中国预防兽医学报
中國預防獸醫學報
중국예방수의학보
CHINESE JOURNAL OF PREVENTIVE VETERINARY MEDICINE
2010年
4期
276-280
,共5页
万晓朋%曾显营%田国彬%李雁冰%邓国华%施建忠%刘丽玲%钟功勋%姜永萍%王彦妮%陈化兰
萬曉朋%曾顯營%田國彬%李雁冰%鄧國華%施建忠%劉麗玲%鐘功勛%薑永萍%王彥妮%陳化蘭
만효붕%증현영%전국빈%리안빙%산국화%시건충%류려령%종공훈%강영평%왕언니%진화란
禽流感%H9N2亚型%灭活疫苗%种毒%筛选
禽流感%H9N2亞型%滅活疫苗%種毒%篩選
금류감%H9N2아형%멸활역묘%충독%사선
Avian influenza%H9N2 subtype%inactivated vaccine%vaccine candidate%selection
为筛选出具有良好免疫原性的禽流感病毒(AIV)H9N2亚型灭活流行株种毒,选择2008年中国大陆8个省份15株H9N2亚型AIV分离株进行抗原性分析,选取代表流行株进行鉴定并制备灭活疫苗,进行免疫效力评估.实验结果显示,2008年分离株之间抗原性比较接近,与2000年前分离株的抗原性相差较大;2008年分离的8株病毒HA基因核苷酸同源率在93.2%～98.6%之间,而CK/SH/10/01毒株与这8株病毒的同源率仅在91.9%～93.5%之间:CK/ZJ/17/08、CK/SD/2CZ/08、DK/FJ/560/08、CK/FJ/521/08、CK/HuN/174/08和CK/HuN/33/08候选株病毒在SPF鸡胚上连续传15代HA价及致病性等均未改变,SPF鸡鼻腔感染106 EID50各毒株后均无任何症状和死亡出现;候选株灭活疫苗免疫SPF鸡3周时,产生针对疫苗株抗原检测的HI抗体介于10.35 log2～11.811 log2,以106 EID50剂量鼻腔感染途径攻毒后,只有CK/HuN/174/08和CK/HuN/33/08株灭活疫苗免疫鸡不仅可以对同源毒株的攻击提供良好的免疫保护,而且对2008年分离的异源毒株的攻击也能提供比较理想的免疫保护,可作为适合我国大部分地区应用的H9N2亚型禽流感灭活疫苗种毒株.
為篩選齣具有良好免疫原性的禽流感病毒(AIV)H9N2亞型滅活流行株種毒,選擇2008年中國大陸8箇省份15株H9N2亞型AIV分離株進行抗原性分析,選取代錶流行株進行鑒定併製備滅活疫苗,進行免疫效力評估.實驗結果顯示,2008年分離株之間抗原性比較接近,與2000年前分離株的抗原性相差較大;2008年分離的8株病毒HA基因覈苷痠同源率在93.2%～98.6%之間,而CK/SH/10/01毒株與這8株病毒的同源率僅在91.9%～93.5%之間:CK/ZJ/17/08、CK/SD/2CZ/08、DK/FJ/560/08、CK/FJ/521/08、CK/HuN/174/08和CK/HuN/33/08候選株病毒在SPF鷄胚上連續傳15代HA價及緻病性等均未改變,SPF鷄鼻腔感染106 EID50各毒株後均無任何癥狀和死亡齣現;候選株滅活疫苗免疫SPF鷄3週時,產生針對疫苗株抗原檢測的HI抗體介于10.35 log2～11.811 log2,以106 EID50劑量鼻腔感染途徑攻毒後,隻有CK/HuN/174/08和CK/HuN/33/08株滅活疫苗免疫鷄不僅可以對同源毒株的攻擊提供良好的免疫保護,而且對2008年分離的異源毒株的攻擊也能提供比較理想的免疫保護,可作為適閤我國大部分地區應用的H9N2亞型禽流感滅活疫苗種毒株.
위사선출구유량호면역원성적금류감병독(AIV)H9N2아형멸활류행주충독,선택2008년중국대륙8개성빈15주H9N2아형AIV분리주진행항원성분석,선취대표류행주진행감정병제비멸활역묘,진행면역효력평고.실험결과현시,2008년분리주지간항원성비교접근,여2000년전분리주적항원성상차교대;2008년분리적8주병독HA기인핵감산동원솔재93.2%～98.6%지간,이CK/SH/10/01독주여저8주병독적동원솔부재91.9%～93.5%지간:CK/ZJ/17/08、CK/SD/2CZ/08、DK/FJ/560/08、CK/FJ/521/08、CK/HuN/174/08화CK/HuN/33/08후선주병독재SPF계배상련속전15대HA개급치병성등균미개변,SPF계비강감염106 EID50각독주후균무임하증상화사망출현;후선주멸활역묘면역SPF계3주시,산생침대역묘주항원검측적HI항체개우10.35 log2～11.811 log2,이106 EID50제량비강감염도경공독후,지유CK/HuN/174/08화CK/HuN/33/08주멸활역묘면역계불부가이대동원독주적공격제공량호적면역보호,이차대2008년분리적이원독주적공격야능제공비교이상적면역보호,가작위괄합아국대부분지구응용적H9N2아형금류감멸활역묘충독주.
In this study, candidate vaccine strains were selected from fifteen H9N2 subtype avian influenza viruses (AIV) isolated from eight provinces in mainland China during 2008. Six vaccine strains including CK/ZJ/17/08, CK/SD/2CZ/08, DK/FJ/560/08, CK/FJ/521/08, CK/HuN/174/08 and CK/HuN/33/08 were selected based on the antigenic analysis. The immune efficacy of the strains was evaluated by inoculaing SPF chickens with oil-emulsion inactivated vaccines prepared from respective strains. The results showed that all vaccine strains could induce high level (10.35 to 11.81 log2) hemagglutination inhibition antibody against homologous H9N2 virus at 3 weeks post-immunization, and the CK/HuN/174/08 and CK/HuN/33/08 inactivated vaccines were shown to protect the SPF chickens from challenge by both homologous and heterologous AIV H9N2 strain. The results indicated that only CK/HuN/174/08 and CK/HuN/33/08, but not all the H9N2 strains, have the potential attributes to be qualified as inactivated vaccine candidates against H9N2 virus in most parts of mainland China.
