CAJ | 학술논문

目的探讨胚胎期和哺乳期不同程度的铅暴露对断乳仔鼠海马组织代谢型谷氨酸受体5亚型(mGluR5)mRNA和蛋白表达的影响.方法健康成年雌性SD大鼠分为高铅组、中铅组、低铅组和对照组,分别给予0.5%、0.2%、0.05%醋酸铅溶液和双蒸水自由饮水,1周后雌雄合笼,仔鼠断乳时用原子吸收和等离子电感耦合质谱仪测定仔鼠血铅和海马铅含量,用荧光定量PCR和Western blot检测发育脑海马mGluR5 mRNA和蛋白的表达水平.结果铅暴露组血铅和海马铅含量高于对照组,且铅暴露剂量越大,血铅和海马铅含景越高(P<0.05).高铅组、中铅组和低铅组mGiuR5 mRNA表达量与正常对照组相比下降,比值分别为0.66、0.78和0.82.高铅组、中铅组和低铅组mGluR5蛋白表达较正常对照组下调,铅暴露剂最越高,mGluR5蛋白表达水平越低,差异有统计学意义(P<0.05).结论海马mGluR5可能参与了铅对神经发育的毒性作用,可能是铅神经发育毒性的中间环节之一.
목적 탐토배태기화포유기불동정도적연폭로대단유자서해마조직대사형곡안산수체5아형(mGluR5)mRNA화단백표체적영향.방법 건강성년자성SD대서분위고연조、중연조、저연조화대조조,분별급여0.5%、0.2%、0.05%작산연용액화쌍증수자유음수,1주후자웅합롱,자서단유시용원자흡수화등리자전감우합질보의측정자서혈연화해마연함량,용형광정량PCR화Western blot검측발육뇌해마mGluR5 mRNA화단백적표체수평.결과연폭로조혈연화해마연함량고우대조조,차연폭로제량월대,혈연화해마연함경월고(P<0.05).고연조、중연조화저연조mGiuR5 mRNA표체량여정상대조조상비하강,비치분별위0.66、0.78화0.82.고연조、중연조화저연조mGluR5단백표체교정상대조조하조,연폭로제최월고,mGluR5단백표체수평월저,차이유통계학의의(P<0.05).결론해마mGluR5가능삼여료연대신경발육적독성작용,가능시연신경발육독성적중간배절지일.
Objective The effect of lead exposure on children is consistently associated with intellectual and other neurologic deficits.However the exact mechanism by which Pb~(2+) exerts toxic effects on developmental central nervous system remains unknown.Our group has found by gene-chip test that the expression of metabotropic glutamate receptor subtype 5 (mGluR5) mRNA was changed by lead exposure.The present study aimed to examine the effects of different level of lead exposure on the expression of mCluR5 in gestation and lactation.Methods Sprague-Dawley rats were exposed to lead acetate during gestation and lactation.Three concentrations of 0.05%,0.2%,and 0.5% lead acetate were applied and considered as low,middle and high exposure group respectively.The Pb levels of blood and hippocampus of pups were analyzed at weaning to evaluate the actual lead content at the end of the exposure.The impact of lead exposure on the expression of mGluR5 mRNA and protein in hippocampal tissue of pups was investigated by quantitative real-time polymerase chain reaction (PCR) and Western blot.The potential role of the expression of mGluR5 mRNA and protein in lead neurotoxicity were discussed.Results The levels of lead in blood and hippocampus from lead-exposed rats were significantly higher than those in the controls and positively related to the degree of lead exposure.The results of real-time PCR and Western blot showed that exposure to lead acetate decreased the expression of mCluR5 mRNA and protein with a dose-dependent manner.Conclusions Hippocampal mGluR5 might be involved in lead-induced neurotoxicity.