华中农业大学学报
華中農業大學學報
화중농업대학학보
JOURNAL OF HUAZHONG AGRICULTURAL UNIVERSITY
2009年
6期
645-650
,共6页
董华林%张晨昕%曾波%孙文强%余四斌
董華林%張晨昕%曾波%孫文彊%餘四斌
동화림%장신흔%증파%손문강%여사빈
普通野生稻%高世代回交群体%数量性状位点
普通野生稻%高世代迴交群體%數量性狀位點
보통야생도%고세대회교군체%수량성상위점
common wild rice%advanced backcross population%quantitative trait loci(QTLs)
本研究用来源于马来西亚的普通野生稻(IRGC-105491)与珍汕97B杂交并回交构建148个株系的高代回交群体(BC_2F_4),用152个均匀分布的SSR标记构建了分子遗传连锁图,其图谱长为1 342.1 cM,相邻标记间距为8.8 cM.利用该群体检测定位到影响株高、生育期、穗数、穗长及千粒重、粒长、粒宽等农艺性状的27个QTL;在这些QTL中约有59%有利基因来源于野生稻.野生稻中有利基因的发掘和利用将为分子标记辅助培育水稻新品种奠定基础.
本研究用來源于馬來西亞的普通野生稻(IRGC-105491)與珍汕97B雜交併迴交構建148箇株繫的高代迴交群體(BC_2F_4),用152箇均勻分佈的SSR標記構建瞭分子遺傳連鎖圖,其圖譜長為1 342.1 cM,相鄰標記間距為8.8 cM.利用該群體檢測定位到影響株高、生育期、穗數、穗長及韆粒重、粒長、粒寬等農藝性狀的27箇QTL;在這些QTL中約有59%有利基因來源于野生稻.野生稻中有利基因的髮掘和利用將為分子標記輔助培育水稻新品種奠定基礎.
본연구용래원우마래서아적보통야생도(IRGC-105491)여진산97B잡교병회교구건148개주계적고대회교군체(BC_2F_4),용152개균균분포적SSR표기구건료분자유전련쇄도,기도보장위1 342.1 cM,상린표기간거위8.8 cM.이용해군체검측정위도영향주고、생육기、수수、수장급천립중、립장、립관등농예성상적27개QTL;재저사QTL중약유59%유리기인래원우야생도.야생도중유리기인적발굴화이용장위분자표기보조배육수도신품충전정기출.
An advanced backcross(AB) population(BC_2F_4) was developed by successive backcrossing between the common wild rice(IRGC-105491) from Malaysia as donor parent, and the elite Chinese variety Zhenshan97B as recurrent parent. A genetic linkage map with 152 SSR markers covering the genome of 1 342. 1 cM,with an average interval of 8. 8 cM was constructed for QTL mapping using the AB population. One segregation distortion hot spot is found on chromosome 1. Twenty-seven QTLs are identified for 8 agronomic traits such as plant height, heading date, panicle number, panicle length, 1000-grain weight, grain length and width, among them about 59 % favorable alleles are from the wild rice. The results will provide a good foundation for molecular assisted transferring the desirable alleles from the wild relatives in rice breeding.