CAJ | 학술논문

目的应用蛋白质组学寻找溃疡性结肠炎(UC)血清差异蛋白,初步探索UC可能的生物标志物.方法收集UC患者30例和健康对照者30名的血清标本,双向凝胶电泳(2-DE)分离等量混合血清的蛋白质,运用图像分析软件进行比较和分析,识别差异表达蛋白质.应用基质辅助激光解吸/电离飞行时间质谱(MALDI-TOF-MS)鉴定部分差异蛋白质点.结果 UC组和对照组之间年龄、体重指数、吸烟情况和饮酒量的差异均无统计学意义(P值均＞0.05).初步筛选出UC患者与健康对照者存在明显差异的39个蛋白点,选择其中9个点,经质谱分析发现触珠蛋白、热休克转录因子2、受体酪氨酸激酶、醛脱氢酶、载脂蛋白C-Ⅲ、中心粒旁物质1在UC患者中表达水平升高,角蛋白1、细丝蛋白A结合蛋白1、肌球蛋白3在UC患者中表达水平降低.结论采用蛋白质组学2-DE和质谱技术,筛选并鉴定出与UC相关的9个血清蛋白质,为提供新的UC生物学行为研究分子标志物奠定基础.
목적 응용단백질조학심조궤양성결장염(UC)혈청차이단백,초보탐색UC가능적생물표지물.방법 수집UC환자30례화건강대조자30명적혈청표본,쌍향응효전영(2-DE)분리등량혼합혈청적단백질,운용도상분석연건진행비교화분석,식별차이표체단백질.응용기질보조격광해흡/전리비행시간질보(MALDI-TOF-MS)감정부분차이단백질점.결과 UC조화대조조지간년령、체중지수、흡연정황화음주량적차이균무통계학의의(P치균＞0.05).초보사선출UC환자여건강대조자존재명현차이적39개단백점,선택기중9개점,경질보분석발현촉주단백、열휴극전록인자2、수체락안산격매、철탈경매、재지단백C-Ⅲ、중심립방물질1재UC환자중표체수평승고,각단백1、세사단백A결합단백1、기구단백3재UC환자중표체수평강저.결론 채용단백질조학2-DE화질보기술,사선병감정출여UC상관적9개혈청단백질,위제공신적UC생물학행위연구분자표지물전정기출.
Objective To search for the differences of serum proteins expression in ulcerative colitis (UC) by proteomics method and to preliminary explore the potential biological markers of ulcerative colitis. Method The serum of 30 ulcerative colitis patients and 30 healthy individuals were collected. The equal amounts of proteins in pooled serum were separated by two-dimensional gel electrophoresis (2-DE) and then compared and analyzed by image analysis software to recognize the differences of protein expression. Some spots of proteins with different expression were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS).Result There was no statistic significant in age, weight index, smoking and alcohol taking between UC group and control (P ＞ 0.05 respectively). Thirty-nine proteins with significant different expression between UC patients and healthy individuals were preliminary screened out. Nine of those spots were selected, after analyzed by MALDI-TOF-MS, it was found that the expression of haptoglobin, heat shock factor protein 2, receptor tyrosine kinase, aldehyde reductase,apolipoproteinC-Ⅲ, pericentriol material 1 increased in ulcerative colitis patients, keratinl, filamin A interacting protein 1 and tropomyosin 3 decreased. Conclusions With proteomics 2-DE and spectrometry methods, nine UC associated serum proteins were screened out and identified, which provide new molecular markers for the research of UC biological behavior.