CAJ | 학술논문

目的建立测定左旋尤利沙星含量及有关物质的高效液相色谱方法.方法采用Diamonsil C_(18)柱(250mm×4.6mm,5μm),以乙腈-0.2%三乙胺和0.1%庚烷磺酸钠水溶液(用磷酸调节pH 2.5)(25:75)为流动相,流速为1.0ml/min,检测波长为275nm,柱温为35℃.结果主峰能与相邻杂质峰达基线分离,左旋尤利沙星的浓度在10～200μg/ml范围内线性关系良好,相关系数为0.9996,最低检测限为0.2ng.结论该法简便、准确,灵敏度高,专属性强,可以用于左旋尤利沙星的含量及有关物质的测定.
목적 건립측정좌선우리사성함량급유관물질적고효액상색보방법.방법 채용Diamonsil C_(18)주(250mm×4.6mm,5μm),이을정-0.2%삼을알화0.1%경완광산납수용액(용린산조절pH 2.5)(25:75)위류동상,류속위1.0ml/min,검측파장위275nm,주온위35℃.결과 주봉능여상린잡질봉체기선분리,좌선우리사성적농도재10～200μg/ml범위내선성관계량호,상관계수위0.9996,최저검측한위0.2ng.결론 해법간편、준학,령민도고,전속성강,가이용우좌선우리사성적함량급유관물질적측정.
Objective To develop an HPLC method for determination of levoulifloxacin and its related substances.Methods The chromatographic separation of levoulifloxacin was performed on a Diamonsil C_(18) column with a mobile phase consisting of acetonitrile-0.2%triethylamine and 0.1%sodium heptanesulfonic acid at pH 2.5 the column temperature was maintained at 35℃.Results Levoulifloxacin was completely separated from its related substances.The calibration curve of levoulifloxacin was linear in the range of 10-200 μg/ml with the correlation coefficient of 0.9996.The limit of detection was 0.2ng.Conclusions The method is simple,rapid,sensitive and specific,and can be used for determination of levoulifloxacin and its related substances.