CAJ | 학술논문

通过RACE和RT-PCR方法从番茄中克隆了LeEBF1(EIN3 binding F-box protein 1)和LeEBF3(EIN3 binding F-box protein 2)的全长cDNA序列,两个基因LeEBF1、LeEBF2全长分别是2866和2 891 bp,对序列的分析表明,它们的开放阅读框分别是1 911和1 995 bp.编码区编码637和665个氨基酸残基,在氨基端含保守的F-box区域和在羧基端有14个亮氨酸重复单位,通过BLAST软件和DNAMAN分析表明这两个基因的氨基酸序列与拟南芥EBF1和EBF2有58.6%相似,同时又与其他物种的EBF蛋白的F-box区域比较有24.4%到73.2%的相近.Northern杂交指出:LeEBF1与LeEBF2在野生型和Nr的幼叶中的表达量高于成熟叶;当在果实发育期,LeEBF1与LeEBF2在青果期的表达量相比其他时期要弱.初步结果表明,LeEBF1与LeEBF2可能在番茄的生长发育中起着重要的作用.
통과RACE화RT-PCR방법종번가중극륭료LeEBF1(EIN3 binding F-box protein 1)화LeEBF3(EIN3 binding F-box protein 2)적전장cDNA서렬,량개기인LeEBF1、LeEBF2전장분별시2866화2 891 bp,대서렬적분석표명,타문적개방열독광분별시1 911화1 995 bp.편마구편마637화665개안기산잔기,재안기단함보수적F-box구역화재최기단유14개량안산중복단위,통과BLAST연건화DNAMAN분석표명저량개기인적안기산서렬여의남개EBF1화EBF2유58.6%상사,동시우여기타물충적EBF단백적F-box구역비교유24.4%도73.2%적상근.Northern잡교지출:LeEBF1여LeEBF2재야생형화Nr적유협중적표체량고우성숙협;당재과실발육기,LeEBF1여LeEBF2재청과기적표체량상비기타시기요약.초보결과표명,LeEBF1여LeEBF2가능재번가적생장발육중기착중요적작용.
Two full-length cDNAs of LeEBF1 (EIN3 binding F-box protein 1)and LeEBF2(EIN3 bind-ing F-box protein 2)were cloned from tomato (Solanum lycopersicum) by RT-PCR and rapid amplifica-tion of eDNA ends (RACE). They were 2 866 and 2 891 bp, with open reading frame (ORF) of 1 911 bp and 1 995 bp, encoding 637 and 665 amino acids, respectively. Putative LeEBF1 and LeEBF2 pro-teins, each containing a well-conserved F-box motif in the amino terminus and 14 tandem leucine-rich repeats (LRRs) in the carboxyl terminus, shared 58.6% of identity with each other. Identities with other species EBF proteins within F box motif ranged from 24.4% to 73.2%. Northern blot analysis re-sults indicated that the expression levels of LeEBF1 and LeEBF2 in young leaves were higher than those in mature leaves of wild type and Nr. During the development of fruits, LeEBF1 and LeEBF2 expressed relatively weaker at IMG stage than at other stages tested. In a conclusion, LeEBF1 and LeEBF2 may play an important role in leaf and fruit development of tomato.