中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2009年
10期
872-874
,共3页
张欣%闫惠平%黄春%檀玉芬%马冬梅%张海萍%刘妍%王曙照
張訢%閆惠平%黃春%檀玉芬%馬鼕梅%張海萍%劉妍%王曙照
장흔%염혜평%황춘%단옥분%마동매%장해평%류연%왕서조
手足口病%肠道病毒感染%逆转录聚合酶链反应%评价研究
手足口病%腸道病毒感染%逆轉錄聚閤酶鏈反應%評價研究
수족구병%장도병독감염%역전록취합매련반응%평개연구
Hand%food and mouth disease%Enterovirus infections%Reverse transcriptase polymerase chain reaction%Evaluation study
目的 分析研究70例手足口病患者的临床一般情况及其致病原.方法 采集70例手足口病患者(其中5岁以下儿童患者60例)的咽拭子标本,提取病毒RNA.用逆转录聚合酶链反应(RT-PCR)一步法检测肠道病毒(EV)的5'-UTR区基因、肠道病毒71型(EV71)的Vp3～Vp1区基因、柯萨奇病毒A16(Cox-A16)的Vp3～Vp1区基因.结果 在70例手足口病患者中,咽拭子标本肠道病毒核酸阳性率为42.8%(30/70).在30例肠道病毒核酸阳性病原中,EV71占66.7%(20/30).5岁以下儿童有EV71肠道病毒、或Cox-A16病毒、或非EV71及非Cox-A16的其他肠道病毒感染病例.而5岁以上的儿童和成人患者则只有EV71肠道病毒感染病例.39例4 d内采集的咽拭子标本病原核酸阳性检测率平均为66.7%(26/39),31例5 d以后采集的标本病原核酸阳性检测率是12.9%(4/31),差异有统计学意义(x~2=20.4,P<0.01).结论 手足口病以0～5岁婴幼儿患病为主,但成人也可发病.用RT-PCR检测病原核酸阳性的手足口病患者以EV71为主,且以发病后4 d内阳性检测率高.
目的 分析研究70例手足口病患者的臨床一般情況及其緻病原.方法 採集70例手足口病患者(其中5歲以下兒童患者60例)的嚥拭子標本,提取病毒RNA.用逆轉錄聚閤酶鏈反應(RT-PCR)一步法檢測腸道病毒(EV)的5'-UTR區基因、腸道病毒71型(EV71)的Vp3～Vp1區基因、柯薩奇病毒A16(Cox-A16)的Vp3～Vp1區基因.結果 在70例手足口病患者中,嚥拭子標本腸道病毒覈痠暘性率為42.8%(30/70).在30例腸道病毒覈痠暘性病原中,EV71佔66.7%(20/30).5歲以下兒童有EV71腸道病毒、或Cox-A16病毒、或非EV71及非Cox-A16的其他腸道病毒感染病例.而5歲以上的兒童和成人患者則隻有EV71腸道病毒感染病例.39例4 d內採集的嚥拭子標本病原覈痠暘性檢測率平均為66.7%(26/39),31例5 d以後採集的標本病原覈痠暘性檢測率是12.9%(4/31),差異有統計學意義(x~2=20.4,P<0.01).結論 手足口病以0～5歲嬰幼兒患病為主,但成人也可髮病.用RT-PCR檢測病原覈痠暘性的手足口病患者以EV71為主,且以髮病後4 d內暘性檢測率高.
목적 분석연구70례수족구병환자적림상일반정황급기치병원.방법 채집70례수족구병환자(기중5세이하인동환자60례)적인식자표본,제취병독RNA.용역전록취합매련반응(RT-PCR)일보법검측장도병독(EV)적5'-UTR구기인、장도병독71형(EV71)적Vp3～Vp1구기인、가살기병독A16(Cox-A16)적Vp3～Vp1구기인.결과 재70례수족구병환자중,인식자표본장도병독핵산양성솔위42.8%(30/70).재30례장도병독핵산양성병원중,EV71점66.7%(20/30).5세이하인동유EV71장도병독、혹Cox-A16병독、혹비EV71급비Cox-A16적기타장도병독감염병례.이5세이상적인동화성인환자칙지유EV71장도병독감염병례.39례4 d내채집적인식자표본병원핵산양성검측솔평균위66.7%(26/39),31례5 d이후채집적표본병원핵산양성검측솔시12.9%(4/31),차이유통계학의의(x~2=20.4,P<0.01).결론 수족구병이0～5세영유인환병위주,단성인야가발병.용RT-PCR검측병원핵산양성적수족구병환자이EV71위주,차이발병후4 d내양성검측솔고.
Objective To analyze the etiology and clinical manifestations of 70 patients with hand-foot-mouth disease (HFMD). Methods The viral RNA from the pharynx swab samples were extracted and collected from 70 HFMD patients (of which,60 cases were under 5 years old) to detect the 5'-UTR gene of EV,the Vp3-Vp1 genes of EV71 and Cox-A16 by utilizing the technique of RT-PCR. Results Of 70 HFMD patients,30 cases (42.8%, 30/70) were positive in enterovirus nucleic acid, including EV71 and Cox-A16,pharynx swab sample testing. While, of the enteroviral pathogen RNA-positive 30 cases, EV71 accounted for 66. 7% (20/30). The proportion of etiological positive of 39 samples collected within 4 days after onset was 66.7% (26/39), but the probability of 31 samples collected after more than 5 days after onset was 12.9%(4/31),the difference was significantly(x~2=20.4,P<0. 01). Conclusion HFMD patients were mainly infants and young children between 0-5 years old, but adults could also be attacked. The enterovirus detected was mainly EV71,and the pharynx swab samples should be collected within 4 days after onset to increase the related viral nucleic acid positive detection probability.
