CAJ | 학술논문

背景:FGL是NCAM的核心活性多肽片段,可直接作用于成纤维细胞生长因子受体1,激活NCAM的信号传导途径.目的:观察FGL人工合成多肽联合培养对PC12细胞增殖和凋亡的作用.方法:将培养的PC12细胞分为对照组和实验组,实验组预先加入1%的FGL多肽溶液.分别于培养1,3,5,7,9 d采用细胞计数试剂8法检测细胞增殖情况.将PC12细胞分为正常组、实验组和损伤组,损伤组加入H2O2刺激16 h.实验组加入H2O2与FGL人工合成多肽刺激16 h,流式细胞仪检测细胞凋亡,荧光定量PCR法检测PC12中的核转录因子κB mRNA表达.结果与结论:FGL人工合成多肽与PC12复合培养细胞生长良好,可明显促进PC12细胞的活性并且减低PC12 细胞凋亡并可明显降低凋亡模型中PC12细胞核转录因子κB基因的表达.说明FGL多肽可以明显促进PC12细胞增殖,并可以抑制PC12细胞凋亡.
배경:FGL시NCAM적핵심활성다태편단,가직접작용우성섬유세포생장인자수체1,격활NCAM적신호전도도경.목적:관찰FGL인공합성다태연합배양대PC12세포증식화조망적작용.방법:장배양적PC12세포분위대조조화실험조,실험조예선가입1%적FGL다태용액.분별우배양1,3,5,7,9 d채용세포계수시제8법검측세포증식정황.장PC12세포분위정상조、실험조화손상조,손상조가입H2O2자격16 h.실험조가입H2O2여FGL인공합성다태자격16 h,류식세포의검측세포조망,형광정량PCR법검측PC12중적핵전록인자κB mRNA표체.결과여결론:FGL인공합성다태여PC12복합배양세포생장량호,가명현촉진PC12세포적활성병차감저PC12 세포조망병가명현강저조망모형중PC12세포핵전록인자κB기인적표체.설명FGL다태가이명현촉진PC12세포증식,병가이억제PC12세포조망.
BACKGROUND: FG loop (FGL) is a core active peptide fragment of neural cell adhesion molecule (NCAM), which can directly act on fibroblast growth factor receptor 1 (FGFR1) to activate NCAM signal pathway.OBJECTIVE: To observe the effects of synthetic peptides FGL on PC12 cells proliferation and apoptosis.METHODS: ①PC12 cells proliferation and apoptosis: The cultured PC12 cells were divided into control group and experiment group. The experimental group was added with 1% FGL peptide solution. The control group was pre-coated with poly-lysine plates. The cells were cultured 1, 3, 5, 7, 9 d respectively to detect cell proliferation by using Cell Counting Kit-8. ②PC12 apoptosis and nuclear factor kappa B mRNA detection: The PC12 cells were divided into normal group, experimental group and injury group. H2O2 was added into the injury group for 16 hours stimulation. In the experimental group, H2O2 and FGL were used for 16 hours stimulation. The cell apoptosis were detected by flow cytometry; mRNA expression of nuclear factor kappa B was detected by quantitative fluorescent polymerase chain reaction.RESULTS AND CONCLUSION: PC12 cells cocultured with FGL peptide grow well, which indicates that FGL peptides can promote PC12 cell proliferation and inhibit PC12 cell apoptosis, as well as decrease mRNA expression of nuclear factor kappa B.