CAJ | 학술논문

[目的]提高大肠杆菌DH5α转化效率.[方法]在其他条件一致的情况下,将5 μl的质粒或连接产物加入含有100 μl 的感受态0.2 ml PCR管和1.5 ml的离心管中,0.2 ml PCR管设置热休克时间梯度和1.5 ml的离心管热休克时间90 s,对大肠杆菌DH5α转化效率进行了比较.[结果]0.2 ml PCR管转化效率高于1.5 ml的离心管,且0.2 ml PCR管热休克30 s时最高.[结论]采用此方法提高了大肠杆菌DH5α的转化效率.
[목적]제고대장간균DH5α전화효솔.[방법]재기타조건일치적정황하,장5 μl적질립혹련접산물가입함유100 μl 적감수태0.2 ml PCR관화1.5 ml적리심관중,0.2 ml PCR관설치열휴극시간제도화1.5 ml적리심관열휴극시간90 s,대대장간균DH5α전화효솔진행료비교.[결과]0.2 ml PCR관전화효솔고우1.5 ml적리심관,차0.2 ml PCR관열휴극30 s시최고.[결론]채용차방법제고료대장간균DH5α적전화효솔.
[Objective]The research aimed to improve the transformation efficiency of E.coli DH5α.[Method]Under the same other conditions, 5 μl plasmid or legation product was added into 0.2 ml PCR tube and 1.5 ml centrifuge tube with 100 μl competent in them. In different thermal shock time, transformation efficiency of E.coli DH5α was compared.[Result]Transformation efficiency with 0.2 ml PRC reformer tube is better than that with 1.5 ml centrifuge tube and the 0.2 ml PCR reformer tube with 30 seconds' thermal shock time gives the best performance of transformation efficiency.[Conclusion]Transformation efficiency of E.coli DH5α is effectively improved by this method.