CAJ | 학술논문

实时定量PCR(Real-time Quantitative PCR,RQ-PCR)凭其特异性强、定量准确和速度快等优点已经成为快速检测和研究植物真茵病害的重要工具.文章就这项技术在植物真茵病原茵定性和定量检测、土壤栖息的植物真菌病原体和寄主组织中真茵DNA的定量检测等方面的应用研究进行了综述.大量研究表明,快速发展的实时定量PCR技术正在开创一个植物真菌病害的诊断学、传染病学和寄主-病原体相互作用研究的新领域.
실시정량PCR(Real-time Quantitative PCR,RQ-PCR)빙기특이성강、정량준학화속도쾌등우점이경성위쾌속검측화연구식물진인병해적중요공구.문장취저항기술재식물진인병원인정성화정량검측、토양서식적식물진균병원체화기주조직중진인DNA적정량검측등방면적응용연구진행료종술.대량연구표명,쾌속발전적실시정량PCR기술정재개창일개식물진균병해적진단학、전염병학화기주-병원체상호작용연구적신영역.
Real-time Quantitative PCR(RQ-PCR)has become an important tool for rapidly detecting and studying fungal plant disease depending on its high specificity,exact quantitation and fast speed.The article reviewed this technology application in detection of fungal plant disease,including pathogenic bactedum qualitative and quantitative detection of fungal plant disease,fungal DNA quantification in host tissue and soil-inhabiting fungi.These studies showed that rapid growth RQ-PCR was opening a new research opportunity for the study of diagnosis,epidemiology and host-pathogen interactions.