CAJ | 학술논문

目的：探讨MDM2基因在鼻咽癌(NPC)中的表达情况及其与p53蛋白表达、EB病毒(EBV)潜伏感染的关系。方法：运用非同位素原位杂交、免疫组化和多聚酶链反应技术对46例NPC、12例慢性鼻咽炎症粘膜(CINE)分别进行MDM2 mRNA、MDM2蛋白、p53蛋白及EBV-DNA的检测。结果：46例NPC中，14例为MDM2 mRNA及MDM2蛋白高表达，38例为p53蛋白阳性，43例为EBV-DNA阳性；12例CINE均无MDM2mRNA、MDM2蛋白及p53蛋白表达；MDM2的高表达与p53蛋白的表达相关(P<0．05)，而与EBV-DNA无关。结论：MDM2可能通过与p53蛋白相互作用而在NPC的发生过程中起着重要作用。
목적：탐토MDM2기인재비인암(NPC)중적표체정황급기여p53단백표체、EB병독(EBV)잠복감염적관계。방법：운용비동위소원위잡교、면역조화화다취매련반응기술대46례NPC、12례만성비인염증점막(CINE)분별진행MDM2 mRNA、MDM2단백、p53단백급EBV-DNA적검측。결과：46례NPC중，14례위MDM2 mRNA급MDM2단백고표체，38례위p53단백양성，43례위EBV-DNA양성；12례CINE균무MDM2mRNA、MDM2단백급p53단백표체；MDM2적고표체여p53단백적표체상관(P<0．05)，이여EBV-DNA무관。결론：MDM2가능통과여p53단백상호작용이재NPC적발생과정중기착중요작용。
Objective:To define the expression levels of MDM2 gene in nasopharyngeal carcinoma (NPC)and its relationship with p53 protein expression and EB virus latent infection. Method :MDM2 gene expression atmRNA and protein levels,p53 protein and EB virus DNA were detected by nonradioactive in situ hybridization(ISH) ,immunohistochemistry(IHC) and polymerase chain reaction (PCR) separately in 46 cases of NPC tissuesand 12 cases of chronic inflammation of nasopharyngeal epithelium (CINE). Result: Fourteen cases of NPCshowed MDM2 mRNA and protein overexpression, 38 cases were p53 protein positive,and 43 cases were EBV-DNA positive. Neither MDM2 nor p53 protein was expressed in any case of CINE. MDM2 expression was signifi-cantly related to p53 protein expression ( P ＜0. 05), but not to EB virus latent infection in NPC. Conclusion:MDM2 gene may play an important role in the pathogenesis of NPC through interacting with p53 protein.