CAJ | 학술논문

目的观察凋亡基因Bcl/Bax与高脂饮食诱导大鼠的胰岛细胞凋亡的关系.方法应用TUNEL法检测高脂饮食诱导的大鼠胰岛细胞凋亡率,应用免疫组化方法检测bcl-2、bax的mRNA的表达.结果高脂饮食使大鼠胰岛细胞凋亡率明显增加.胰岛细胞凋亡过程中,诱导凋亡基因bax的mRNA表达水平明显升高,抵抗凋亡基因bcl-2 mRNA表达水平明显下降.结论高脂饮食可能通过诱导胰岛细胞凋亡导致或加重糖尿病,其中bcl-2/bax mRNA表达比率变化可能起重要作用.
목적 관찰조망기인Bcl/Bax여고지음식유도대서적이도세포조망적관계.방법 응용TUNEL법검측고지음식유도적대서이도세포조망솔,응용면역조화방법검측bcl-2、bax적mRNA적표체.결과 고지음식사대서이도세포조망솔명현증가.이도세포조망과정중,유도조망기인bax적mRNA표체수평명현승고,저항조망기인bcl-2 mRNA표체수평명현하강.결론 고지음식가능통과유도이도세포조망도치혹가중당뇨병,기중bcl-2/bax mRNA표체비솔변화가능기중요작용.
Objective To investigate the relation of apoptosis-related genes with apoptosis of rat pancreatic islet cells due to high fat diet. Methods Apoptotie cells were identified by TUNEL assay, mRNA levels of bcl-2 and Bax were evaluated by immunohistochemical staining. Results High level of fat diet increased the proportion of the apoptotic cell. In the process of apoptosis, mRNA level of Bax increased significantly, while bcl-2mRNA expression decreased. Conclusions High fat diet may induce and exacer-bate diabetes through inducing apoptosis of the islet cells. The change in the ratio of bel-2/Bax mRNA expression may play an important role in apoptosis.