中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2011年
6期
430-433
,共4页
肿瘤,鳞状细胞%钾通道,电压门控%角蛋白细胞
腫瘤,鱗狀細胞%鉀通道,電壓門控%角蛋白細胞
종류,린상세포%갑통도,전압문공%각단백세포
Neoplasms,squamous cell%Potassium channels,voltage-gated%Keratinocytes
目的 探讨电压门控钾通道在人皮肤鳞状细胞癌A431细胞和人角质形成细胞HaCaT细胞中的作用.方法 噻唑蓝方法研究加入含有不同浓度四乙胺的培养基对A431细胞和HaCaT细胞的增殖的影响;提取体外培养A431细胞、HaCaT细胞以及人正常表皮组织的蛋白,ELISA方法检测电压门控钾通道蛋白(HERG)在它们中的表达,比较它们的差异.结果 四乙胺对体外培养的A431细胞和HaCaT细胞的增殖抑制效应具有时间依赖与剂量依赖的特点,当四乙胺的浓度≥10 mmol/L且作用时间≥24 h,可以使A431细胞和HaCaT细胞的增殖受到明显的抑制.HERG钾通道蛋白在A431细胞、HaCaT细胞和人正常皮肤表皮组织均有表达,平均浓度分别为(49.7114±3.55696)pg/ml、(35.7471±4.14696)pg/ml、(36.8857±3.47810)pg/ml,组间比较差异具有统计学意义(P<0.05).结论 阻断电压门控钾通道可抑制A431细胞和HaCaT细胞的增殖;电压门控钾通道可能在人皮肤鳞状细胞癌细胞上的表达更显著.
目的 探討電壓門控鉀通道在人皮膚鱗狀細胞癌A431細胞和人角質形成細胞HaCaT細胞中的作用.方法 噻唑藍方法研究加入含有不同濃度四乙胺的培養基對A431細胞和HaCaT細胞的增殖的影響;提取體外培養A431細胞、HaCaT細胞以及人正常錶皮組織的蛋白,ELISA方法檢測電壓門控鉀通道蛋白(HERG)在它們中的錶達,比較它們的差異.結果 四乙胺對體外培養的A431細胞和HaCaT細胞的增殖抑製效應具有時間依賴與劑量依賴的特點,噹四乙胺的濃度≥10 mmol/L且作用時間≥24 h,可以使A431細胞和HaCaT細胞的增殖受到明顯的抑製.HERG鉀通道蛋白在A431細胞、HaCaT細胞和人正常皮膚錶皮組織均有錶達,平均濃度分彆為(49.7114±3.55696)pg/ml、(35.7471±4.14696)pg/ml、(36.8857±3.47810)pg/ml,組間比較差異具有統計學意義(P<0.05).結論 阻斷電壓門控鉀通道可抑製A431細胞和HaCaT細胞的增殖;電壓門控鉀通道可能在人皮膚鱗狀細胞癌細胞上的錶達更顯著.
목적 탐토전압문공갑통도재인피부린상세포암A431세포화인각질형성세포HaCaT세포중적작용.방법 새서람방법연구가입함유불동농도사을알적배양기대A431세포화HaCaT세포적증식적영향;제취체외배양A431세포、HaCaT세포이급인정상표피조직적단백,ELISA방법검측전압문공갑통도단백(HERG)재타문중적표체,비교타문적차이.결과 사을알대체외배양적A431세포화HaCaT세포적증식억제효응구유시간의뢰여제량의뢰적특점,당사을알적농도≥10 mmol/L차작용시간≥24 h,가이사A431세포화HaCaT세포적증식수도명현적억제.HERG갑통도단백재A431세포、HaCaT세포화인정상피부표피조직균유표체,평균농도분별위(49.7114±3.55696)pg/ml、(35.7471±4.14696)pg/ml、(36.8857±3.47810)pg/ml,조간비교차이구유통계학의의(P<0.05).결론 조단전압문공갑통도가억제A431세포화HaCaT세포적증식;전압문공갑통도가능재인피부린상세포암세포상적표체경현저.
Objective To investigate the role of voltage-gated potassium channel in the human skin squamous cell carcinoma A431 cells and human keratinocyte HaCaT cells. Methods MTT assay was performed to detect the effect of different concentrations of tetraethylammonium (TEA) on the proliferation of cultured A431 cells and HaCaT cells. Besides, enzyme-linked immunosorbent assay (ELISA) was conducted to detect the expression of HERG channel protein in A431 cells, HaCaT cells and normal human skin tissue.Results TEA inhibited the proliferation of A431 cells and HaCaT cells in a dose- and time-dependent manner.After treated with TEA (≥ 10 mmol/L) for 24 or more hours, the proliferation of A431 cells and HaCaT cells was obviously suppressed. A significant difference was observed in the average concentration of HERG channel protein between A431 cells, HaCaT cells and normal human skin tissue (49.7114 ± 3.55696 pg/ml, 35.7471 ±4.14696 pg/ml, 36.8857 ± 3.47810 pg/ml, all P < 0.05). Conclusions The block of voltage-gated potassium channel could inhibit the proliferation of A431 cells and HaCaT cells, and the expression of voltage-gated potassium channel seems to be higher in human skin squamous cell carcinoma cells.
