CAJ | 학술논문

目的：观察大鼠肺纤维化过程中肺内一氧化氮代谢的动态变化及其与肺纤维化形成的关系。方法：气管内一次性滴注平阳霉素（5 mL/kg），观察注后7、14、21、30 d和70 d组大鼠肺组织羟脯氨酸含量，出、入肺血NO-2/NO-3含量以及14 d组肺泡巨噬细胞培养上清液中NO-2/NO-3含量和肺间质诱导型一氧化氮合酶（iNOS）免疫组化阳性细胞数量的变化。结果： 7 d组大鼠肺组织羟脯氨酸含量与对照组比无明显差异，14 d组高于对照组（P＜0.05）, 21 d组、30 d组和70 d组更为明显（均P＜0.01）。7 d组、14 d组出肺血NO-2/NO-3含量明显高于对照组（均P＜0.01），入肺血NO-2/NO-3含量明显低于对照组（均P＜0.01），21 d组出肺血NO-2/NO-3含量的变化无明显差异（P＞0.05）, 入肺血仍较低（P＜0.01），30 d组和70 d组出、入肺血NO-2/NO-3含量与对照组无明显差异（P＞0.05）。14 d组大鼠肺泡巨噬细胞培养上清液中NO-2/NO-3含量明显高于对照组（P＜0.01）。14 d组大鼠肺间质iNOS免疫组化阳性细胞增多。结论：大鼠肺纤维化形成过程中，先有肺内NO生成增多，后出现肺纤维化；在肺纤维化形成后，肺内NO趋向恢复。肺内NO增多与肺泡巨噬细胞释放NO能力增加、肺内iNOS的增多有关。肺内NO的大量生成可能是促使肺纤维化形成的因素之一。
목적：관찰대서폐섬유화과정중폐내일양화담대사적동태변화급기여폐섬유화형성적관계。방법：기관내일차성적주평양매소（5 mL/kg），관찰주후7、14、21、30 d화70 d조대서폐조직간포안산함량，출、입폐혈NO-2/NO-3함량이급14 d조폐포거서세포배양상청액중NO-2/NO-3함량화폐간질유도형일양화담합매（iNOS）면역조화양성세포수량적변화。결과： 7 d조대서폐조직간포안산함량여대조조비무명현차이，14 d조고우대조조（P＜0.05）, 21 d조、30 d조화70 d조경위명현（균P＜0.01）。7 d조、14 d조출폐혈NO-2/NO-3함량명현고우대조조（균P＜0.01），입폐혈NO-2/NO-3함량명현저우대조조（균P＜0.01），21 d조출폐혈NO-2/NO-3함량적변화무명현차이（P＞0.05）, 입폐혈잉교저（P＜0.01），30 d조화70 d조출、입폐혈NO-2/NO-3함량여대조조무명현차이（P＞0.05）。14 d조대서폐포거서세포배양상청액중NO-2/NO-3함량명현고우대조조（P＜0.01）。14 d조대서폐간질iNOS면역조화양성세포증다。결론：대서폐섬유화형성과정중，선유폐내NO생성증다，후출현폐섬유화；재폐섬유화형성후，폐내NO추향회복。폐내NO증다여폐포거서세포석방NO능력증가、폐내iNOS적증다유관。폐내NO적대량생성가능시촉사폐섬유화형성적인소지일。
AIM: To observe the kinetic alteration of nitric oxide formation in the lungs in the development of pulmonary fibrosis in the rat. METHODS: The contents of hydroxyproline in the lungs, NO-2/NO-3 (nitrite/nitrate) in out-flowing and in-flowing pulmonary blood (OPB, IPB) were assayed on the day 7, 14, 21, 30 and 70 after intratracheal administration of bleomycin　A5 . The content of NO-2/NO-3 in supernatants of culture of the alveolar macrophages (AMs) and the amount of iNOS positive stain cells in lung tissue section were also observed in the rat on 14th day after-bleomycin A5 administration. RESULTS: The content of lung hydroxyproline had no change on the 7th day, increased on the 14th day (P＜0.05), increased significantly on the 21th day, 30th day and 70th day post-bleomycin A5 compared with control rats. On the 7th day and 14th day, the content of NO-2/NO-3 increased in OPB and decreased in IPB (P＜0.01). On the 21th day, the content of NO-2/NO-3 abated in OPB (P＞0.05) but still decreased in IPB (P＜0.01). On the 30th day and the 70th day, the NO-2/NO-3 level recovered both in OPB and IPB. AMs from rats on the 14th day post-bleomycin A5 showed significant elevation (P＜0.01) in NO-2/NO-3 level. The amount of iNOS positive stain cells increased in rats on the 14th day post-bleomycin A5. CONCLUSION: The amount of NO in the lungs was high in the initial phase of fibroproliferative reaction induced by bleomycin A5 ,and these might be associated with the enhanced ability of AMs to release NO and the increased amount of iNOS.