中华病理学杂志
中華病理學雜誌
중화병이학잡지
Chinese Journal of Pathology
2009年
3期
178-182
,共5页
玄延花%李贵铃%姜宏宇%林贞花
玄延花%李貴鈴%薑宏宇%林貞花
현연화%리귀령%강굉우%림정화
子宫肿瘤%乳头状瘤病毒感染%宫颈上皮内瘤样病变
子宮腫瘤%乳頭狀瘤病毒感染%宮頸上皮內瘤樣病變
자궁종류%유두상류병독감염%궁경상피내류양병변
Uterine neoplasms%Papillomavirns infections%Cervical intraepithelial neoplasia
目的 探讨Hedgehog(Hh)信号通路蛋白在宫颈癌及其癌前病变中的临床病理学意义,并分析其与人乳头状瘤病毒(HPV)16型感染的关系.方法 32例正常宫颈上皮、71例宫颈上皮内瘤变(CIN;CIN Ⅰ 28例,CIN Ⅱ 18例,CIN Ⅲ25例)和80例宫颈鳞状细胞癌共183例选自延边大学医院、延边妇幼保健院和延边肿瘤医院病理科存档蜡块.应用PCR技术检测上述组织中HPV16型的感染情况,并应用Shh、Ihh、Ptch和Smo 4种Hh信号通路蛋白抗体、组织芯片和免疫组织化学EnVision法检测Hh信号通路蛋白在上述病变组织中的表达情况.结果 Shh、Ihh、Ptch和Smo在正常宫颈黏膜上皮中为弱阳性,而在宫颈癌和CIN Ⅲ中呈强阳性,其表达率均显著高于正常宫颈黏膜上皮(P均<0.05).80例官颈癌标本中HPV16阳性率是77.5%(62/80),而且Shh蛋白的强阳性率在HPV16型阳性的宫颈癌组织中显著高于HPV16阴性组(P<0.05).结论 Hh信号通路蛋白过表达可以作为宫颈癌及其癌前病变的早期辅助诊断指标并有望成为宫颈癌靶向治疗的新靶点,而且Shh蛋白的过表达与HPV16型感染密切相关.
目的 探討Hedgehog(Hh)信號通路蛋白在宮頸癌及其癌前病變中的臨床病理學意義,併分析其與人乳頭狀瘤病毒(HPV)16型感染的關繫.方法 32例正常宮頸上皮、71例宮頸上皮內瘤變(CIN;CIN Ⅰ 28例,CIN Ⅱ 18例,CIN Ⅲ25例)和80例宮頸鱗狀細胞癌共183例選自延邊大學醫院、延邊婦幼保健院和延邊腫瘤醫院病理科存檔蠟塊.應用PCR技術檢測上述組織中HPV16型的感染情況,併應用Shh、Ihh、Ptch和Smo 4種Hh信號通路蛋白抗體、組織芯片和免疫組織化學EnVision法檢測Hh信號通路蛋白在上述病變組織中的錶達情況.結果 Shh、Ihh、Ptch和Smo在正常宮頸黏膜上皮中為弱暘性,而在宮頸癌和CIN Ⅲ中呈彊暘性,其錶達率均顯著高于正常宮頸黏膜上皮(P均<0.05).80例官頸癌標本中HPV16暘性率是77.5%(62/80),而且Shh蛋白的彊暘性率在HPV16型暘性的宮頸癌組織中顯著高于HPV16陰性組(P<0.05).結論 Hh信號通路蛋白過錶達可以作為宮頸癌及其癌前病變的早期輔助診斷指標併有望成為宮頸癌靶嚮治療的新靶點,而且Shh蛋白的過錶達與HPV16型感染密切相關.
목적 탐토Hedgehog(Hh)신호통로단백재궁경암급기암전병변중적림상병이학의의,병분석기여인유두상류병독(HPV)16형감염적관계.방법 32례정상궁경상피、71례궁경상피내류변(CIN;CIN Ⅰ 28례,CIN Ⅱ 18례,CIN Ⅲ25례)화80례궁경린상세포암공183례선자연변대학의원、연변부유보건원화연변종류의원병이과존당사괴.응용PCR기술검측상술조직중HPV16형적감염정황,병응용Shh、Ihh、Ptch화Smo 4충Hh신호통로단백항체、조직심편화면역조직화학EnVision법검측Hh신호통로단백재상술병변조직중적표체정황.결과 Shh、Ihh、Ptch화Smo재정상궁경점막상피중위약양성,이재궁경암화CIN Ⅲ중정강양성,기표체솔균현저고우정상궁경점막상피(P균<0.05).80례관경암표본중HPV16양성솔시77.5%(62/80),이차Shh단백적강양성솔재HPV16형양성적궁경암조직중현저고우HPV16음성조(P<0.05).결론 Hh신호통로단백과표체가이작위궁경암급기암전병변적조기보조진단지표병유망성위궁경암파향치료적신파점,이차Shh단백적과표체여HPV16형감염밀절상관.
Objective To investigate the significance of sonic hedgehog (Shh), indian hedgehog (Ihh), smoothened (Smo) and patched (Ptch) expressions in uterine cervical lesions and their relationships with HPV type 16 infection. Methods Totally 183 cases of cervical lesions, including 32 non-neoplastic cervix, 71 cervical intraepithelial neoplasia (28 CIN Ⅰ , 18 CIN Ⅱ, and 25 CIN Ⅲ) and 80 squamous cell carcinomas (SCC) were selected from the Department of Pathology, Yanbian University Hospital, Yanbian Women Hospital, and Yanhian Tumor Hospital. Shh, Ihh, Ptch and Smo proteins expression were investigated by immunohistochemistry using tissue microarry platform, and the presence of HPV type 16 was detected by PCR method. Results Immunohistochemical staining showed that the frequencies of Shh, Ihh, Pteh and Smo expression were rare in normal cervical epithelium, but were strongly expressed in cervical cancer and its precursor lesions ( CIN Ⅱ/Ⅲ) (P<0.01, P<0.01, P<0.05, P<0.05, respectively). In cervical cancer, the expression rate of Shh (95%) was higher than that of CIN (CIN Ⅰ to CIN Ⅲ) (46.4%, 61.1%, 80.0%, respectively, P<0.05). HPV16 was positive in 77.5% of SCC. In cervical cancer, the expression of Shh was related with HPV16 infection (P<0.05), and the expression of Smo was correlated with lymph node metastasis (P<0.05). Conclusions Shh, Ihh, Ptch,and Smo genes may play important roles in the development of cervical cancer. Detection of Hedgehog signaling pathway molecules seems helpful for the early diagnosis of cervical cancer and its precursor lesions,and are potentially therapeutic targets as well.
