CAJ | 학술논문

目的观察-1195G>A单核苷酸多态对胰腺癌细胞中COX-2基因启动子转录活性的影响.方法采用PCR-RFLP对COX-2基因-1195位点各基因型进行分型;构建COX-2基因启动子区pA-1195和pG-1195荧光素酶报告基因表达质粒;转染胰腺癌细胞系Asp-1;采用双荧光素酶报告基因方法,检测-1195AA和-1195GG基因型对报告基因表达的影响.结果与含-1195GG基因型的COX-2启动子相比,含-1195AA基因型的COX-2启动子,在胰腺癌细胞Aspc-1具有较高的报告基因表达水平.重组质粒pA1195驱动的报告基因表达水平为pG1195驱动的报告基因表达水平的17.83倍.结论 COX-2基因启动子区的-1195G>A单核苷酸多态-1195位点G→A改变明显增加胰腺癌细胞COX-2基因启动子的转录活性.
목적 관찰-1195G>A단핵감산다태대이선암세포중COX-2기인계동자전록활성적영향.방법 채용PCR-RFLP대COX-2기인-1195위점각기인형진행분형;구건COX-2기인계동자구pA-1195화pG-1195형광소매보고기인표체질립;전염이선암세포계Asp-1;채용쌍형광소매보고기인방법,검측-1195AA화-1195GG기인형대보고기인표체적영향.결과 여함-1195GG기인형적COX-2계동자상비,함-1195AA기인형적COX-2계동자,재이선암세포Aspc-1구유교고적보고기인표체수평.중조질립pA1195구동적보고기인표체수평위pG1195구동적보고기인표체수평적17.83배.결론 COX-2기인계동자구적-1195G>A단핵감산다태-1195위점G→A개변명현증가이선암세포COX-2기인계동자적전록활성.
Objective To investigate the effects of single nucleotide polymorphism of-1195G.>A in the promoter of COX-2 gene on the transcriptional activity of COX-2 gene in the pancreatic cancer cell line. Methods The genotypes of-1195AA and-1195GG of COX-2 gene were deter-mined by polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP).The reporter plasmids with-1195AA and-1195GG genotypes were constructed and then the pan-creatic Aspc-lcancer cells were transfected with these two kinds of plasmaids. The transcriptional ac-tivity of the COX-2 promoter in Aspc-1 transfected with-1195AA or-1195GG genotype was tested by luciferase report gene assay. Results The transcriptional activity of the COX-2 promoter in Aspc-1 transfected with-1195AA genotype was as 17.83 times higher as that with-1195GG genotype.Conclusion The single nucleotide polymorphism of-1195G.>A can greatly up-regulate the tran-scriptional activity of the promoter of COX-2 gene in the pancreatic cancer cells.