菌物研究
菌物研究
균물연구
JOURNAL OF FUNGAL RESEARCH
2008年
1期
57-62
,共6页
地衣%喇叭石蕊%共生菌%共生藻%液体培养
地衣%喇叭石蕊%共生菌%共生藻%液體培養
지의%나팔석예%공생균%공생조%액체배양
lichen%Cladonia pyxidata%mycobiont%photobiont%liquid culture
对喇叭石蕊共生菌、藻液体培养条件进行了研究.结果表明:共生菌生长在以40 g/L肌醇为碳源、2g/L L-谷氯酰胺为氮源、起始pH值为7.0的LB液体培养基中,培养温度为20 ℃时表现最佳.其共生藻的生长在以160 g/L葡萄糖为碳源、1.75 g/L NaNO3为氮源、起始pH值为5.0的BBM液体培养基中,培养温度为20℃时表现最佳.
對喇叭石蕊共生菌、藻液體培養條件進行瞭研究.結果錶明:共生菌生長在以40 g/L肌醇為碳源、2g/L L-穀氯酰胺為氮源、起始pH值為7.0的LB液體培養基中,培養溫度為20 ℃時錶現最佳.其共生藻的生長在以160 g/L葡萄糖為碳源、1.75 g/L NaNO3為氮源、起始pH值為5.0的BBM液體培養基中,培養溫度為20℃時錶現最佳.
대나팔석예공생균、조액체배양조건진행료연구.결과표명:공생균생장재이40 g/L기순위탄원、2g/L L-곡록선알위담원、기시pH치위7.0적LB액체배양기중,배양온도위20 ℃시표현최가.기공생조적생장재이160 g/L포도당위탄원、1.75 g/L NaNO3위담원、기시pH치위5.0적BBM액체배양기중,배양온도위20℃시표현최가.
This paper deals with the cell growth of both the mycohiont and photobiont of Cladonia pyxidata collected from China in liquid culture.The experiments results showed that the better conditions of mycobiontic growth were cultured by using liquid Lilly-Barnett medium containing 40 g/L inositol as a carbon source,2 g/L L-glutamine as a nitrogen source,adjusting pH to 7.0 before autoclaving,and incubating cultures at 20℃.The better conditions of photobiontic growth were cultured by using liquid Bold's basal medium containing 160 g/L glucose as a carbon source,1.75 g/L NaNO3 as a nitrogen source,adjusting pH to 5.0 before autoclaving,and incubating cultures at 20℃.
