中华神经外科杂志
中華神經外科雜誌
중화신경외과잡지
Chinese Journal of Neurosurgery
2009年
8期
750-752
,共3页
吴银艳%黄强%董军%张全斌%季晓燕%朱玉德%刁艺%王爱东%兰青
吳銀豔%黃彊%董軍%張全斌%季曉燕%硃玉德%刁藝%王愛東%蘭青
오은염%황강%동군%장전빈%계효연%주옥덕%조예%왕애동%란청
脑肿瘤干细胞%生物学特征%复发
腦腫瘤榦細胞%生物學特徵%複髮
뇌종류간세포%생물학특정%복발
Brain tumor stem cells%Biological characteristics%Recurrence
目的 比较原发和复发脑肿瘤干细胞的牛物学特征.方法 源于同一患者的原发和复发脑肿瘤干细胞在干细胞培养液中长期传代培养、冻存和复苏,观察其生物学特性.结果 在于细胞培养液中培养出了悬浮的干细胞球,加血清培养时能贴壁分化为多形性瘤细胞.两者都具有分化抑制特性,染色体为异倍体.肿瘤球裸小鼠原位移植,原发为局部侵袭性生长,复发为广泛浸润、播散性生长.结论 对同一患者原发和复发脑肿瘤干细胞生物学特征比较,可为进一步研究肿瘤发生、发展、侵袭和播散等拓展思路.
目的 比較原髮和複髮腦腫瘤榦細胞的牛物學特徵.方法 源于同一患者的原髮和複髮腦腫瘤榦細胞在榦細胞培養液中長期傳代培養、凍存和複囌,觀察其生物學特性.結果 在于細胞培養液中培養齣瞭懸浮的榦細胞毬,加血清培養時能貼壁分化為多形性瘤細胞.兩者都具有分化抑製特性,染色體為異倍體.腫瘤毬裸小鼠原位移植,原髮為跼部侵襲性生長,複髮為廣汎浸潤、播散性生長.結論 對同一患者原髮和複髮腦腫瘤榦細胞生物學特徵比較,可為進一步研究腫瘤髮生、髮展、侵襲和播散等拓展思路.
목적 비교원발화복발뇌종류간세포적우물학특정.방법 원우동일환자적원발화복발뇌종류간세포재간세포배양액중장기전대배양、동존화복소,관찰기생물학특성.결과 재우세포배양액중배양출료현부적간세포구,가혈청배양시능첩벽분화위다형성류세포.량자도구유분화억제특성,염색체위이배체.종류구라소서원위이식,원발위국부침습성생장,복발위엄범침윤、파산성생장.결론 대동일환자원발화복발뇌종류간세포생물학특정비교,가위진일보연구종류발생、발전、침습화파산등탁전사로.
Objective To investigate the biological characteristics of primary and recurrent brain tumor stem cells(BTSCs).Methods Single cell suspensions were made from the primary and recurrent brain tumor tissues,which were obtained from the Surgical specimens of the same patient.then were cultured in medium favored of neural stem cell growth.and were further passaged.frozen.resuscitated and recultured,respectively in vitro.The biological characteristics of the primary and recurrent BTSCs were further investigated.Results In serum-free medium favored for stem cell growth,both of the primary and recurrent BTSCs could form suspending soberes.In medium with serum,BTSCs attached to the bottom of culture dishes and difierentiated into polvmorphic tumor cells.Differentiation jnhibition was observed in both of BTSCs.which were heteroploid.too.Nude mice intracranial xenografi tumors of primary BTSCs exhibited invasive growth around the inoculation region:while tumors of recurrent BTSCs formed multipie disseminated foci and infiltrated the meninges widely.Conclusion Long-term maintaining of the biologically stable primary and recurrent BTSCs will lay the foundation to further investigate the mechanisms of glioma tumorigenesis,growth,invasion and dissemination.
