CAJ | 학술논문

以PolyI:C(又称聚肌胞)为诱导剂,分别通过腹腔注射、浸泡和投喂3种途径诱导大菱鲆 Scophthalmus maximus体内抗病毒蛋白Mx基因的转录,利用半定量RT-PCR方法检测干扰素下游基因-Mx基因的转录水平来确定该诱导剂的诱导效果.结果显示,以上3种途径都能高效诱导Mx蛋白mRNA的转录,均在48h之后达到高峰,其中以浸泡的方式更容易诱导Mx基因转录,且在120h时仍保持较高水平.Mx基因转录的时相变化证明了国产PolyI:C可以通过多种途径诱导抗病毒蛋白Mx的表达,为实际应用中确定用药途径提供了理论依据.另外,实验初步建立了半定量RT-PCR方法,为检测鱼体内干扰素的表达提供了技术方法.
이PolyI:C(우칭취기포)위유도제,분별통과복강주사、침포화투위3충도경유도대릉평 Scophthalmus maximus체내항병독단백Mx기인적전록,이용반정량RT-PCR방법검측간우소하유기인-Mx기인적전록수평래학정해유도제적유도효과.결과현시,이상3충도경도능고효유도Mx단백mRNA적전록,균재48h지후체도고봉,기중이침포적방식경용역유도Mx기인전록,차재120h시잉보지교고수평.Mx기인전록적시상변화증명료국산PolyI:C가이통과다충도경유도항병독단백Mx적표체,위실제응용중학정용약도경제공료이론의거.령외,실험초보건립료반정량RT-PCR방법,위검측어체내간우소적표체제공료기술방법.
Transcription of turbot Mx protein confirmed to be an antivirus factor was induced by PolyI:C with intraperitoneal injection, immersion and oral administration. Mx mRNA was tested by semi-quantitative RT-PCR to validate the effect of PolyI:C's induction. The results illustrated that all the three induction ways could effectively induce the transcription of Mx gene and the peak of Mx mRNA quantity appeared at 48h after PolyI:C application. The immersion method seemed to be the most effective way to induce Mx mRNA, as Mx mRNA could last more than 120h at a high level. This experiment proved that the homemade PolyI:C could effectively induce Mx mRNA transcription in different applications, which provided a reference to use PolyI:C in aquaculture practice.