CAJ | 학술논문

目的探讨脊髓损伤后神经干细胞(neural stem cells,NSCs)移植对大鼠损伤脊髓病理和超微结构的影响,以及免疫抑制剂他克莫司( FK506)对神经再生的保护作用. 方法采用成年SD大鼠,制作T8节段脊髓压迫损伤模型.动物按随机数字表法分为对照组、FK506组、NSCs组和NSCs+FK506组.伤后7,14,28,56 d应用运动诱发电位(motor - evoked potential,MEP)监测、HE染色、免疫组织化学染色、超微结构观察及有髓纤维图像分析比较各组神经再生差异. 结果伤后28 d NSCs+ FK506组MEP潜伏期与其他各组比较明显缩短(P＜0.05).HE染色显示,56 d时NSCs+ FK506组只出现灶性坏死.与对照组比较,BrdU和NF - 200免疫组织化学染色示各组阳性细胞数增多,且NSCs+ FK506组阳性细胞数高于其他两组.电镜扫描结果显示,56 d时对照组仍可见大的神经髓鞘轴膜下水肿,NSCs+ FK506组可见典型的新生髓鞘和轴索,神经纤维再生效果均比其他各组好(P＜0.01). 结论脊髓损伤大鼠移植NSCs后,早期联合应用FK506可改善神经再生纤维的病理和超微结构,具有良好的促神经再生作用.
목적 탐토척수손상후신경간세포(neural stem cells,NSCs)이식대대서손상척수병리화초미결구적영향,이급면역억제제타극막사( FK506)대신경재생적보호작용. 방법 채용성년SD대서,제작T8절단척수압박손상모형.동물안수궤수자표법분위대조조、FK506조、NSCs조화NSCs+FK506조.상후7,14,28,56 d응용운동유발전위(motor - evoked potential,MEP)감측、HE염색、면역조직화학염색、초미결구관찰급유수섬유도상분석비교각조신경재생차이. 결과 상후28 d NSCs+ FK506조MEP잠복기여기타각조비교명현축단(P＜0.05).HE염색현시,56 d시NSCs+ FK506조지출현조성배사.여대조조비교,BrdU화NF - 200면역조직화학염색시각조양성세포수증다,차NSCs+ FK506조양성세포수고우기타량조.전경소묘결과현시,56 d시대조조잉가견대적신경수초축막하수종,NSCs+ FK506조가견전형적신생수초화축색,신경섬유재생효과균비기타각조호(P＜0.01). 결론 척수손상대서이식NSCs후,조기연합응용FK506가개선신경재생섬유적병리화초미결구,구유량호적촉신경재생작용.
Objective To investigate the impacts of neural stem cells (NSCs) transplantation on spinal pathology and ultrastructure after spinal cord injury (SCI) in rats and probe into the protective role of tacrolimus (FK506) on neural regeneration. Methods Compressive SCI at T8 was induced in the adult SD rats,which were randomly assigned to the control group,FK506 group,NSCs group and NSCs + FK506 group.The differences of neural regeneration in each group were compared at days 7,14,28 and 56 after injury by motor evoked potentials ( MEP),HE staining,immunohistochemical staining,ultrastructure observation and image analysis of the myelinated fiber. Results The MEP latency in the NSCs + FK506 group was significantly shorter than that in other groups at day 28 ( P ＜ 0.05 ).HE staining revealed that only local necrosis presented in the NSCs + FK506 group at day 56.More BrdU and NF-200 positive cells were detected with immunohistochemical staining in the other three groups as compared with the control group.Moreover,the positive cells in the NSCs + FK506 group also outnumbered the FK506 group and NSCs group.Electron microscope scan showed edema under the membrane of large myelin sheath in the control group,and classic new myelin sheath and neuraxis in the NSCs + FK506 group at day 56.The regeneration of the nerve fiber in the NSCs + FK506 group was better than that of other three groups (P ＜0.01 ). Conclusion After NSCs transplantation for SCI rats,the early combination use of FK506 can improve the pathology and ultrastructure of the regenerative nerve fiber and is conducive to nerve regeneration.