CAJ | 학술논문

非接触电导检测法已经成为毛细管电泳芯片中一种通用的物质分离检测方法.本文提出了一种微型化的电容耦合非接触电导分析系统,采用新颖的三明治电极结构减小了电极之间的寄生电容.这种电极结构能够有效地减小电极的长度,克服了非接触电导检测中电极易于折断的缺点,当采用电极宽度1 mm、电极间距1 mm、MES/His缓冲溶液浓度为20mmol/L和激励电压20Vpp、90 kHz的条件时,检测器具有最好的分离效果.在最佳的分离条件下,实现了1mmol/L的K+和Mg2+混合无机阳离子的分离检测.
비접촉전도검측법이경성위모세관전영심편중일충통용적물질분리검측방법.본문제출료일충미형화적전용우합비접촉전도분석계통,채용신영적삼명치전겁결구감소료전겁지간적기생전용.저충전겁결구능구유효지감소전겁적장도,극복료비접촉전도검측중전겁역우절단적결점,당채용전겁관도1 mm、전겁간거1 mm、MES/His완충용액농도위20mmol/L화격려전압20Vpp、90 kHz적조건시,검측기구유최호적분리효과.재최가적분리조건하,실현료1mmol/L적K+화Mg2+혼합무궤양리자적분리검측.
Contactless conductivity detection has become an accepted detection method in capillary electrophoresis chip for a variety of analytes. In this paper, a miniaturized analytical system with capacitively coupled contactless conductivity detection was presented. Polymethyl methacrylate (PMMA) was used as substrate to substitute for glass material. A novel sandwich electrode structure was used to minimize the stray capacitance of the detector. The construction can decrease the length of the electrode and overcomes the disadvantage of electrode frangibility. Experimental parameters relevant to separation and detection processes were optimized to separate the mixture of inorganic cations K+ and Mg2+ (1 mmol/L) with electrodes of 1 mm width, 20 mmol/L MES/His buffer solution and a detection gap of 1 mm when operating the detector at 20Vpp and 90 kHz.