CAJ | 학술논문

目的观察原位肾低温灌注动物模型的制作以及低温灌注对术中肾细胞的保护作用.方法自制肾动脉阻断灌注装置,建立猪肾在体循环低温灌注模型,随机分为3组:假手术对照组、低温灌注组和动脉阻断组(n=8).常规病理学检查、免疫组织化学及透射电镜观察肾组织、细胞形态及超微结构变化.结果阻断后0.5 h,动脉阻断组肾出现细胞核及线粒体肿胀,胞质水变性.与假手术对照组和低温灌注组比较,细胞凋亡指数(AI)(1.3±0.4)%及Caspase-3蛋白(IOD:2.42±0.62)明显增高(P＜0.05);低温灌注组肾组织仅出现细胞质水变性,细胞核及线粒体未发生明显变化.阻断1 h后,动脉阻断组肾在光镜下可见部分细胞凋亡、坏死,电镜下可见细胞核固缩、核膜轻度溶解,线粒体高度肿胀;而低温灌注组光镜下未见细胞坏死,仅见细胞核及线粒体肿胀、空泡变性.结论该模型简单易行,低温灌注能有效保护肾组织.
목적 관찰원위신저온관주동물모형적제작이급저온관주대술중신세포적보호작용.방법 자제신동맥조단관주장치,건립저신재체순배저온관주모형,수궤분위3조:가수술대조조、저온관주조화동맥조단조(n=8).상규병이학검사、면역조직화학급투사전경관찰신조직、세포형태급초미결구변화.결과 조단후0.5 h,동맥조단조신출현세포핵급선립체종창,포질수변성.여가수술대조조화저온관주조비교,세포조망지수(AI)(1.3±0.4)%급Caspase-3단백(IOD:2.42±0.62)명현증고(P＜0.05);저온관주조신조직부출현세포질수변성,세포핵급선립체미발생명현변화.조단1 h후,동맥조단조신재광경하가견부분세포조망、배사,전경하가견세포핵고축、핵막경도용해,선립체고도종창;이저온관주조광경하미견세포배사,부견세포핵급선립체종창、공포변성.결론 해모형간단역행,저온관주능유효보호신조직.
Objective To establish the low temperature perfusion model of pig kidneys in vivo and investigate the protective effect of low temperature perfusion on pig kidney cells. Methods In vivo pig kidneys were randomly divided into 3 groups ( n = 8 ): sham-operation group, low temperature perfusion group and arterial occlusion group. The samples were examined histopathologically. The expression of Caspase-3protein in renal cells was detected by using immunohistochemistry. The ultrastructure of renal tissue was observed under the electron microscopy. Results At the 30th h after occlusion, there was swelling of nuclei and mitochondria and hydropic degeneration of cytoplasma in arterial occlusion group. As compared with sham-operation group and low temperature perfusion group, apoptosis index (AI) [( 1.3 ± 0. 4) %]and the expression of Caspase-3 [( IOD: 2. 42 ± 0. 62)] were significantly increased as compared with arterial occlusion group (P ＜ 0. 05). At the 1st h after occlusion, apoptosis and necrosis of some cells were seen under the ligh microscopy, pyknosis of nuclei, mild lysis of nuclear membrane and severe swelling of mitochondria were observed under the electron microscopy in arterial occlusion group. In low temperature perfusion group, there was no necrosis, and swellsing and vacuolar degeneration of nuclei and mitochondria were observed under the light microscopy. Conclusion This model is easy and convenient, and the low temperature perfusion could protect function of the kidney efficiently.