CAJ | 학술논문

目的探讨子癎前期患者胎盘和血清中瘦素的表达变化及其和子癎前期发病的关系.方法采用免疫组化SP法检测45例子癎前期患者(研究组,其中重度组28例,轻度组17例)和30例同期正常妊娠妇女(对照组)胎盘瘦素水平,并用酶联免疫吸附实验检测两组孕妇产前血清瘦素水平.结果 (1)两组胎盘瘦素均在合体滋养细胞胞浆表达,随病情加重,染色逐渐加深,胎盘瘦素与子癎前期存在线性相关关系.(2)研究组、轻度组、重度组和对照组血清瘦素浓度分别为(10.41±4.78)ng/ml、(6.33±1.87)ng/ml、(12.88±4.27)ng/ml、(5.73±2.19)ng/ml,轻度组稍高于对照组但无统计学意义(P>0.05),研究组和对照组、轻和重度组、重度组和对照组相比,均有性统计学意义(P<0.01),瘦素表达水平和病情严重程度呈正相关.(3)血清瘦素与体重指数无相关性(P>0.05);但其与收缩压、舒张压及平均动脉压均呈正相关(r=0.602、0.566和0.585,P均<0.05).结论瘦素在子癎前期患者胎盘和血清中均高表达,高瘦素水平参与子癎前期的发生和发展.
목적 탐토자간전기환자태반화혈청중수소적표체변화급기화자간전기발병적관계.방법 채용면역조화SP법검측45례자간전기환자(연구조,기중중도조28례,경도조17례)화30례동기정상임신부녀(대조조)태반수소수평,병용매련면역흡부실험검측량조잉부산전혈청수소수평.결과 (1)량조태반수소균재합체자양세포포장표체,수병정가중,염색축점가심,태반수소여자간전기존재선성상관관계.(2)연구조、경도조、중도조화대조조혈청수소농도분별위(10.41±4.78)ng/ml、(6.33±1.87)ng/ml、(12.88±4.27)ng/ml、(5.73±2.19)ng/ml,경도조초고우대조조단무통계학의의(P>0.05),연구조화대조조、경화중도조、중도조화대조조상비,균유성통계학의의(P<0.01),수소표체수평화병정엄중정도정정상관.(3)혈청수소여체중지수무상관성(P>0.05);단기여수축압、서장압급평균동맥압균정정상관(r=0.602、0.566화0.585,P균<0.05).결론 수소재자간전기환자태반화혈청중균고표체,고수소수평삼여자간전기적발생화발전.
Objective To investigate the expression and alteration of leptin in placenta and serum in preeclampsia and its relationship with the disease. Methods Immunohistochemistry(SP) was used to determine the distribution and intensity of leptin staining in the placenta of 45 cases of preeclampsia(28 severe and 17 mild) and 30 cases of normal gravidas(contr01 group).Antepartum leptin level was examined using enzyme-linked immunosorbent assay method. Results (1)Leptin expression was located in the endochylema of the syncytiotrophoblast in both groups.Positive linear correlation existed between the leptin level and the severity of preeelampsia,expressed as darker staining with aggravating disease condition.(2)The serum leptin levels in all preeclampsia,mild and severe preeclampsia and normal group were(10.41±4.78)ng/ml,(6.33±1.87)ng/ml,(12.88±4.27)ng/ml and(5.73±2.19)ng/ml,respectively.Significant differences were found between preeclampsia women and normal group,mild and severe group,and severe and normal group(P<0.01).No significant difference was found when comparing mild and normal group(P>0.05).Positive linear correlation existed between the leptin expression and the severity of preeclampsia.(3)Serum leptin level had a positive correlation with systolic pressure,diastolic pressure,and mean arterial pressure(r=0.602,0.566 and 0.585,P<0.05),while no significant correlation was found between leptin level and patients'BMI(P>0.05).Conclusion Increased level of leptin in placenta and serum in preeclampsia may be involved in the occurrence and the development of the disease.