CAJ | 학술논문

为了解海洋底柄甲藻--利玛原甲藻(Prorocentrum lima)的产毒特征,阐明腹泻性贝毒(Diarrhetic shellfish poisoning,DSP)复杂的生物合成机制,采用酶联免疫试剂盒,对利玛原甲藻不同生长期、不同营养盐条件下毒素生成情况进行了研究.结果显示,不同生长期单个利玛原甲藻细胞中大田软海绵酸(Okadaic acid,OA)的含量明显不同,平台期时OA含量最高,与对数中期存在显著差异(p<0.05).低N、P条件下藻的生长状况较差,所能到达的藻密度明显低于对照组(f/2培养基);藻细胞中OA的总含量也低于对照组,但单个藻细胞中OA的含量明显高于对照组(p<0.05).以上结果提示,利玛原甲藻DSP毒素的产生可能与藻所处的环境和藻的生理状态等有关.
위료해해양저병갑조--리마원갑조(Prorocentrum lima)적산독특정,천명복사성패독(Diarrhetic shellfish poisoning,DSP)복잡적생물합성궤제,채용매련면역시제합,대리마원갑조불동생장기、불동영양염조건하독소생성정황진행료연구.결과현시,불동생장기단개리마원갑조세포중대전연해면산(Okadaic acid,OA)적함량명현불동,평태기시OA함량최고,여대수중기존재현저차이(p<0.05).저N、P조건하조적생장상황교차,소능도체적조밀도명현저우대조조(f/2배양기);조세포중OA적총함량야저우대조조,단단개조세포중OA적함량명현고우대조조(p<0.05).이상결과제시,리마원갑조DSP독소적산생가능여조소처적배경화조적생리상태등유관.
In order to explore the feature of Diarrhetic shellfish poisoning(DSP) production and elucidate the complicate mechanism of DSP biosynthesis,production of DSP in different growth phases in Prorocentrum lima and under different nutrients conditions was investigated by ELISA.Okadaic acid (OA) concentrations per cell of P.lima were different in various growth phases.The peak of OA was in the stationary phase,which was different significantly to that in the mid-log phase(p<0.05).P.lima under low concentrations of N,P grew worse than control,while the maximum densities were less than that of control.However.OA concentrations per cell under low concentrations of N,P were higher than that of control.These results suggested that the DSP synthesis in P.lima was related to the environment stress and algal physiological state.