叶及方形叶肝动脉分支40min再灌注60 min的方法制备肝缺血再灌注模型,S组仅游离相应血管.MB组于再灌注前20 min经耳缘静脉注射亚甲蓝5 mg/kg(用生理盐水稀释至5 ml),S组及I/R组给予等容量生理盐水.于缺血前即刻(T1)、缺血20 min(T2)、加min(T3)、再灌注1 min(T4)、再灌注5 min(T5)、30 min(T6)、60 min(T7)时记录MAP和HR.于T1,5-7时取股动脉血样1 ml,测定血清TNF-α及IL-6的浓度.分别于T1,6,7时取股动脉血样1.5 ml,测定血浆ALT及AST的活性.于T7时测定肝左叶组织SOD活性及MDA含量,光镜下观察肝组织病理学结果.结果与S组比较,I/R组T4-7,时MAP降低,T7时HR降低,肝组织SOD活性降低,MDA含量升高,I/R组及MB组T3-5时血清TNF-α和IL-6浓度升高,T6,7时血浆ALT和AST活性升高(P<0.05或0.01);与I/R组比较,MB组T4-7时MAP升高,肝组织SOD活性升高,MDA含量降低,T3-5时血清TNF-α和IL-6浓度降低,T6,7时血浆ALT和AST活性降低(P<0.05或0.01).MB组肝组织损伤较I/R组减轻.结论亚甲蓝可维持血液动力学稳定,减轻兔肝缺血再灌注损伤.
목적 탐토아갑람대토간결혈재관주손상적영향.방법 건강성년신서란대백토24지,자웅불구,체중2.0～2.3 kg,수궤분위3조(n=8):가수술조(S조)、간결혈재관주조(I/R조)화아갑람조(MB조).I/R조급MB조채용협폐간좌외협、중협、우중협급방형협간동맥분지40min재관주60 min적방법제비간결혈재관주모형,S조부유리상응혈관.MB조우재관주전20 min경이연정맥주사아갑람5 mg/kg(용생리염수희석지5 ml),S조급I/R조급여등용량생리염수.우결혈전즉각(T1)、결혈20 min(T2)、가min(T3)、재관주1 min(T4)、재관주5 min(T5)、30 min(T6)、60 min(T7)시기록MAP화HR.우T1,5-7시취고동맥혈양1 ml,측정혈청TNF-α급IL-6적농도.분별우T1,6,7시취고동맥혈양1.5 ml,측정혈장ALT급AST적활성.우T7시측정간좌협조직SOD활성급MDA함량,광경하관찰간조직병이학결과.결과 여S조비교,I/R조T4-7,시MAP강저,T7시HR강저,간조직SOD활성강저,MDA함량승고,I/R조급MB조T3-5시혈청TNF-α화IL-6농도승고,T6,7시혈장ALT화AST활성승고(P<0.05혹0.01);여I/R조비교,MB조T4-7시MAP승고,간조직SOD활성승고,MDA함량강저,T3-5시혈청TNF-α화IL-6농도강저,T6,7시혈장ALT화AST활성강저(P<0.05혹0.01).MB조간조직손상교I/R조감경.결론 아갑람가유지혈액동역학은정,감경토간결혈재관주손상.
Objective To investigate the effect of methylene blue(MB)on liver ischemia-reperfusion (I/R) injury in rabbits.Methods Twenty-four healthy New Zealand white rabbits of both sexes welshins 2.0-2.3 kg were randomly divided into 3 groups (n=8 each):group Ⅰ sham operation(group s);group Ⅱ I/R and group Ⅲ methylene blue (group MB).The animals were anesthetized with intravenous 2% pentobarbital 30 mg/kg.Liver I/R was produced by occlusion of hepatic blood flow for 40 min followed by 60 min repeffusion.In group MB methylene blue 5 mg/kg was injected iv at 20 min before liver ischemia.Femoral artery was carmulated for MAP monitoring and blood sampling.MAP and HR were recorded immediately before(T1,baseline)and at 20 and 40 min of ischemia (T2,3) and 1,5,30,60 min(T4-7)of repedusion.Blood samples were collected at T1,T5,T6 and T7 for measurement of seruln TNF-α and IL-6 concentrations.Plasma AST and ALT activities were measured at T1,T6 and T7.Liver specimens were obtained at the end of experiment for determination of SOD activity and MDA content.Results In group I/R MAP was significantly decreased at T4-7 during reperfusion and HR at T7 as compared with the baseline at T1;while in group MB no significant change in MAP and HR Was observed during ischemia and reperfusion as compared with the baseline.The gerum TNF-α and IL-6 concentrations and the plasma ALT and AST activities were significantly increased during reperfusion as compared with the baseline immediately before ischemia in group I/R and MB and were significantly lower in group MB than in group. I/R. The SOD activity was significantly higher while MDA content was significantly lower in group MB than in group I/R. Microscopic examination showed that liver damage was less severe in group MB than in group I/R. Conclusion The administration of MB can maintain hemodynamic stability and attenuate liver I/R injury in rabbits.