CAJ | 학술논문

基于不同pH下维生素C和扑热息痛的光谱差异,将顺序注射pH梯度技术与人工神经网络算法相结合,用于两种药物成分重叠光谱的解析,实现了两种药物成分的同时测定.实验中讨论了顺序注射pH梯度的建立条件,考察了NaOH浓度、NaOH体积、检测流速等反应条件的影响.在选定的实验条件下,维生素C和扑热息痛的测定范围为1.0～10.0 mg/L,检出限分别为0.3和0.2 mg/L,分析频率为40样/h.将该方法用于维C银翘片中维生素C和扑热息痛的同时测定,分析结果与标示量无显著性差异,加标回收率在92.0%～109.1%之间.
기우불동pH하유생소C화복열식통적광보차이,장순서주사pH제도기술여인공신경망락산법상결합,용우량충약물성분중첩광보적해석,실현료량충약물성분적동시측정.실험중토론료순서주사pH제도적건립조건,고찰료NaOH농도、NaOH체적、검측류속등반응조건적영향.재선정적실험조건하,유생소C화복열식통적측정범위위1.0～10.0 mg/L,검출한분별위0.3화0.2 mg/L,분석빈솔위40양/h.장해방법용우유C은교편중유생소C화복열식통적동시측정,분석결과여표시량무현저성차이,가표회수솔재92.0%～109.1%지간.
A sequential injection pH gradient spectrophotometric method is proposed for simultaneous determination of vitamic C and paracetamol in pharmaceutials. The method is based on the difference between absorption spectra of vitamin C and paracetamol at different pH. The pH gradient in the range of 9.2～10.9 was establised by sequential injection of NaOH solution in a flow system. Artificial neural network algorithms were applied to resolving overlapping spectra of components and simultaneous determination of their concentrations. Experimental variables were investigated, including NaOH concentration, NaOH and sample volumes, and flow rate. Under selected experimental conditions, the linear range was 1.0～10.0mg/L for vitamin C and paracetamol, with detection limits of 0.3mg/L and 0.2mg/L, respectively. The sampling frequency was 40/h. The method has been used to the determination of vitamin C and paracetamol in vitamin C yinqiao tablets, and the results were not statistically different to the claimed values, and the recoveries for all components were in the range of 92.0%～109.1%.