CAJ | 학술논문

GAP-43,netrin-1,collapsin-1和neuropilin-1被认为在成网络分布的神经联系中发挥重要的作用,在年幼的啮齿类动物中,小脑包含5种不同的集中分布层:白质、内颗粒细胞层(IGL)、浦肯野氏细胞层(PCL)、分子层(ML)和外颗粒细胞层(EGL),与浦肯野氏神经元在出生前产牛这一点不同的是,EGL中的细胞在出生后产生,它们接受从前脑olivary核团发出的攀援纤维的主要神经投射,以及从内颗粒细胞发出的平行纤维的神经投射,这些神经投射主要在出生后的前3个星期内建立,同时还有浦肯野氏细胞的发育和成熟,而GAP-43,netrin-1,collapsin-1和neuropilin-1在出生后小脑发育的潜在作用仍然不清楚,为了更加清楚地探讨上述问题,检验了GAP-43,netrin-1,collapsin-1和neuropilin-1的mRNA与蛋白质在出生后5,10,20天和成年小鼠小脑中的表达情况,研究结果显示,这4种分子在小鼠出生后的小脑中有不同的时间和空间表达形式,这些结果与出生后发育和成年期间的轴突发生、延伸以及突触形成都有关联.通过免疫组织化学双标染色,发现小鼠出生后10天的小脑中,GAP-43阳性的浦肯野氏细胞也显示netrin-l或collapsin-1阳性,并且collapsin-1阳性的细胞也对netrin-1阳性,上述研究结果证明这4种分子可能参与了小脑的出生后发育.
GAP-43,netrin-1,collapsin-1화neuropilin-1피인위재성망락분포적신경련계중발휘중요적작용,재년유적교치류동물중,소뇌포함5충불동적집중분포층:백질、내과립세포층(IGL)、포긍야씨세포층(PCL)、분자층(ML)화외과립세포층(EGL),여포긍야씨신경원재출생전산우저일점불동적시,EGL중적세포재출생후산생,타문접수종전뇌olivary핵단발출적반원섬유적주요신경투사,이급종내과립세포발출적평행섬유적신경투사,저사신경투사주요재출생후적전3개성기내건립,동시환유포긍야씨세포적발육화성숙,이GAP-43,netrin-1,collapsin-1화neuropilin-1재출생후소뇌발육적잠재작용잉연불청초,위료경가청초지탐토상술문제,검험료GAP-43,netrin-1,collapsin-1화neuropilin-1적mRNA여단백질재출생후5,10,20천화성년소서소뇌중적표체정황,연구결과현시,저4충분자재소서출생후적소뇌중유불동적시간화공간표체형식,저사결과여출생후발육화성년기간적축돌발생、연신이급돌촉형성도유관련.통과면역조직화학쌍표염색,발현소서출생후10천적소뇌중,GAP-43양성적포긍야씨세포야현시netrin-l혹collapsin-1양성,병차collapsin-1양성적세포야대netrin-1양성,상술연구결과증명저4충분자가능삼여료소뇌적출생후발육.
GAP-43,netrin-1,collapsin-1,and neuropilin-1 have been regarded to play crucial roles in the formation of patterned neural connections.The cerebellum consists of five distinct concentric layers:white matter,internal granule layer (IGL),Purkinje cell layer (PCL),molecular layer (ML),and external granule layer (EGL) in young rodents.Cells in EGL are generated after birth.In contrast Purkinje neurons are born before birth,which receive main innervations of climbing fibers fi'om the inferior olivary nucleus and parallel fibers from the internal granule cells.These innervations are mostly established in the first three postnatal weeks,accompanying the sprouting and maturation of Purkinje cells.The potential roles of GAP-43,netrin-1,collapsin-1 and neuropilin-1 in the postnatal development of cerebellum remain unclear.To get insights into the above issue,the expression of GAP-43,netrin-1,collapsin-1,and neuropilin-1 mRNAs and proteins were examined in the cerebellum of mice at postnatal days (P) 5,P10,P20 and adulthood.The results showed that these four molecules were expressed in different temporal and spatial patterns in the postnatal cerebellum of mice,which was in match with axonal synaptogenesis,elongation and synapse formation during postnatal development and adulthood.By using double immunohistocbemistry,it was found that the Purkinje cells stained for GAP-43 were also positive for either netrin-1 or collapsin-1 at P10,and cells stained for collapsin-1 were also positive for netrin-1 or neuropilin-1.It was suggested that the four molecules are involved in the postnatal development of cerebellum.