青海医学院学报
青海醫學院學報
청해의학원학보
JOURNAL OF QINGHAI MEDICAL COLLEGE
2009年
2期
130-132
,共3页
张华%李福安%李向阳%李建民%魏全嘉
張華%李福安%李嚮暘%李建民%魏全嘉
장화%리복안%리향양%리건민%위전가
青海%秦艽%花%龙胆苦苷%HPLC
青海%秦艽%花%龍膽苦苷%HPLC
청해%진구%화%룡담고감%HPLC
Qinghai%Gentiana%Flower%Gentiopicroside%HPLC
目的 测定青海道地药材秦艽花中龙胆苦苷的含量.方法 采用反相高效液相色谱法测定不同产地秦艽花中龙胆苦苷的含量.色谱柱为Lichrospher C18(4.6×250 mm,5 μm),流动相为甲醇-水(1∶2 v/v),检测波长为254 nm.结果 在0.54～5.40 μg范围内龙胆苦苷峰面积与浓度线性关系良好(r=0.9994),平均回收率为95.04%(RSD=3.67%),日内、日间精密度分别为1.20%和1.93%.结论 本测定方法灵敏、准确、简便,适用于秦艽花中龙胆苦苷含量测定.
目的 測定青海道地藥材秦艽花中龍膽苦苷的含量.方法 採用反相高效液相色譜法測定不同產地秦艽花中龍膽苦苷的含量.色譜柱為Lichrospher C18(4.6×250 mm,5 μm),流動相為甲醇-水(1∶2 v/v),檢測波長為254 nm.結果 在0.54～5.40 μg範圍內龍膽苦苷峰麵積與濃度線性關繫良好(r=0.9994),平均迴收率為95.04%(RSD=3.67%),日內、日間精密度分彆為1.20%和1.93%.結論 本測定方法靈敏、準確、簡便,適用于秦艽花中龍膽苦苷含量測定.
목적 측정청해도지약재진구화중룡담고감적함량.방법 채용반상고효액상색보법측정불동산지진구화중룡담고감적함량.색보주위Lichrospher C18(4.6×250 mm,5 μm),류동상위갑순-수(1∶2 v/v),검측파장위254 nm.결과 재0.54～5.40 μg범위내룡담고감봉면적여농도선성관계량호(r=0.9994),평균회수솔위95.04%(RSD=3.67%),일내、일간정밀도분별위1.20%화1.93%.결론 본측정방법령민、준학、간편,괄용우진구화중룡담고감함량측정.
Objective To determine the determination of gentiopicroside of gentiana's flower in Qinghai.Methods The concentration of gentiopicroside was determined by RP-HPLC method,the analysis involved a 250 mm × 4.6mm column packed with C18(5 μm),the mobile phase consisted of methyl alcohol-water (1:2).Results A good linearity was obtained in the range of 0.54～5.40 μg (r=0.9994),the average recovery was 95.04%,the relative stand deviations of within-day and between-day were 1.20% and 1.93%.Conclusion The result has demonstrated that it is a sensitive,accurate and simple method for determination of gentiopicroside's flower in gentiana.
