CAJ | 학술논문

[目的]探讨白细胞介素8基因(interleukin-8,IL-8)基因A-251T和载脂蛋白E(ApoE)基因多态性与迟发性阿尔茨海默病(LOAD)的相关性.[方法]采用聚合酶链反应-限制性片段长度多态性方法(PCR-RFLP)观察185例广东汉族人群(研究组:LOAD患者88例,正常对照组97例)IL-8基因A-251T基因、ApoE基因多态性的分布并进行关联分析.[结果]①IL-8基因A-251T各基因型及等位基因在研究组及对照组间的差异均无统计学意义.②ApoE e4等位基因频率在研究组中高于对照组,差异有统计学意义,为LOAD的患病危险因素(OR=2.272,P=0.003).③按是否携带ApoE e4等位基因将研究组和对照组均分为携带ε4组与非携带ε4组.无论是否携带ApoE ε4等位基因.IL-8基因A-251T各基因型频率在研究组和对照组间无统计学差异(携带ε4组:P=0.965;非携带ε4组:P=0.523).[结论]IL-8基因A-251T多态可能与LOAD无关,与ApoE ε4可能不存在相互作用.
[목적]탐토백세포개소8기인(interleukin-8,IL-8)기인A-251T화재지단백E(ApoE)기인다태성여지발성아이자해묵병(LOAD)적상관성.[방법]채용취합매련반응-한제성편단장도다태성방법(PCR-RFLP)관찰185례엄동한족인군(연구조:LOAD환자88례,정상대조조97례)IL-8기인A-251T기인、ApoE기인다태성적분포병진행관련분석.[결과]①IL-8기인A-251T각기인형급등위기인재연구조급대조조간적차이균무통계학의의.②ApoE e4등위기인빈솔재연구조중고우대조조,차이유통계학의의,위LOAD적환병위험인소(OR=2.272,P=0.003).③안시부휴대ApoE e4등위기인장연구조화대조조균분위휴대ε4조여비휴대ε4조.무론시부휴대ApoE ε4등위기인.IL-8기인A-251T각기인형빈솔재연구조화대조조간무통계학차이(휴대ε4조:P=0.965;비휴대ε4조:P=0.523).[결론]IL-8기인A-251T다태가능여LOAD무관,여ApoE ε4가능불존재상호작용.
[Objective] To investigate the relation among the polymorphism of interleukin-8 (IL-8) gene A-251T and apolipoprotein E (ApoE) gene with late-onset Alzheimer's disease (LOAD). [Methods] Polymerase chain reaction and restrietive fragment length polymorphism (PCR-RFLP) were used to detect the polymorphism distribution of IL-8 gene A-251T and ApoE gene of 185 people in Han ethnicity of Guangdong province (study group: 88 LOAD patients, eontrolled group: 97 people). The correlation of these genes was analyzed. [Results] (1) The differences of genotypes and alleles of IL-8 gene A-251T between study group and controlled group had no statistical significance. (2) The frequency of ApoE 64 allele in study group was significantly higher than that in controlled group, as risk factor of LOAD (OR=2.272, P=0.003). (3) Study group and controlled group were both divided into two groups by carrying ApoE ε4 allele or not. The differenees of IL-8 genotypes were no significant whether in carrying ApoE ε4 allele group or not (carrying ε4 group: P=0.965; no carrying ε4 group: P=0.523). [Conclusion] It is suggested that lL-8 gene A-251T polymorphism perhaps has no relation with LOAD and has no interaction with ApoE ε4 allele.