CAJ | 학술논문

目的探讨脂联素在子痫前期患者胎盘组织中的表达与其发病的关系.方法采用免疫组化链霉菌抗生物蛋白-过氧化物连接(SP)法及RT-PCR技术,检测20例正常足月妊娠孕妇(正常妊娠组)、12例轻度子痫前期(轻度子痫前期组)及22例重度子痫前期(重度子痫前期组)患者胎盘组织中脂联素蛋白及其mRNA的表达,并分析其与子痫前期发病的关系.结果 (1)3组孕妇胎盘绒毛合体滋养细胞及细胞滋养细胞胞质内脂联素蛋白均呈阳性表达,且各组内胎盘母面及子面脂联素蛋白的表达水平相互比较,差异均无统计学意义(P＞0.05).(2)重度子痫前期组胎盘组织中脂联素蛋白的表达水平(30 984±14 604)低于轻度子痫前期组(58 360±8910)及正常妊娠组(53 246±17 554),差异均有统计学意义(P＜0.01).重度子痫前期组中妊娠足月者胎盘组织中脂联素蛋白的表达水平(38 890±20 386)与未足月者(29 319±8997)比较,差异无统计学意义(P＞0.05);但与正常妊娠组比较,差异有统计学意义(P＜0.05).(3)3组孕妇胎盘组织中均有脂联素mRNA的表达.其中重度子痫前期组胎盘组织中脂联素mRNA表达水平(1.0±0.2)低于轻度子痫前期组(2.9±0.8)及正常妊娠组(3.3±1.1),差异有统计学意义(P＜0.05).结论重度子痫前期患者胎盘组织中脂联素mRNA表达水平下降导致其蛋白表达水平也下降,提示脂联素的异常表达参与了子痫前期的发病.
목적 탐토지련소재자간전기환자태반조직중적표체여기발병적관계.방법 채용면역조화련매균항생물단백-과양화물련접(SP)법급RT-PCR기술,검측20례정상족월임신잉부(정상임신조)、12례경도자간전기(경도자간전기조)급22례중도자간전기(중도자간전기조)환자태반조직중지련소단백급기mRNA적표체,병분석기여자간전기발병적관계.결과 (1)3조잉부태반융모합체자양세포급세포자양세포포질내지련소단백균정양성표체,차각조내태반모면급자면지련소단백적표체수평상호비교,차이균무통계학의의(P＞0.05).(2)중도자간전기조태반조직중지련소단백적표체수평(30 984±14 604)저우경도자간전기조(58 360±8910)급정상임신조(53 246±17 554),차이균유통계학의의(P＜0.01).중도자간전기조중임신족월자태반조직중지련소단백적표체수평(38 890±20 386)여미족월자(29 319±8997)비교,차이무통계학의의(P＞0.05);단여정상임신조비교,차이유통계학의의(P＜0.05).(3)3조잉부태반조직중균유지련소mRNA적표체.기중중도자간전기조태반조직중지련소mRNA표체수평(1.0±0.2)저우경도자간전기조(2.9±0.8)급정상임신조(3.3±1.1),차이유통계학의의(P＜0.05).결론 중도자간전기환자태반조직중지련소mRNA표체수평하강도치기단백표체수평야하강,제시지련소적이상표체삼여료자간전기적발병.
Objective To investigate the expression of adiponectin in placenta and its correlation with preeclampsia.Methods Placental tissues were collected from normal term pregnancies(normal pregnancy group,n=20),mild preeclampsia(mild preeclampsia group,n=12)and severe preeclampsia (severe preeclampsia group,n=22).The expression of adiponectin protein and the intensity of its mRNA in placenta were detected using immunohistochemistry and RT-PCR,respectively.Integral optical density (IOD)which represents the expression level of adiponectin protein,and the ratio of adiponectin cDNA PCR products to β-actin cDNA PCR products which represents the intensity of transcription of adiponectin mRNA in placenta were analyzed.Results (1)The expression of adiponectin protein was observed in cytoplasm of placental cytotrophoblasts and syncytiotrophoblasts among three groups.There was no significant difference in adiponectin protein expression between maternal side and fetal side of placenta in three groups(all P＞0.05);(2)The expression of adiponectin protein in placenta in severe preeclampsia group(30 984 ±14 604)was significantly lower than that of mild preeclampsia group(58 360±8910,P＜0.01)and of normal pregnancy group(53 246±17 554,P＜0.01).There was also no significant difference in the expression of adiponeclln protein in placenta between term delivery and preterm delivery in severe preeclampsia group(38 890±20 386 vs 29 319±8997,P＞0.05),however,the expression of adiponectin protein in placenta in term delivery of severe preeclampsia group was significantly lower than that ofterm delivery of normlal pregnancy group(38 890±20 386 vs 53 246±17 554,P＜0.05);(3)The expression of adiponectin mRNA was detected in placental tissues among three groups also.The intensity of transcription of adiponectin in placenta in severe preeclampsia group(1.0±0.2)was markedly lower than that of mild preeclampsia group(2.9±0.8,P＜0.05)and normal pregnancy group(3.3±1.1,P=0.000).Conclusion The expression of adiponectin decreases in placenta tissues of severe preeclampsia,indicating that the abnormal expression of adiponectin may be involved in the pathogenesis of preeclampsia.