CAJ | 학술논문

背景:维生素D受体基因在内切酶Bsm Ⅰ,ApaⅠ,TaqⅠ作用下,呈限制性内切酶片段长度多态性,并且与骨密度密切相关,而骨密度的变化对骨质疏松起着重要作用,但维生素D受体基因多态性与骨密度、骨质疏松相关性尚无定论.目的:分析在中国汉族、维吾尔族、哈萨克族、蒙古族绝经后妇女中与骨密度密切相关的维生素D受体基因多态性分布规律.设计:对比观察.单位:解放军总医院老年医学研究所.对象:选择2002-01/2003-12在解放军总医院进行健康体检的汉族绝经后妇女179名,平均年龄(59±3)岁.选择2001-01/2003-12于解放军兰州军区乌鲁木齐总医院老年科进行健康体检的维吾尔族、哈萨克族、蒙古族绝经后妇女者122,63,112名,平均年龄分别为(56±4),(55±3),(57±3)岁.均对检测项目知情同意.方法:应用聚合酶链反应限制性片段长度多态性技术确定维生素D受体基因BsmⅠ基因型,分析汉族、维吾尔族、哈萨克族和蒙古族绝经后妇女维生素D受体基因BsmⅠ多态性分布频率,并与已知的美国、澳大利亚、法国和日本相应数据进行比较.计数资料差异比较采用χ2检验.主要观察指标:汉族、维吾尔族、哈萨克族和蒙古族绝经后妇女维生素D受体基因BsmⅠ多态性分布频率,以及该分布特点与美国、澳大利亚、法国和日本相应数据比较结果.结果:汉族、维吾尔族、哈萨克族和蒙古族绝经后妇女维生素D受体基因bb基因型频率分别为90.5%,69.67%,38.1%和50%,BB基因型频率分别为0,4.1%,6.35%和4.46%,汉族与维吾尔族、哈萨克族和蒙古族维生素D受体基因型频率分布比较,差异明显(P＜0.01).哈萨克族与欧美人种比较,维生素D受体基因型差异不明显,与日本、韩国人种差异明显(P＜0.01).结论:中国汉族与维吾尔族、哈萨克族和蒙古族绝经后妇女维生素D受体基因型多态性具明显种族差异性;哈萨克族维生素D受体基因型频率分布接近欧美人种,与日本、韩国人种差异明显. 揹景:維生素D受體基因在內切酶Bsm Ⅰ,ApaⅠ,TaqⅠ作用下,呈限製性內切酶片段長度多態性,併且與骨密度密切相關,而骨密度的變化對骨質疏鬆起著重要作用,但維生素D受體基因多態性與骨密度、骨質疏鬆相關性尚無定論.目的:分析在中國漢族、維吾爾族、哈薩剋族、矇古族絕經後婦女中與骨密度密切相關的維生素D受體基因多態性分佈規律.設計:對比觀察.單位:解放軍總醫院老年醫學研究所.對象:選擇2002-01/2003-12在解放軍總醫院進行健康體檢的漢族絕經後婦女179名,平均年齡(59±3)歲.選擇2001-01/2003-12于解放軍蘭州軍區烏魯木齊總醫院老年科進行健康體檢的維吾爾族、哈薩剋族、矇古族絕經後婦女者122,63,112名,平均年齡分彆為(56±4),(55±3),(57±3)歲.均對檢測項目知情同意.方法:應用聚閤酶鏈反應限製性片段長度多態性技術確定維生素D受體基因BsmⅠ基因型,分析漢族、維吾爾族、哈薩剋族和矇古族絕經後婦女維生素D受體基因BsmⅠ多態性分佈頻率,併與已知的美國、澳大利亞、法國和日本相應數據進行比較.計數資料差異比較採用χ2檢驗.主要觀察指標:漢族、維吾爾族、哈薩剋族和矇古族絕經後婦女維生素D受體基因BsmⅠ多態性分佈頻率,以及該分佈特點與美國、澳大利亞、法國和日本相應數據比較結果.結果:漢族、維吾爾族、哈薩剋族和矇古族絕經後婦女維生素D受體基因bb基因型頻率分彆為90.5%,69.67%,38.1%和50%,BB基因型頻率分彆為0,4.1%,6.35%和4.46%,漢族與維吾爾族、哈薩剋族和矇古族維生素D受體基因型頻率分佈比較,差異明顯(P＜0.01).哈薩剋族與歐美人種比較,維生素D受體基因型差異不明顯,與日本、韓國人種差異明顯(P＜0.01).結論:中國漢族與維吾爾族、哈薩剋族和矇古族絕經後婦女維生素D受體基因型多態性具明顯種族差異性;哈薩剋族維生素D受體基因型頻率分佈接近歐美人種,與日本、韓國人種差異明顯.
배경:유생소D수체기인재내절매Bsm Ⅰ,ApaⅠ,TaqⅠ작용하,정한제성내절매편단장도다태성,병차여골밀도밀절상관,이골밀도적변화대골질소송기착중요작용,단유생소D수체기인다태성여골밀도、골질소송상관성상무정론.목적:분석재중국한족、유오이족、합살극족、몽고족절경후부녀중여골밀도밀절상관적유생소D수체기인다태성분포규률.설계:대비관찰.단위:해방군총의원노년의학연구소.대상:선택2002-01/2003-12재해방군총의원진행건강체검적한족절경후부녀179명,평균년령(59±3)세.선택2001-01/2003-12우해방군란주군구오로목제총의원노년과진행건강체검적유오이족、합살극족、몽고족절경후부녀자122,63,112명,평균년령분별위(56±4),(55±3),(57±3)세.균대검측항목지정동의.방법:응용취합매련반응한제성편단장도다태성기술학정유생소D수체기인BsmⅠ기인형,분석한족、유오이족、합살극족화몽고족절경후부녀유생소D수체기인BsmⅠ다태성분포빈솔,병여이지적미국、오대리아、법국화일본상응수거진행비교.계수자료차이비교채용χ2검험.주요관찰지표:한족、유오이족、합살극족화몽고족절경후부녀유생소D수체기인BsmⅠ다태성분포빈솔,이급해분포특점여미국、오대리아、법국화일본상응수거비교결과.결과:한족、유오이족、합살극족화몽고족절경후부녀유생소D수체기인bb기인형빈솔분별위90.5%,69.67%,38.1%화50%,BB기인형빈솔분별위0,4.1%,6.35%화4.46%,한족여유오이족、합살극족화몽고족유생소D수체기인형빈솔분포비교,차이명현(P＜0.01).합살극족여구미인충비교,유생소D수체기인형차이불명현,여일본、한국인충차이명현(P＜0.01).결론:중국한족여유오이족、합살극족화몽고족절경후부녀유생소D수체기인형다태성구명현충족차이성;합살극족유생소D수체기인형빈솔분포접근구미인충,여일본、한국인충차이명현.
BACKGROUND: Vitamin D receptor (VDR) gene shows restriction fragment length polymorphism with incision enzyme Bsm Ⅰ ,Apa Ⅰ ,Taq Ⅰ ,which is related to bone mineral density (BMD).However, it is unclear that the relationship between VDR gene (Bsm Ⅰ ) polymorphisms and BMD,osteoporosis.OBJECTIVE: To analyze the distribution regularity of vitamin D receptor gene polymorphism related to BMD in postmenopausal women of Han,Uygur, Kazak and Mongoloid nationality in China. DESIGN: controlled observation.SETTING: Institute of Gerontology,General Hospital of Chinese PLA.PARTICIPANTS: Totally 179 women of Han,who were taking physical examination in General Hospital of Chinese PLA from January 2002 to December 2003, at the average age of (59±3) years,were selected. A total number of 122 women of Uygur with average age of 56.49 years; 63 women of Kazak with average age of (55±3) years; and 112 women of Mongoloid with average age of (57±3) years,who were all taking physical examination in department of geriatrics, Urumqi General Hospital of Lanzhou Military Area Command of Chinese PLA from January 2001 to December 2003.All of them were informed consent.METHODS: VDR genotypes(Bsm Ⅰ ) were defined with polymerase chain reaction-restriction fragment length polymorphism,so as to analyze distribution of Vitamin-D receptor gene (Bsm Ⅰ )polymorphisms of postmenopausal women in Han,Uygur, Kazak,Mongoloid nationality,and compared with the data of USA,Australia,France,Japan,Korea. Enumeration data were compared with Chi-square test.MAIN OUTCOME MEASURES: VDR (Bsm Ⅰ ) polymorphisms in healthy postmenopausal women from Han, Uygur, Kazak, Mongoloid populations in China, which were compared with the data of USA, Australia,France, Japan, Korea.RESULTS: For women of Han, Uygur, Kazak and Mongoloid nationality,the BB genotypes accounted for 0, 4.1%, 6.35% and 4.46%, the bb genotypes accounted for 90.5%, 69.67%, 38.1% and 50% respectively. There was a significant difference between women of Han, Uygur, Kazak, and Mongoloid nationality(P ＜ 0.01). There was insignificant difference in comparation of distribtuion of VDR genotype between Kazak nationality and the west races, but it was significantly different to that in Japan,Korea races.CONCLUSION:VDR genotype polymorphisms is characterized by obvious racial diversify in postmenopausal women of Han,Uygur, Kazak,Mongoloid populations in China;Distribution of VDR gene frequency of Kazak population is similar with the west race ,but is different to Japanese and Korea's race.