CAJ | 학술논문

目的探讨γ-连环蛋白、过氧化物酶体增殖物激活受体γ(PPARγ)在肝癌组织中的表达及意义.方法利用组织芯片技术构建肝癌组织芯片,采用免疫组织化学SP法检测49例肝癌组织及相应癌旁组织、6例正常肝组织中β-连环蛋白、PPART蛋白的表达,分析PPARγ与分化程度等临床和病理指标的关系及β-连环蛋白与PPARγ的关系.结果 49例肝癌组织中β-catenin异常表达34例(69.39%);癌旁组织中异常表达24例(48.98%);6例正常肝组织中表达均正常;差异有统计学意义(精确概率法,P=0.001).49例肝癌组织中PPARγ表达阳性25例(51.02%),癌旁组织中15例阳性(30.61%),6例正常肝组织中未见表达,差异有统计学意义(精确概率法,P=0.016).肝癌患者不同年龄、肿瘤大小、血清甲胎蛋白水平、门/腔静脉癌栓有无、HBsAg感染与否的PPAR-γ表达程度差异无统计学意义(χ2值分别为0.214、3.201、0.046、3.201,P均>0.05);不同分化程度的PPARγ表达程度差异有统计学意义(χ22=4.693,P<0.05).β-catenin异常表达与PPARγ阳性表达相关(χ2=5.130,P<0.05).结论异常表达的β-catenin可能参与肝癌的形成.PPARγ蛋白在肝癌组织中呈高表达,其表达与肝癌病理分化有关,检测PPARγ蛋白对诊断肝癌、判断其恶性程度及预后均有一定参考价值.
목적 탐토γ-련배단백、과양화물매체증식물격활수체γ(PPARγ)재간암조직중적표체급의의.방법 이용조직심편기술구건간암조직심편,채용면역조직화학SP법검측49례간암조직급상응암방조직、6례정상간조직중β-련배단백、PPART단백적표체,분석PPARγ여분화정도등림상화병리지표적관계급β-련배단백여PPARγ적관계.결과 49례간암조직중β-catenin이상표체34례(69.39%);암방조직중이상표체24례(48.98%);6례정상간조직중표체균정상;차이유통계학의의(정학개솔법,P=0.001).49례간암조직중PPARγ표체양성25례(51.02%),암방조직중15례양성(30.61%),6례정상간조직중미견표체,차이유통계학의의(정학개솔법,P=0.016).간암환자불동년령、종류대소、혈청갑태단백수평、문/강정맥암전유무、HBsAg감염여부적PPAR-γ표체정도차이무통계학의의(χ2치분별위0.214、3.201、0.046、3.201,P균>0.05);불동분화정도적PPARγ표체정도차이유통계학의의(χ22=4.693,P<0.05).β-catenin이상표체여PPARγ양성표체상관(χ2=5.130,P<0.05).결론 이상표체적β-catenin가능삼여간암적형성.PPARγ단백재간암조직중정고표체,기표체여간암병리분화유관,검측PPARγ단백대진단간암、판단기악성정도급예후균유일정삼고개치.
Objective To investigate the expression and significance of β-catenin and peroxisome prolifera-tot-activated receptor-γ,(PPARγ) in bepatocellular carcinoma. Methods Tissue microarrays were established to detect β-catenin and PPARγ expression in 49 cases of hepatocellular carcinoma,49 cases of adjacent nontumoral liv-er tissue and 6 cases of normal liver tissue. The relationships between PPARγ and β-catenin as well as between PPARγ and clinicopathological parameters were observed. Results The aberrant expression rate of β-catenin was 69.39%,48.98 % and 0 respectively (P=0.001). The positive expression rate of PPARγ was 51.02%,30.61% and 0 respectively (P=0.016). Clinicopathological analysis revealed that the increase of PPARγ expression was not associated with age,tumor size,serum alpha fetoprotein (AFP) levels,tumor embolus of portal vein or inferior vena cava,and HBsAg infection(χ2=0.214,3.201,0.046,3.201,P>0.05 for each),but correlated with differentiation grades(χ2=4.693,P<0.05). Aberrant expression of β-catenin was associated with PPARγ expression(χ2= 5.130,P<0.05). Conclusion Aberrant expression of β-catenin may involve in the liver carcinogenesis. The high expression of PPARγ in hepatocellular carcinoma is significantly correlated with the clinicopathological characteris-tics. Detection of PPARγ is valuable for diagnosing hepatocellular carcinoma,and evaluating malignancy extent and prognosis.