CAJ | 학술논문

目的观察携带人Endostatin基因的重组腺病毒载体Ad-hEnd对裸鼠胰腺癌移植瘤的治疗作用.方法将胰腺癌细胞株SW1990细胞皮下注射建立裸鼠移植瘤模型,随机分为Ad-hEnd组、报告基因LacZ重组腺病毒组(Ad-LacZ组)和对照组,每组8只.重组腺病毒200 μl瘤内注射,隔日1次,共4次.观察移植瘤的生长情况,免疫组化染色检测血管内皮生长因子(VEGF)的表达和微血管密度(MVD),原位缺口末端标记法(TUNEL)检测肿瘤细胞凋亡.结果移植瘤成瘤率100%.治疗后4周,Ad-hEnd组、Ad-LacZ组和对照组移植瘤体积分别为(921.9±279.7)mm3、(2804.4±553.5)mm3和(3040.6±487.6)mm3;瘤重分别为(1.19±0.18)g、(2.38±0.42)g和(2.41±0.47)g;VEGF表达阳性率分别为(36.3±7.1)%、(81.2±6.6)%和(79.4±6.2)%;MVD分别为12±4、27±5和25±6;细胞凋亡率分别为(31.2±5.4)%、(9.4±4.9)%和(8.5±3.7)%.与Ad-LacZ组和对照组比较,Ad-hEnd组以上各项指标均有显著性差异(P＜0.01);Ad-LacZ组和对照组之间的差异无统计学意义.结论重组腺病毒介导的hEndostatin基因可抑制胰腺癌的生长和血管生成,促进肿瘤细胞凋亡,可用于胰腺癌抗血管生成的基因治疗.
목적 관찰휴대인Endostatin기인적중조선병독재체Ad-hEnd대라서이선암이식류적치료작용.방법 장이선암세포주SW1990세포피하주사건립라서이식류모형,수궤분위Ad-hEnd조、보고기인LacZ중조선병독조(Ad-LacZ조)화대조조,매조8지.중조선병독200 μl류내주사,격일1차,공4차.관찰이식류적생장정황,면역조화염색검측혈관내피생장인자(VEGF)적표체화미혈관밀도(MVD),원위결구말단표기법(TUNEL)검측종류세포조망.결과 이식류성류솔100%.치료후4주,Ad-hEnd조、Ad-LacZ조화대조조이식류체적분별위(921.9±279.7)mm3、(2804.4±553.5)mm3화(3040.6±487.6)mm3;류중분별위(1.19±0.18)g、(2.38±0.42)g화(2.41±0.47)g;VEGF표체양성솔분별위(36.3±7.1)%、(81.2±6.6)%화(79.4±6.2)%;MVD분별위12±4、27±5화25±6;세포조망솔분별위(31.2±5.4)%、(9.4±4.9)%화(8.5±3.7)%.여Ad-LacZ조화대조조비교,Ad-hEnd조이상각항지표균유현저성차이(P＜0.01);Ad-LacZ조화대조조지간적차이무통계학의의.결론 중조선병독개도적hEndostatin기인가억제이선암적생장화혈관생성,촉진종류세포조망,가용우이선암항혈관생성적기인치료.
Objective To construct a human endostatin adenovirus vector and investigate its inhibitory effect on pancreatic carcinoma in nude mice.Methods Animal model of pancreatic carcinoma bearing nude mice was established by subcutaneous injection of SW1990 cells.All mice were randomized into Ad-hEnd group,Ad-LacZ group and control group with 8 mice in each group.The endostatin gene recombinant adenovirus were intratumorally injected every two days for 4 times.The rate of tumor growth was observed.lmmunohistochemical staining was employed to investigate the expression of vascular endothelial growth factor (VEGF) and micro-vessel density (MVD).TUNEL in situ was used to examine tumor cell apoptosis.Results The tumor formation rate was 100%.4 weeks later,the volumes of the tumors were (921.9±279.7 )mm3,(2804.4±553.5 )mm3 and ( 3040.6±487.6 ) mm3 in Ad-hEnd group,Ad-LacZ group and control group,respectively;the weights of the tumors were (1.19±0.18 ) g,( 2.38±0.42 ) g and ( 2.41±0.47 ) g,respectively;the VEGF positive rates were (36.3±7.1 )%,(81.2±6.6)% and (79.4±6.2)%,respectively;the levels of MVD were 12±4,27±5 and 25±6,respectively;the apoptotic rates were (31.2 ±5.4) %,( 9.4±4.9 ) % and ( 8.5±3.7 ) %,respectively.Compared with Ad-LacZ group and control group,the parameters in Ad-hEnd group were statistically different (P ＜0.01 ).The difference betweon Ad-LacZ group and control group was not statistically different.Conclusions Human endostatin gene mediated by recombinant adenovirus could inhibit tumor growth,angiogenesis and promote cell apoptosis of pancreatic carcinoma and could be used as geue therapy for pancreatic carcinoma.