CAJ | 학술논문

背景:为实现纳米基因药物在治疗肿瘤中实现高靶向性,课题采用聚乙二醇和聚乙烯亚胺修饰的磁性四氧化三铁纳米粒(PEG-PEI/Fe_3O_4)作为基因药物载体,在提高载体载药能力的同时,使载体具有超顺磁性,增加药物靶向性能.目的:评价PEG-PEI/Fe_3O_4纳米磁流体体外和体内毒性.方法:对制备的PEG-PEI/_3O_4纳米磁流体进行表征达到纳米材料水平后,过滤稀释5～20倍培养7702细胞和HpG_2细胞,进行体外MTT毒性试验;通过体内溶血试验和微核试验测定生物相容性和体内毒性.结果与结论:MTT结果显示,PEG-PEI/Fe_3O_4纳米磁流体对7702细胞毒性0～1级,对正常肝细胞无害;PEG-PEI/Fe_3O_4纳米磁流体对HpG_2细胞有轻微的旁观者抑制效应;PEG-PEI/Fe_3O_4纳米磁流体溶血率为0.372%,远小于5%;微核试验结果表明PEG-PEI/Fe_3O_4纳米磁流体无致畸、致突变作用.
배경:위실현납미기인약물재치료종류중실현고파향성,과제채용취을이순화취을희아알수식적자성사양화삼철납미립(PEG-PEI/Fe_3O_4)작위기인약물재체,재제고재체재약능력적동시,사재체구유초순자성,증가약물파향성능.목적:평개PEG-PEI/Fe_3O_4납미자류체체외화체내독성.방법:대제비적PEG-PEI/_3O_4납미자류체진행표정체도납미재료수평후,과려희석5～20배배양7702세포화HpG_2세포,진행체외MTT독성시험;통과체내용혈시험화미핵시험측정생물상용성화체내독성.결과여결론:MTT결과현시,PEG-PEI/Fe_3O_4납미자류체대7702세포독성0～1급,대정상간세포무해;PEG-PEI/Fe_3O_4납미자류체대HpG_2세포유경미적방관자억제효응;PEG-PEI/Fe_3O_4납미자류체용혈솔위0.372%,원소우5%;미핵시험결과표명PEG-PEI/Fe_3O_4납미자류체무치기、치돌변작용.
BACKGROUND: Polyethylene glycol-polyethyleneimine/ferroso-ferric oxide (PEG-PEI/Fe_3O_4) was selected as drug carders in tumor treatment, which can increase drug loading capacity and targeting capacity.OBJECTIVE: To test the toxicity of PEG-PEI/Fe_3O_4 nano-magnetic fluid in vitro and in vivo. METHODS: When the prepared PEG-PEI/Fe_3O_4 nano-magnetic fluid reached nano level, 7702 and HpG2 cell lines were filtrated and diluted in 5-20 multiple, and detected by in vitro MTT toxicity test assay; in vivo hemolysis test and micronucleus test was used to test the toxicity and biocompatibility.RESULTS AND CONCLUSION: MTT assay results indicated that the toxicity grade of PEG-PEI/Fe_3O_4 nano-magnetic fluid to 7702 cell line was 0-1, which was harmless to natural hepatic cells; however, PEG-PEI/Fe304 nano-magnetic fluid had slight bystander restraining effect to HpG_2 cell line. Maximum hemolysis rate of the matedel was 0.372%, which was far less than 5%. The micronucieus test result indicated that PEG-PEI/Fe_3O_4 nano-magnetic fluid had no teratogenicity or mutagenicity.