时珍国医国药
時珍國醫國藥
시진국의국약
LISHIZHEN MEDICINE AND MATERIA MEDICA RESEARCH
2009年
7期
1707-1709
,共3页
罗祖友%陈根洪%郑小江%吴谋成
囉祖友%陳根洪%鄭小江%吳謀成
라조우%진근홍%정소강%오모성
藤茶%多糖%分离纯化%结构鉴定
籐茶%多糖%分離純化%結構鑒定
등다%다당%분리순화%결구감정
Ampelopsis grossedentata%Polysaccharide%Isolation and purification%Structure identification
目的 对藤茶水溶性多糖进行分离纯化得到AGP-3组分,并对其结构进行初步的鉴定,为藤茶多糖结构与生物活性关系的研究奠定基础.方法 采用DEAE-Sephadex A-25、Sephadex G-200和HPLC对藤茶多糖分离纯化,并通过IR、GC、1HNMR和13CNMR分析对藤茶多糖AGP-3组分的结构进行了初步鉴定.结果 AGP-3重均分子量(Mw)为1.02×105 Da,含中性糖质量分数57.6%、糖醛酸32.3%、蛋白质3.5%.结论 藤茶多糖分离组分AGP-3为一种含蛋白质的复合多糖.
目的 對籐茶水溶性多糖進行分離純化得到AGP-3組分,併對其結構進行初步的鑒定,為籐茶多糖結構與生物活性關繫的研究奠定基礎.方法 採用DEAE-Sephadex A-25、Sephadex G-200和HPLC對籐茶多糖分離純化,併通過IR、GC、1HNMR和13CNMR分析對籐茶多糖AGP-3組分的結構進行瞭初步鑒定.結果 AGP-3重均分子量(Mw)為1.02×105 Da,含中性糖質量分數57.6%、糖醛痠32.3%、蛋白質3.5%.結論 籐茶多糖分離組分AGP-3為一種含蛋白質的複閤多糖.
목적 대등다수용성다당진행분리순화득도AGP-3조분,병대기결구진행초보적감정,위등다다당결구여생물활성관계적연구전정기출.방법 채용DEAE-Sephadex A-25、Sephadex G-200화HPLC대등다다당분리순화,병통과IR、GC、1HNMR화13CNMR분석대등다다당AGP-3조분적결구진행료초보감정.결과 AGP-3중균분자량(Mw)위1.02×105 Da,함중성당질량분수57.6%、당철산32.3%、단백질3.5%.결론 등다다당분리조분AGP-3위일충함단백질적복합다당.
Objective To provide a basis for the research about the relation between the structure and its bioactivities of polysaccharides from Ampelopsis grossedentata.The polysaccharide fraction AGP-3 was obtained and analyzed preliminarily after water-soluble polysaccharides from Ampelopsis grossedentata being isolated and purified.Methods The water-soluble polysaccharides from Ampelopsis grossedentata were isolated and purified with DEAE-Sephadex A-25,Sephadex G-200 and HPLC,and the structure of AGP-3 was analyzed preliminarily by IR,GC,1HNMR and 13CNMR.Results The molecular average-weight (MW) of AGP-3 was 1.02×105 Da,containing 57.6% neutral sugar,32.3% uronic acid and 3.5% protein.Conclusion The polysaccharide fraction AGP-3 from Ampelopsis grossedentata is a kind of conjugated polysaccharide containing protein.
